模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.     

57例鼻腔NK/T细胞淋巴瘤临床分析

目的:探讨鼻腔NK/T细胞淋巴瘤的临床表现、放疗和化疗的近期疗效以及不同的治疗方法对生存率的影响。方法:收集我院1994年2月～2002年12月收治的57例经病理形态学诊断为鼻腔NK/T细胞淋巴瘤患者的临床资料,主要表现为鼻塞、涕血、恶臭。采用单纯放疗或放疗后化疗35例,化疗后放疗22例,观察及治疗效果。结果:近期疗效:35例经单纯放疗或放疗后化疗,放疗后完全缓解(CR)率为74%,其余22例为化疗后放疗,化疗后CR率仅23%,单纯放疗或放疗后化疗的CR率明显高于化疗后放疗的CR率(P<0.01),全组患者中先放疗组、化疗后放疗组患者的5年生存率分别为60%和55%(P>0.05)。结论:鼻腔NK/T细胞淋巴瘤的临床表现不典型,单纯放疗或放疗后化疗的近期疗效显著优于常规化疗,化疗加入放疗并未改善生存率。     

模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.     

模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.     

模拟IMRT对CNE-2细胞周期及Cyclin D1/Cyclin B1表达的影响

目的 探讨模拟调强放射治疗(IMRT)对人鼻咽低分化鳞癌细胞株(CNE-2)细胞周期及细胞周期素Cyclin D1、Cyclin B1转录水平的影响.方法 CNE-2分为急速照射(ART)组和模拟IMRT组,两组分别给予6MV X线2、4、6和8 Gy 四个剂量点的照射;ART组的照射时间为1～3 min,模拟IMRT组的完成时间为35 min.另设空白对照组,不接受照射,其余条件相同.照射后12 h,流式细胞术分析细胞周期分布,RT-PCR检测细胞周期素Cyclin D1和Cyclin B1的转录水平.结果 在相同剂量点,模拟IMRT组G2期细胞比例均低于ART组,两组G2期细胞比例随照射剂量的增加而逐渐增加.模拟IMRT组与ART组在相同剂量点之间比较,Cyclin D1转录水平的差异均无统计学意义(P均＞0.05);Cyclin B1转录水平的差异均有统计学意义(P均<0.05),且随照射剂量的增加而下降,模拟IMRT组Cyclin B1的表达高于ART组.结论 模拟IMRT照射时间延长对G2期阻滞作用下降,细胞周期素Cyclin B1的表达相对升高.     

不同Stat6表型乳腺癌细胞IL-4/Stat6信号通路调节基因表达的研究

目的:探讨不同乳腺癌细胞株Stat6活化分型的可行性及IL-4的作用机制.方法:流式细胞仪测定不同Stat6表型细胞株ZR-75-1和BT-20的早期凋亡率;应用Affymetrix基因芯片测定IL-4刺激前后的基因表达谱变化.结果:Stat6null表型细胞与Stat6high相比早期凋亡增加(40%vs 12%);在Star6null细胞株有2 193个基因/转录产物表达升高,而Stat6high细胞株中2 600个基因/转录产物表达升高,且Stat6high细胞和Stat6null细胞中与凋亡和转移相关的基因表达谱明显不同;但不论在Stat6high还是Stat6null细胞株,IL-4均上调CCL26、SOCS1、CISH、EGLN3和SIDT1基因,而下调DUSP1、FOS和FOSB基因.结论:在乳腺癌细胞中,IL-4可能像在免疫细胞中一样,通过Stat6或非Stat6途径而发挥功能.     

模拟IMRT模式下相对剂量率变化对CNE-2细胞周期及Cyclin D1、Cyclin B1蛋白表达的影响

目的探讨模拟调强放射治疗（intensity-modulated radiation therapy IMRT）对人鼻咽低分化鳞癌细胞株（CNE-2）细胞周期及Cyclin D1、Cyclin B1蛋白水平的影响。方法选取CNE-2为实验对象,分为空白对照组、急速照射组（acute radiation AR）及模拟IMRT（simulational intensity-modulated radiation SIMR）组,后2组分别给予6MV-X线2、4、6、8 Gy 4个剂量点的照射。急速照射组完成时间1-3分钟;模拟IMRT组：各个剂量点分别等分分割5次,每次间隔8-8.5分钟,总时间35分钟照射完成。应用流式细胞术（FCM）分析细胞周期再分布的差异;应用Western blotting检测细胞周期相关因子Cyclin B1、Cyclin D1的蛋白水平。结果在相同剂量点,模拟IMRT组G2期细胞比例低于急速照射组,G2期细胞比例随照射剂量的增加而逐渐增加。急速照射与模拟IMRT组不同剂量点之间以及在相同剂量点比较,Cyclin D1的蛋白表达差异均无统计学意义（P值均〉0.05）;Cyclin B1的蛋白表达差异均有统计学意义（P值均〈0.05）,且随照射剂量的增加而下降,模拟IMRT组Cyclin B1的表达高于急速照射组。结论模拟IMRT照射时间延长,对G2期阻滞作用下降,细胞周期素Cyclin B1的表达相对升高。     

结直肠癌细胞IL-4／Stat6信号传导活性与调节基因差异性表达相关性研究

目的：IL-4介导的Star6信号传导活性在人细胞之间有差异，称为Stat6活化表型。旨在探讨Stat6活化表型及其产生的分子学基础。方法：结直肠癌细胞株HT-29与Caco-2的Star6活化表型用EMSA半定量法测定。Stat6通路调节因子基因水平的表达用RT-PCR法检测。蛋白水平的表达用Western blotting法检测。结果：EMSA分析表明，HT29为高活化表型（Star6^high）而Caco2为零活化表型（Star6^null）。RT-PCR分析显示，与Star6^high HT-29比较，Star6^null Caco-2癌细胞高表达负调节基因SOCS1和SHP1，而低表达正调节因子PP2A催化亚单位编码基因PP2CA和PP2CB。Westernb lotting分析法证实Caco-2癌细胞高表达SOCS1和SHP1蛋白。结论：Star6通路正、负调节基因的表达失衡可能是产生不同Star6活化表型的分子机制之一。     

模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.     

模拟调强技术照射鼻咽低分化鳞癌细胞的生物效应机制研究

Objective To study the altered radiobiological effect of simulative intensity-modulated radiotherapy (SIMR) in cultured human nasopharyngeal carcinoma (NPC) cells and the related mechanism. Methods Single cell suspension of exponentially growing CNE-2 cells, a poor differentiated NPC cell line, was seeded and cultured for 12 hours, then the cells were irradiated in two different models by 6 MV X-ray beams at 3 Gy/min. In single fraction irradiation (SFR) model, cells were irradiated a single fraction of 0, 2, 4, 6 or 8 Gy within 0 to 3 minutes. In S1MR model, cells were irradiated 0, 2, 4, 6 or 8 Gy in 5 frac-tions with interval of 8.0-8.5 minutes between. Clonogenic assay was performed to determine the radiosen-sitivity. Cellular apoptosis was measured by flow cytometry. RT-PCR was used to detect mRNA expressions of Bax and Bcl-2, Respectively. Results Compared with SFR group, the survival fraction in SIMR group was higher at all the dose levels. The values of α, β, D0 and Dq were higher in SIMR group than in SFR group. At dose levels of 2 Gy, 4 Gy and 6 Gy, The early and late apoptotic cells in SIMR group were lower than in SFR group (21.20%: 15.89%, F=18.51, P=0.020;13.00%: 10.20, F=15.67, P=0.040).The mRNA expression of Bax was upregulated in a dose-dependent manner in the both groups. Compared with SFR group, the mRNA expression of Bax in SIMR group was lower at all the dose levels (Mean value of 76.75% : 62.50%, F =36.57, P =0.000). Bcl-2 mRNA expression at every dose level had no significant difference between the two groups (Mean value of 29.25% : 29.75%, F=0.74, P=0.800). Conclusions Prolonged delivery time in SIMR model can decrease the radiobiological effects.