四种重楼属植物光合作用特征

Species of Paris (Trilliaceae) have often been used as medicinal plants. Because of excessive exploitation,in this regard the wild resource of Paris is almost exhausted. Some species of Paris were transplanted for use in photosynthesis research and for conservation purposes. In the present study, light and CO2 photosynthetic response curves were investigated in four Paris taxa: P.polyphylla var. yunnanensis and var. alba, P.mairei, and P.marmorata. Our results showed that P.marmorata had the highest maximum photosynthetic rate (Pmax; 8.6μmol·m-2·s-1), light saturation point (LSP; 827μmol·m-2·s-1), maximum electron transport rate (Jmax; 39.9mol·m-2·s-1), a relatively high maximum carboxylation rate (Vcmax; 28.9μmol·m-2·s-1) and carbon dioxide saturation point (Cisat; 726μmol·mol-1), but a lower light compensation point (LCP; 6.23μmol·m-2·s-1) and the lowest carbon dioxide compensation point (Г*; 20.7μmol·mol-1). This suggests that P.marmorata is well adapted to light and CO2; however it has a low ability to acclimate to environmental stress as indicated by low water use efficiency (WUE) in high light conditions. P.polyphylla var. yunnanensis had the highest light compensation point (LCP; 10.1μmol·m-2·s-1), carbon dioxide compensation point (Г*; 35.3μmol·mol-1), carbon dioxide saturation point (Cisat; 727μmol·mol-1), relatively high maximum photosynthetic rate (Pmax; 7.5μmol·m-2·s-1) and light saturation point (LSP; 728μmol·m-2·s-1), maximum light saturated electron transfer rate (Jmax; 37.7μmol·m-2·s-1), suggesting that it is suitable for conditions of higher light and CO2 concentration. This taxon can adapt to adverse conditions, as suggested by high WUE under increased CO2 concentration. In contrast, P.polyphylla var. alba exhibited a relatively lower apparent quantum yield (AQY; 0.037μmol·mol-1) and poorer growth performance than the other taxa. We suggest from our results that different light and water conditions are suitable for the growth of the different taxa. Photosynthesis assimilation efficiency and production can be increased by raising humidity for P.polyphylla var. yunnanensis and P.marmorata. To protect plants of P.polyphylla var. alba from strong sunshine, they should be shaded from March to mid June.     

金铁锁驯化栽培技术

在对金铁锁人工驯化栽培技术多年的试验研究基础上,总结了金铁锁驯化栽培管理技术。     

云南重楼的组织培养与植株再生

1植物名称云南重楼[Paris polyphylla Smith vaY.yunnanensis(Franch.)Hand.-Mazz.]. 2材料类别芽. 3培养条件愈伤组织诱导培养基:(1)MS 6-BA 2 mg·L-1(单位下同) NAA 0.1;愈伤组织增殖和分化培养基:(2)MS 6-BA 2 NAA 0.5 KT 0.5;生根培养基:(3)I/2MS NAA 0.5 IAA 0.5.上述培养基中均附加3%蔗糖和0.7%琼脂,pH 5.8,在121℃下高压灭菌20 min.培养温度控制在(20±2)℃,光照时间8 h·d-1,光照强度20～30μmol·m-2·s-1.     

滇西北野生长柱鹿药的资源分布与保护利用

报道了滇西北野生长柱鹿药的资源分布状况、开发利用现状及存在的问题,提出了开发与保护可持续发展的措施.     

临床用药风险管理常见问题及应对措施

目的：对我院不安全用药行为进行监测与调查,分析临床用药问题中存在的风险因素,探讨医疗机构加强药品风险管理的措施,以减少临床用药的安全隐患。方法：采用调查问卷的方式,对我院部分科室就诊患者的用药情况进行问卷调查;应用“医院用药安全监测与评价”软件对我院医护人员在临床用药管理方面存在的风险因素进行评估,整理并分析调查结果。结果：在本次临床用药风险分析中,各因素所占比例为：医生：30.50%,药师：9.00%,护士：32.00%,患者：28.50%。了解临床用药各环节中存在的临床用药风险的医护人员占83.3%,但只有50.6% 的人员采取基本的防范措施。用药风险警示调查显示：PASS 警示有效率为57.5%;关联因素评价中人员因素的发生率最高55.16%;外界因素次之31.29%;药物因素发生率为26.95%。结论：医疗机构应当加强临床用药管理,识别、分析用药风险因素,强化合理用药意识及知识,努力构建临床用药风险控制系统,为医药护理人员及患者营造良好的医疗安全氛围,从而降低用药风险的发生,保障患者的用药安全。