血清样本双向电泳操作条件的优化

为建立分辨率高、重复性好的血清样品双向电泳技术,本文从血清样品的水化、等电聚焦、胶条的平衡、胶条的染色等几个方面对双向电泳操作条件进行了分析。结果表明采用以下的操作过程可以获得分辨率高、重复性好的双向电泳结果：样品水化2小时（25℃);胶条泡涨12-16小时(25℃);17cm胶条的等点聚焦程序采用 250V线性1小时/1000V线性1小时/4000V线性2小时/8000V线性3小时/8000V线性10小时/500V快速0.5小时/,11cm的胶条的等点聚焦程序采用250V慢速4小时/1000V快速2小时/4000V快速1小时/8000V快速2.5小时/8000V快速7小时/500V快速30分钟;两次平衡各15-20分钟;银染条件为固定两小时或过夜,敏化50-60分钟,定影30-40分钟,显影10分钟左右,终止10分钟）。这一研究对利用双向电泳分析血清蛋白具有很好的参考价值。     

中国畲族群体D17S30位点遗传多态性研究

采用聚合酶链反应 (PCR)技术对中国畲族群体270名无关个体D17S30位点的VNTR进行了研究,共检出12个等位基因,片段大小为168bp-1008bp,基因频率为0.0056-0.3259,杂合度为79.10%,父权排除率为0.4358。家系分析表明,扩增片段按孟德尔方式遗传。统计学分析证明,本资料符合Hardy-W einberg平衡定律(x2=79.97,v=66,P=0.1158>0.05)。 Abstract:The variable number of tandem repeat(VNTR)of D17S30 locus in 270 unrelated individuals of She ethnic group was studied by polymerase chain reaction(PCR).The results showed that 12 alleles were detected and their sizes ranged from 168bp to 1 008bp.The allele frequencies were 0.0056~0.3259,the heterozygosity of this locus was 79.10% and the paternity exclusion probability was 0.4358.The number of D17S30 VNTR allele and allele frequency of She were different from those of Han ethnic group in Mendelian Law.Statistical analysis demonstrated that their geneotypes were consistent with Hardy-Weinberg equilibrium(x2=79.97,v=66,P=0.1158>0.05).