一种新型背面接触式H~+-ISFET

简要地介绍了氢离子敏场效应晶体管(H~+-ISFET)的基本结构和工作原理,提出了一种新型背面接触式H~+-ISFET。对该结构在版图、工艺设计中的有关问题,进行了探讨。最后指出:它在pH值的测量中有较好的实用价值。     

隔热铝型材的生产实践

本文介绍了穿条隔热铝合金型材在生产当中出现的问题，并对此进行了分析，促进隔热材的生产。     

Cyt-C Mediated Mitochondrial Pathway Plays an Important Role in Oocyte Apoptosis in Ricefield Eel (Monopterus albus)

Apoptosis plays a key role in the effective removal of excessive and defective germ cells, which is essential for sequential hermaphroditism and sex change in vertebrates. The ricefield eel, Monopterus albus is a protogynous hermaphroditic fish that undergoes a sequential sex change from female to male. Previous studies have demonstrated that apoptosis is involved in sex change in M. albus. However, the apoptotic signaling pathway is unclear. In the current study, we explored the underlying mechanism of apoptosis during gonadal development and focused on the role of the mitochondrial apoptosis signaling pathway in sex change in M. albus. Flow cytometry was performed to detect apoptosis in gonads at five sexual stages and ovary tissues exposed to hydrogen peroxide (H₂O₂) in vitro. Then the expression patterns of key genes and proteins in the mitochondrial pathway, death receptor pathway and endoplasmic reticulum (ER) pathway were examined. The results showed that the apoptosis rate was significantly increased in the early intersexual stage and then decreased with the natural sex change from female to male. Quantitative real-time PCR revealed that bax, tnfr1, and calpain were mainly expressed in the five stages. ELISA demonstrated that the relative content of cytochrome-c (cyt-c) in the mitochondrial pathway was significantly higher than that of caspase8 and caspase12, with a peak in the early intersexual stage, while the levels of caspase8 and caspase12 peaked in the late intersexual stage. Interestingly, the Pearson’s coefficient between cyt-c and the apoptosis rate was 0.705, which suggests that these factors are closely related during the gonadal development of M. albus. Furthermore, the cyt-c signal was found to be increased in the intersexual stage by immunohistochemistry. After incubation with H₂O₂, the mRNA expression of mitochondrial pathway molecules such as bax, apaf-1, and caspase3 increased in ovary tissues. In conclusion, the present results suggest that the mitochondrial apoptotic pathway may play a more important role than the other apoptotic pathways in sex change in M. albus.     

Expression Patterns and Gonadotropin Regulation of the TGF-β II Receptor (Bmpr2) during Ovarian Development in the Ricefield Eel Monopterus albus

Bmpr2 plays a central role in the regulation of reproductive development in mammals, but its role during ovarian development in fish is still unclear. To ascertain the function of bmpr2 in ovarian development in the ricefield eel, we isolated and characterized the bmpr2 cDNA sequence; the localization of Bmpr2 protein was determined by immunohistochemical staining; and the expression patterns of bmpr2 in ovarian tissue incubated with FSH and hCG in vitro were analyzed. The full-length bmpr2 cDNA was 3311 bp, with 1061 amino acids encoded. Compared to other tissues, bmpr2 was abundantly expressed in the ovary and highly expressed in the early yolk accumulation (EV) stages of the ovary. In addition, a positive signal for Bmpr2 was detected in the cytoplasm of oocytes in primary growth (PG) and EV stages. In vitro, the expression level of gdf9, the ligand of bmpr2, in the 10 ng/mL FSH treatment group was significantly higher after incubation for 4 h than after incubation for different durations. However, bmpr2 expression in the 10 ng/mL FSH treatment group at 2 h, 4 h and 10 h was significantly lower. Importantly, the expression level of bmpr2 and gdf9 in the 100 IU/mL hCG group had similar changes that were significantly decreased at 4 h and 10 h. In summary, Bmpr2 might play a pivotal role in ovarian growth in the ricefield eel, and these results provide a better understanding of the function of bmpr2 in ovarian development and the basic data for further exploration of the regulatory mechanism of gdf9 in oocyte development.     

在西文X Window环境下汉化用户程序的界面

如何在一个基本的西文ＸＷｉｎｄｏｗ环境下开发出自己的汉化产品，是很多Ｘ Ｗｉｎｄｏｗ用户正面临的一个现实问题。本文将给出一种汉化应用程序接口界面的简捷方法。程序员只需在编程时有关界面部分的Ｍｏｔｉｆ子程序稍作自发，即可达到界面汉化的目的。     

利用Autodesk Map实现资料的可视化管理

提出了利用地理信息系统的可视化思想来管理资料的方法,在此基础上以实例的方法展示了如何利用AutodeskMap软件建立图形化的资料管理系统.     

某锡铜硫化矿半自磨半工业试验研究

采用1 800×610 mm自磨机对云锡大屯选矿分公司处理的锡铜硫化矿进行半自磨半工业试验研究,通过对松树脚锡铜硫化矿进行磨机台时处理量、磨机转速、磨矿浓度、钢球充填率条件试验及连续稳定试验,结果表明,锡铜硫化矿采用半自磨工艺取代传统三段一闭路碎矿工艺是可行的,磨机排矿产品中新增-0.019 mm锡石过粉碎金属率可控制在较低的范围内。     

重要用途钢丝绳国家标准起草说明

介绍GB 8918—2006《重要用途钢丝绳》的制订过程和原则,并与GB/T 8918—1996,ISO 3154:1988,ISO2408:2002进行对比,指出在钢丝绳分类、材料、技术要求、直径公差、长度允差、拆股实验、镀锌层、伸长率等方面的异同,并对产生差别的原因进行说明。     

云南某高碳氧化铜矿浮选实验研究

对云南某地高碳氧化铜矿进行浮选实验研究,原矿含Cu 1.52％,氧化率48.06％,含Ag53.1g/t、C0.59％.采用脱碳-浮选和直接浮选对比实验,结果表明直接浮选工艺较合理.闭路实验获得了铜精矿产率4.24％,含Cu 33.46％,铜回收率为82.97％;含Ag 924 g/t、银回收率为73.78％的选别指标...     

浅谈EPC总承包模式下业主的工程质量管控措施

从业主的角度出发,介绍和探讨了EPC总承包模式下工程项目质量管理控制的思路及措施。