全文获取类型
收费全文 | 356篇 |
免费 | 16篇 |
学科分类
工业技术 | 372篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 6篇 |
2019年 | 5篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 5篇 |
2013年 | 30篇 |
2012年 | 13篇 |
2011年 | 16篇 |
2010年 | 14篇 |
2009年 | 11篇 |
2008年 | 10篇 |
2007年 | 12篇 |
2006年 | 11篇 |
2005年 | 14篇 |
2004年 | 12篇 |
2003年 | 18篇 |
2002年 | 9篇 |
2001年 | 5篇 |
2000年 | 5篇 |
1999年 | 6篇 |
1998年 | 25篇 |
1997年 | 25篇 |
1996年 | 17篇 |
1995年 | 7篇 |
1994年 | 8篇 |
1993年 | 12篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1977年 | 4篇 |
1976年 | 1篇 |
排序方式: 共有372条查询结果,搜索用时 15 毫秒
1.
Tadashi Hano Michiaki Matsumoto Takahisa Kawazu Takaaki Ohtake 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1995,62(1):60-63
The extraction equilibria of various di- and tripeptides with di-2-ethylhexylphosphoric acid (D2EHPA) were studied at low pH values. The complex extracted to organic phase consisted of one molecule of peptide and two molecules of D2EHPA dimer. The extraction constants of the peptides correlated well with the distribution coefficients of peptides between 1-octanol and water, which is a measure of hydrophobicity. The permeation rates of peptides through an emulsion liquid membrane were examined by using D2EHPA as a carrier, Span 80 as an emulsifier and kerosene as a diluent. The rates varied considerably with peptide type, depending upon the hydrophobicity. 相似文献
2.
Of the four genes (nrtABCD) required for active transport of nitrate in the cyanobacterium Synechococcus sp. strain PCC 7942, nrtBCD encode membrane components of an ATP-binding cassette transporter involved in the transport of nitrite as well as of nitrate, whereas nrtA encodes a 45-kDa cytoplasmic membrane protein, the biochemical function of which remains unclear. Characterization of the nrtA deletional mutants showed that the 45-kDa protein is essential for the functioning of the nitrate/nitrite transporter. A truncated NrtA protein lacking the N-terminal 81 amino acids, expressed in Escherichia coli cells as a histidine-tagged soluble protein, was shown to bind nitrate and nitrite with high affinity (Kd = 0.3 microM). Immunoblotting analysis using the antibody against the 45-kDa protein revealed a 48-kDa precursor of the protein, which accumulated in the cyanobacterial cells treated with globomycin, an antibiotic that specifically inhibits cleavage of the signal peptide of lipoprotein precursors. These findings indicated that the nrtA gene product is a nitrate- and nitrite-binding lipoprotein. The N-terminal sequences of putative cyanobacterial substrate-binding proteins suggested that lipoprotein modification of substrate-binding proteins of ATP-binding cassette transporters is common in cyanobacteria. 相似文献
3.
Mixed oxide catalyst prepared by co-precipitating magnesium oxide and calcium oxide showed an excellent activity for the oxidative coupling of methane. The high performances were presumed to arise from the high basicity of the mixed oxide. 相似文献
4.
N,N'- Unsymmetrical dialkyl-3,4:9,10-perylenebis(dicarboximide)s (in which alkyl = methyl, ethyl, propyl, butyl, isobutyl, pentyl, hexyl and octyl) were prepared by the condensation of N-alkyl-3,4:9,10-perylene-tetracarboxylic monoanhydride monoimide with the appropriate alkyl-amines. The properties of these derivatives as pigments were tested and their thermal stability measured. 相似文献
5.
N′-Alkyl-N′-aryl-3,4:9,10-perylenebis(dicarboximide) (alkyl = isobutyl, pentyl, hexyl, octyl, etc.; aryl = phenyl, p-tolyl, p-methoxyphenyl, etc.) were prepared by the condensation of N-alkyl-3,4:9,10-perylene-tetracarboxylic monoanhydride monoimide with arylamines (aniline, p-toluidine, p-anisidine, o-phenylenediamine, etc.). The properties of these derivatives as pigments were tested, and also the thermal stability of 3,4:9,10-perylenebis(dicarboximide) derivatives was measured. 相似文献
6.
An ozone generator using a rotating electrode to improve ozone generation efficiency is proposed. The ozone generator electrode unit consists of a rotating electrode and fixed electrode. The rotating electrode has the grounded 36 pieces of tungsten wires fixed in parallel to the rotation axis on the rotating cylinder surface. A dielectric electrode is used as a fixed electrode located on the inside of the tube of the electrode unit. The width of the apparent discharge gap is 1mm. Alternating current with a frequency of 50 Hz is applied to the electrode unit. The rotation speed can be adjusted from 0 rpm to 1200 rpm by a variable speed motor. Oxygen gas is used as the material gas. Higher ozone concentration and higher ozone generation efficiency are obtained compared with that when the rotation speed is 0 rpm. The gas temperature is measured at the inlet and outlet of the ozone generator, and the rotation speed for the cooling effect is most effective at about 500 rpm. The maximum generation efficiency is estimated to be 61 g/kWh at 800 rpm, and this value is twice as large as in the case of 0 rpm. 相似文献
7.
S Kawai F Imazeki O Yokosuka M Ohto S Shiina N Kato M Omata 《Canadian Metallurgical Quarterly》1995,22(1):112-117
Analysis of X-chromosome inactivation patterns in women has been used to assess the clonality of various tumors. In this report, we analyzed 27 liver tumors in women, including 18 samples obtained by the performance of ultrasonically guided thin-needle biopsies. By analysis of the heterogeneity of phosphoglycerate kinase gene, 11 of 27 (41%) cases were found to be heterozygous at the gene. Of these informative 11 cases with liver tumors, 7 cases were "large" tumors (> 25 mm in diameter) and 4 cases were "small" tumors (< 25 mm in diameter). All 7 large tumors showed monoclonal patterns by the phosphoglycerate kinase gene analysis. Of the 4 small tumors, 2 showed monoclonal, and 2 showed polyclonal patterns. The 2 with monoclonal patterns were pathologically diagnosed as hepatocellular carcinoma despite their small sizes (20 mm and 23 mm). Of the two with polyclonal patterns, the smallest one (15 mm) was diagnosed as benign adenomatous hyperplasia, and the other as hepatocellular carcinoma heavily infiltrated by lymphocytes. These data suggest that analysis of the methylation pattern of the phosphoglycerate kinase gene may be helpful on rare occasions in elucidating the nature of liver tumors but must in fact be used in conjunction with histological appearances to avoid errors secondary to inflammatory infiltrates. 相似文献
8.
Development of flexible displays using back‐channel‐etched In–Sn–Zn–O thin‐film transistors and air‐stable inverted organic light‐emitting diodes 下载免费PDF全文
Mitsuru Nakata Genichi Motomura Yoshiki Nakajima Tatsuya Takei Hiroshi Tsuji Hirohiko Fukagawa Takahisa Shimizu Toshimitsu Tsuzuki Yoshihide Fujisaki Toshihiro Yamamoto 《Journal of the Society for Information Display》2016,24(1):3-11
We developed flexible displays using back‐channel‐etched In–Sn–Zn–O (ITZO) thin‐film transistors (TFTs) and air‐stable inverted organic light‐emitting diodes (iOLEDs). The TFTs fabricated on a polyimide film exhibited high mobility (32.9 cm2/Vs) and stability by utilization of a solution‐processed organic passivation layer. ITZO was also used as an electron injection layer (EIL) in the iOLEDs instead of conventional air‐sensitive materials. The iOLED with ITZO as an EIL exhibited higher efficiency and a lower driving voltage than that of conventional iOLEDs. Our approach of the simultaneous formation of ITZO film as both of a channel layer in TFTs and of an EIL in iOLEDs offers simple fabrication process. 相似文献
9.
Tetsuro Kobayashi Jeffrey Boase Tsutomu Suzuki Takahisa Suzuki 《Journal of Computer-Mediated Communication》2015,20(3):330-345
The tele‐cocooning hypothesis posits that mobile communication increases interaction with communication rich ties, while simultaneously weakening interaction with communication weak ties. In this study, we demonstrate how smartphones can be used to mitigate tele‐cocooning behavior by stimulating interaction with communication weak ties. Using a smartphone application to collect non‐identifying mobile communication log data, we conducted a field experiment with 193 Japanese participants. The treatment consisted of onscreen reminders designed to stimulate interaction with communication weak ties. The results indicate that the treatment promoted the activation of communication weak ties and the acquisition of information through those ties, suggesting that smartphones can be utilized to promote access to social capital. 相似文献
10.
Tajima T Takiguchi N Kato J Ikeda T Kuroda A Ohtake H 《Journal of Bioscience and Bioengineering》2003,96(2):149-153
The nematode Caenorhabditis elegans exhibits chemotaxis toward a wide variety of chemicals including water-soluble molecules and volatile organic compounds. We have previously discovered that C. elegans wild-type strain N2 is strongly attracted by cycloheximide which has long been known as a bitter tastant for humans and other mammals. We describe here the isolation and initial characterization of the first mutants which were defective specifically in their attraction to cycloheximide. In our screenings, we selected two mutants that were defective in chemotaxis to cycloheximide but normal in their attraction to NH4Cl and histidine. These mutants also avoided quinine hydrochloride, CuSO4 and high concentrations of NaCl similar to the wild-type strain N2. Furthermore, no observable defect was detected in their attraction to volatile odorants such as isoamyl alcohol and diacetyl. Dye-filling experiments suggested that they have no morphological defect in the sensory endings of the amphid neurons. 相似文献