Amplification, analysis and chromosome mapping of novel homeobox-containing and homeobox-flanking sequences in rice

Homeobox genes, widely distributed among animal and plant kingdoms, play an important role in developmental process. Several homeobox conserved fragments were amplified by PCR and the flanking regions were also obtained by an LM-PCR procedure. Sequencing and Southern analysis showed that they belong to a homeobox gene family of rice. Six homeobox-containing fragments were mapped on the molecular linkage map of rice. They were located on chromosomes 3, 4 and 7 respectively. It is noteworthy that there are 4 homeobox fragments located on rice chromosome 3 and the result is also consistent with the comparative genomics between rice and maize.     

野生火棘果红色素提取工艺优化

目的:通过正交实验,确定火棘果红色素的最佳提取工艺。方法:以贵州野生火棘果为原料,首先,以柠檬酸的乙醇溶液为提取剂,在400～800nm范围内对提取液的吸收光谱进行扫描,确定其最大吸收波长;然后,以最大吸收波长处的吸光度为衡量指标,确定最佳提取剂;再次,探讨了提取次数、提取温度、提取时间、料液比等单因素对火棘果红色素提取效果的影响;最后,在单因素实验结果的基础上,通过L9(34)正交实验,确定红色素的最佳提取工艺。结果:1.在400～800nm范围内,火棘果红色素的最大吸收波长为530nm;2.最佳提取剂为9%柠檬酸乙醇溶液;3.火棘果红色素的最佳提取工艺为,料液比1∶10,提取时间60min,提取次数2次,提取温度40℃。     

纳米药物在癌症诊疗中的应用进展

癌症的诊断和治疗在近年取得了重大进步,但癌症目前仍是影响人类生存和健康的主因之一。提高癌症的早期诊断率和治疗效果具有重要意义。近来纳米技术在医学领域发展迅速,突破了传统癌症诊疗手段的固有局限性,并在临床上取得了一定成功。已有多个代表性药物获得新药批准或正处于临床试验阶段。然而,纳米药物仍面临肿瘤生物学的复杂性和异质性、药物安全性、纳米-生物界面的相互作用等挑战。该文阐述了近期纳米药物在肿瘤诊断以及化疗、放疗、光疗、化学动力治疗、免疫治疗和联合疗法等方面的应用进展,讨论了其在临床转化、生产和商业化所面临的问题,并对未来的发展机会和方向进行了展望。     

水稻同源盒序列的扩增、分析及染色体定位

广泛存在的同源盒基因家族与生物的发育控制过程有关 .用特异PCR扩增获得同源盒保守序列 ,并通过LM PCR方法获得保守序列的侧翼序列 ,进而对克隆的扩增片段进行序列分析和Southern分析 ,并在水稻的分子连锁遗传图上定位了 6个同源盒序列 .它们分别被定位在水稻第 3 ,4和第 7染色体上 ,在第 3染色体上共定位了 4个同源盒序列 .值得注意的是水稻同源盒序列定位的结果与玉米已知的同源盒基因的定位结果相当符合 .