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ABSTRACT: Three diets supplemented with taurine, β-alanine andGABA and a control diet were fed to juvenile and fingerling Japaneseflounder to investigate the effects of the diets on growth and metabolicchanges of free amino acids in whole body and tissues. In experimentI, three diets supplemented with 1% each of taurine, β-alanineand GABA and a control diet were fed to juvenile Japanese flounderwith an initial mean body weight of 0.4 g for 4 weeks at20°C. In experiment II, the taurine-supplemented diet anda control diet were fed to fingerling Japanese flounder with an initialmean body weight of 15 g for 4 weeks at 20°C.Only supplementation of taurine in the diet of juvenile flounderimproved their growth performance in experiment I, but fingerlinggrowth performance of experiment II was not significantly relatedto taurine supplementation in the experimental diet. These resultssuggest that there is a greater requirement for taurine for thegrowth of juvenile Japanese flounder than fingerling Japanese flounder. 相似文献
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CHIAKI IMADA YUKO IKEMOTO TAKESHI KOBAYASHI NAOKO HAMADA ETSUO WATANABE 《Fisheries Science》2002,68(2):395-402
ABSTRACT: Protoplast fusion between different species of Streptomyces was performed using a liquid regeneration method developed for a rapid and simple preparation of the fusants. Consequently, new clones, which could not be obtained using the conventional agar regeneration method, were obtained. In the crosses between S. griseus and S. durhamensis , and between S. californicus and S. catenulae , eight and two recombinants, respectively, were obtained using the liquid regeneration method. Conversely, in the case of crosses between S. ornatus and S. catenulae , and between S. ornatus and S. vendargensis , seven recombinants each were obtained using only the agar method. The physiological characteristics, such as the assimilation of carbohydrate and antibiotic resistance, of these fusants differed considerably from those of their parental strains. Using the proposed liquid regeneration method, a simpler and quicker procedure for protoplast fusion is described. 相似文献
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Kunio SATOU Jun-ichi SUTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):753-759
We investigated the effect of the Y chromosome on testis weight in
(B6.Cg-Ay × Y-consomic mouse strain) F1 male
mice. We obtained the following results: (1) Mice with the Mus musculus
domesticus-type Y chromosome had significantly heavier testis than those with
the M. m. musculus-type Y chromosome. (2) Variations in
Usp9y and the number of CAG repeats in Sry were
significantly associated with testes weight. The Ay allele was
correlated with a reduced testis weight, and the extent of this reduction was
significantly associated with a CAG repeat number polymorphism in Sry.
These results suggest that Y chromosome genes not only influence testis weight but also
modify the effect of the Ay allele in mediating this
phenomenon. 相似文献
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Jun-ichi SUTO Kunio SATOU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):203-210
The Ay allele at the agouti locus causes obesity and promotes linear growth in mice. However, body weight gain stops between 16 and 17 weeks after birth, and then, body weight decreases gradually in DDD.Cg-Ay male mice. Body weight loss is a consequence of diabetes mellitus, which is genetically controlled mainly by a quantitative trait locus (QTL) on chromosome 4. This study aimed to further characterize diabetes mellitus and body weight loss in DDD.Cg-Ay males. The number of β-cells was markedly reduced, and plasma insulin levels were very low in the DDD.Cg-Ay males. Using a backcross progeny of DDD × (B6 × DDD.Cg-Ay) F1-Ay, we identified one significant QTL for plasma insulin levels on distal chromosome 4, which was coincidental with QTL for hyperglycemia and lower body weight. The DDD allele was
associated with decreased plasma insulin levels. When the DDD.Cg-Ay males were housed under three different housing conditions [group housing (4 or 5 DDD.Cg-Ay and DDD males), individual housing (single DDD.Cg-Ay male) and single male housing with females (single DDD.Cg-Ay male with DDD.Cg-Ay or DDD females)], diabetes mellitus and body weight loss were most severely expressed in individually housed mice. Thus, the severity of diabetes and body weight loss in the DDD.Cg-Ay males was strongly influenced by the housing conditions. These results demonstrate that both genetic and nongenetic environmental factors are involved in the development of diabetes mellitus and body weight loss in the DDD.Cg-Ay males. 相似文献
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YUKO SHIMAMOTO NAOFUMI NOMURA TOKUSHIRO TAKASO HIROAKI SETOGUCHI 《Weed Biology and Management》2011,11(3):118-126
The invasion of natural ecosystems by exotic species is a major threat to biodiversity on a local, regional, and global scale. Endemic island flora are particularly vulnerable to invasive species. Bidens pilosa var. radiata is a common invasive weed of roadsides, which are subject to clipping, on Japanese subtropical islands. To assess compensation by the weed to damage by clipping, a pot experiment was conducted under common garden conditions. The above‐ground parts were clipped with pruning shears at various frequencies, ranging from no clipping (the control) to clipping at 4–16 week intervals for 32 weeks in summer. The clipped individuals regenerated new branches from the axillary buds and flowered for ≤12 weeks after the clipping. The regeneration after the clipping gave rise to secondary reproduction, whereas minimal regeneration occurred in the unclipped individuals after the maturation of the fruit. Consequently, the total flower head mass, represented by the dry weight of the inflorescences throughout the experiment, was higher on the individuals that were clipped at 12 and 16 week intervals than on the unclipped individuals, suggesting overcompensation in fecundity. At the higher frequencies of above‐ground clipping, the flower head mass was suppressed at 8 week clipping intervals, while both the flower head mass and the productive biomass were suppressed at 4 week clipping intervals. This indicates that clipping at intervals of ≤8 weeks is required to prevent the reproduction of this weed. 相似文献
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Jun-ichi SUTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):353-358
We performed quantitative trait locus (QTL) mapping analysis for litter size (total
number of pups born and/or number of pups born alive) in 255 backcross mice derived from
C57BL/6J and RR/Sgn inbred mice. We identified one significant QTL on chromosome 7 and 4
suggestive QTLs on chromosomes 3, 5, 10 and 13. In addition, two suggestive QTLs were
identified on chromosomes 1 and 4 for the number of stillbirth. These results suggested
that both litter size and number of stillbirth were heritable traits, although they were
controlled by distinct genes. The RR allele was associated with reduced litter size and
increased stillbirth at all QTLs. Therefore, RR mothers were observed to have reduced
prolificacy in this particular genetic cross. 相似文献
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Jun-ichi SUTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):809-813
Newborn offspring of the inbred mouse RR/Sgn strain have a low survival rate prior to
weaning. We hypothesized that this is a consequence of an inferior nurturing ability of
RR/Sgn mothers and that RR/Sgn mothers have a tendency to lose their pups. We performed
quantitative trait locus (QTL) mapping for inferior nurturing ability and tendency to lose
pups in RR/Sgn mothers. The number of pups was adjusted to 6 per dam on the day of
delivery, and the number of surviving pups and their total weight (litter weight) were
scored at 12 days after birth. Nurturing ability was evaluated by litter weight, and
tendency to lose pups was evaluated by scoring whether or not the mothers lost their pups.
For litter weight, we identified one significant QTL on chromosome 4 and three suggestive
QTLs on chromosomes 7, 9 and 17. The RR/Sgn allele was associated with lower litter weight
at all loci. For the tendency to lose pups, we identified three suggestive QTLs on
chromosomes 4, 9 and 16. The RR/Sgn allele was associated with an increased tendency to
lose pups at all loci. These results supported our hypothesis that the low survival rate
phenotype was attributable, at least in part, to a phenotype whereby mothers display
inferior nurturing ability and a tendency to lose pups. Thus, it suggests that these two
traits share genetic basis. 相似文献
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Akemi SUTO Yukinori SUTO Nozomi ONOHARA Yu TOMIZAWA Yukiko YAMAMOTO-SUGAWARA Taro OKAYAMA Kenichi MASUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):251-254
Canine atopic-like dermatitis (ALD) is suspected to be associated with food allergies, particularly those mediated by lymphocytes. In this study, 54 cases were included as ALD dogs, based on the negative IgE test results. In the dogs, the percentage of activated cells in helper-T lymphocytes was measured by flow cytometry using cultured peripheral lymphocytes under food allergen stimulation. We observed that 49 of the 54 ALD dogs (90.7%) had positive lymphocyte reactions against one or more food allergens. The most common food allergen was soybean, showing positive results in 21 dogs (42.9%), while the allergen to cause the lowest number of reactions was catfish (only 5 dogs, 10.2%). These results may be useful in considering elimination diets for ALD dogs. 相似文献