玉米抗粗缩病遗传及分子标记研究

Maize rough dwarf disease caused by Rice black-streaked dwarf virus (RBSDV) is transmitted by planthopper in China. Identification and development of resistant hybrids are complicated because of the inconsistencies in viral disease pressure every year. Marker-assisted selection can provide means for main-taining virus resistance alleles even in the absence of disease. In this paper a F₂ segregation population was constructed to identity the molecular markers linked to the resistance gene using a cross between a resistant and a susceptible parents (Qi319×Ye107). Fifteen-day-old seedlings of F₂ population were exposed to small brown planthoppers carrying RBSDV for 3 days in specific inoculation chamber. The inoculated plants were transplanted to screenhouse after removing the insects completely. In plant maturity stage the disease resistance of all the individuals were visually assessed. The results showed that 17, 8, 11, 51 and 122 plants were scaled from 0-4 respectively, in which 0 means no symptoms and 4 represents highly susceptible. Chi-square test demonstrated that the segregation ratio of phenotype was 1∶15 (resistant: susceptible) or 1∶6∶9 (resistant∶moderate∶susceptible) in the F₂ population, indicating RBSDV resistance of maize was controlled by two recessive genes. The F₂ individuals DNA were extracted and 261 SSR (simple sequence repeat) primers derived from maize genome ten chromosomes were selected from maize GDB database to construct genetic linkage map. The linkage map consisted of 71 polymorphic SSR markers, spanning a genetic distance of 996.6 cM with an average interval of 14.0 cM between adjacent markers. The resistant and susceptible gene pools were set up for BSA (bulked segregant analysis) and 6 polymorphism markers were obtained with BSA-SSR method between the two pools. The F₂individuals were further analyzed with 6 polymorphism markers. Chi-square test showed that phi 051, umc1407 and umc1432, mapped on chromosome 7 and 10, exhibited segregation distortion significantly and very significantly in susceptible individuals. These three SSR markers were identified as potential markers linked to the resistant loci.     

关于大兴安岭地区绿色产业发展的思考

分析了大兴安岭绿色特色产业发展现状,指出了存在的问题,并提出发展绿色产业的6点构想。     

红花黄色素超声辅助提取工艺优化及其稳定性

[目的]探究超声辅助热水提取红花黄色素的最佳工艺,为实现红花黄色素的绿色高效提取提供参考.[方法]以在我国广泛生长的菊科植物红花为试验材料,以去离子水为提取溶剂,以超声处理为辅助提取手段,以红花黄色素的提取率为试验指标,探究不同提取液pH、超声功率、超声时间、水浴温度、水浴时间对红花黄色素提取率的影响.基于单因素试验结...     

高温对茶树叶片光合及抗逆特性的影响和恢复*3

以4个茶树品种福鼎大白茶、鸠坑、龙井43和乌牛早7a生植株为研究对象,采用人工气候箱模拟高温（35℃和40℃）处理6、12、18、24、48h,取出后置于人工气候箱中（25℃）恢复3、6、9d,以未经高温处理置于人工气候箱中（温度25℃）的各品种茶树为对照（CK）,测定茶树叶片的最大净光合速率、荧光参数、抗氧化酶活性以及细胞伤害率。结果表明：高温胁迫显著抑制了茶树的最大净光合速率（Pnmax）和最大光化学效率（Fv/Fm）,处理时间越长、温度越高,Pnmax和Fv/Fm下降越快,除35℃处理的福鼎大白茶外,其它3种茶树经过高温处理后,在恢复期间其Fv/Fm无法恢复至正常水平;茶树叶片的SOD酶活性在高温处理的前12h迅速上升,随着处理时间的延长,其活性降低;叶片MDA含量的平均值在高温处理第48小时达到最大,恢复期间缓慢下降;随着处理温度的升高和处理时间的延长,各茶树叶片的细胞伤害率均呈增加趋势。4种茶树耐热性的强弱由高到低依次为福鼎大白茶＞乌牛早＞鸠坑＞龙井43。     

玉米新品种鲁单718的选育及其栽培技术要点

鲁单718是以齐3925为母本、黄572为父本（或其反交）杂交选育而成。该品种具有高产、优质、多抗、适应性广等特点,适宜四川平丘区和黄淮海地区春夏播种植。     

基于模糊控制的阀门定位器控制系统研究

为了提高阀门定位器的调节精度,文章结合阀门定位器的结构特点和工作环境,提出基于智能控制策略的阀门定位器控制系统方案。通过Matlab的Simulink模块,对应用不同控制算法的PID控制器的定位器系统进行仿真,得出各自的单位阶跃响应曲线,对比后可知,模糊PID算法能很好地改善阀门定位器控制系统性能。     

2008年天津口岸进境原木情况分析

2008年3～12月天津口岸进境原木130批次,共23156.2544 m3,其中71%的进境原木流向华北地区.进境原木主要来自德国、新西兰、澳大利亚、美国、俄罗斯和加拿大等29个国家和地区.进境树种有辐射松、山毛榉、落叶松、红松等45种.针对原木的检疫处理方式,29个国家和地区中有12个国家和地区采用溴甲烷熏蒸,10个国家采用磷化氢熏蒸,部分国家采用硫酰氟熏蒸和化学农药喷洒处理.     

适于机械化粒收玉米新品种育种策略探讨

随着山东省玉米生产的发展,玉米机械化收获水平越来越高。从人工收获到机械收穗,再到机械粒收,是玉米收获的重要变革。收获方式的转变对玉米品种选育提出了新的要求,结合山东省玉米机械化收获发展状况,探讨了适宜机械化粒收的玉米自交系和杂交种选育目标,以期为育种工作提供参考。     

发挥政府职能推进茶产业发展

传统茶文化发展至今已经作为一种新型创新产业越出于当下经济社会。特别是在中国——茶的故乡。茶产业的发展都显得尤为重要。然而作为一项古往今来众人研究的产业,发展空间是否存在局限,是否还有机会进行拓展,这都与政府职能的实施有着重要联系。本文就对政府职能发挥对茶产业发展的影响进行论述。     

鹿角托盘总蛋白的提取工艺研究

[目的]优化鹿角托盘总蛋白提取工艺。[方法]以总蛋白得率为评价指标,采用单因素试验和正交试验设计优选最佳提取工艺。[结果]最佳提取工艺为：用含0.3mol/LNaCl,20mmol/LNa2HPO4缓冲液（pH值为9）提取,料液比为1：15,提取时间为24h,提取3次。在此工艺条件下,总蛋白得率达10%以上。[结论]试验优选出的工艺稳定、合理、可行。