‘Podracing’: experimenting with mobile TV content consumption and delivery methods

Recently, mobile TV has been launched in several countries. While mobile TV integrates television contents into mobile phones, the most personal of communication devices, it becomes interesting to know how this feature will be used throughout the day and in varying contexts of everyday life. This paper presents empirical results on the use of mobile TV with different delivery mechanisms and both quantitative and qualitative results on how end-users prefer to use mobile TV contents in different situations. The data is based on ongoing empirical research in Finland in 2006 and 2007. The mobile TV services under study included both news and entertainment contents, and were tested in 3G, DVB-H and Wi-Fi networks using different delivery paradigms: broadcast, on-demand and download. To explore the use of different delivery methods and content consumption, we have developed a mobile TV service protoype, called Podracing. The analysis shows that users appreciated up-to-date information and information-rich media forms and contents especially for mobile news delivery. There was high demand for only the latest news on mobiles. The real-time property was considered important. Most of the users looked at the headlines or followed the news several times a day – much more often than the traditional TV and news prime times would allow.     

Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells

The small intestinal epithelium has an important role in nutrition, but also in drug absorption and metabolism. There are a few two-dimensional (2D) patient-derived induced pluripotent stem cell (iPSC)-based intestinal models enabling easy evaluation of transcellular transport. It is known that animal-derived components induce variation in the experimental outcomes. Therefore, we aimed to refine the differentiation protocol by using animal-free components. More specifically, we compared maturation of 2D-cultured iPCSs toward small intestinal epithelial cells when cultured either with or without serum, and either on Geltrex or on animal-free, recombinant laminin-based substrata. Differentiation status was characterized by qPCR, immunofluorescence imaging, and functionality assays. Our data suggest that differentiation toward definitive endoderm is more efficient without serum. Both collagen- and recombinant laminin-based coating supported differentiation of definitive endoderm, posterior definitive endoderm, and small intestinal epithelial cells from iPS-cells equally well. Small intestinal epithelial cells differentiated on recombinant laminin exhibited slightly more enterocyte specific cellular functionality than cells differentiated on Geltrex. Our data suggest that functional small intestinal epithelial cells can be generated from iPSCs in serum-free method on xeno-free substrata. This method is easily converted to an entirely xeno-free method.     

Modulation of Aβ42 Aggregation Kinetics and Pathway by Low-Molecular-Weight Inhibitors

The aggregation of amyloid-β 42 (Aβ42) is directly related to the pathogenesis of Alzheimer's disease. Here, we have investigated the early stages of the aggregation process, during which most of the cytotoxic species are formed. Aβ42 aggregation kinetics, characterized by the quantification of Aβ42 monomer consumption, were tracked by real-time solution NMR spectroscopy (RT-NMR) allowing the impact that low-molecular-weight (LMW) inhibitors and modulators exert on the aggregation process to be analysed. Distinct differences in the Aβ42 kinetic profiles were apparent and were further investigated kinetically and structurally by using thioflavin T (ThT) and transmission electron microscopy (TEM), respectively. LMW inhibitors were shown to have a differential impact on early-state aggregation. Insight provided here could direct future therapeutic design based on kinetic profiling of the process of fibril formation.     

BET Inhibition Upregulates SIRT1 and Alleviates Inflammatory Responses

Control of histone acetylation is a part of the epigenetic mechanism that regulates gene expression and chromatin architecture. The members of the bromodomain and extra terminal domain (BET) protein family are a group of epigenetic readers that recognize histone acetylation, whereas histone deacetyl‐ ases such as sirtuin 1 (SIRT1) function as epigenetic erasers. We observed that BET inhibition by the specific inhibitor JQ1 upregulated SIRT1 expression and activated SIRT1. Moreover, we observed that BET inhibition functionally reversed the pro‐inflammatory effect of SIRT1 inhibition in a cellular lung disease model. SIRT1 activation is desirable in many age‐related, metabolic and inflammatory diseases; our results suggest that BET protein inhibition would be beneficial in treatment of those conditions. Most importantly, our findings demonstrate a novel mechanism of SIRT1 activation by inhibition of the BET proteins.     

Issues related to the computer realization of a multidisciplinary and multiobjective optimization system

Issues and novel ideas to be considered when developing computer realizations of complex multidisciplinary and multiobjective optimization systems are introduced. The aim is to discuss computer realizations that make possible both computationally efficient multidisciplinary analysis and multiobjective optimization of real world problems. We introduce software tools that make typically very time-consuming simulation processes more effective and, thus, enable even interactive multiobjective optimization with a real decision maker. In this paper, we first define a multidisciplinary and multiobjective optimization system and after that present an implementation overview of such problems including basic components participating in the solution process. Furthermore, interfaces and data flows between the components are described. A couple of important features related to the implementation are discussed in detail, for example, the usage of automatic differentiation. Finally, the ideas presented are illustrated with an industrial multiobjective optimization problem, when we describe numerical experiments related to quality properties in paper making.     

Bi-level optimization for a dynamic multiobjective problem

In this article, a bi-level optimization problem covering upper (design) and lower (operation) levels is defined and a solution procedure for bi-level optimization problems is presented. This is devised as a dynamic multiobjective optimization problem, i.e. the values of the control and state variables change over a predefined time horizon and several competing criteria are optimized simultaneously. Moreover, the interaction between the upper and lower levels is analysed. The benefits of bi-level dynamic multiobjective optimization are illustrated in detail by examining an industrial case in which the design of a paper mill (upper level) and the mill operation (lower level) are optimized at the same time. However, the problem definition and the solution procedure are not limited to any specific application but can be exploited in many different industrial areas.     

Multiobjective process line optimization under uncertainty applied to papermaking

Multiobjective optimization methodology for the development of the papermaking process is considered. The aim is to find efficient and reliable solution procedures for the process line model consisting of sequential unit-process models; some of them based on physics, whereas others on experimental data. By the consequence of modeling procedures, nonphysical states or inherited from modeling data in statistical case, the unit-process models may suffer from undesired unreliability. To control the uncertainty resulting from the unit-process models, a new multiobjective optimization approach is introduced where both the papermaking targets as well as the uncertainty related unit-process models are simultaneously taken into account. We illustrate the solution process by numerical examples related to the quality of the produced paper.