Giuseppe Gradenigo and his contributions to audiology

Giuseppe Gradenigo (1859-1926) was an important figure in the development of Otology. Within this paper the authors consider his background and his particular contributions to the development of various aspects of audiology.     

Enzymatic hydrolysis of cotton wastes. Effect of γ-ray pretreatments

Textile cotton wastes were irradiated with γ-rays at different doses and solubilized with 10% NaOH solutions. The regenerated products underwent enzymatic hydrolysis and their degradation pattern was compared with that of the cotton celluloses untreated or treated only with γ-rays. The different samples were characterized by measurements of X-ray diffraction, degree of polymerization, moisture regain and water retention. The structural and morphological modifications induced by the pretreatments were correlated with the variations of kinetic parameters, particularly the V_max/K_m ratio, defined as specificity constant. Only the combination of irradiation with dissolution pretreatments gave a considerable increase of hydrolysis reaction effectiveness.     

Ethyl oleate ozonide as an epoxidation tool of C60 and C70 fullerenes

When dissolved in ethyl oleate secondary ozonide, both C₆₀ and C₇₀ fullerenes undergo a series of epoxidation reactions. The pseudofirst-order kinetic rate constants of this process were determined spectrophotometrically at various temperatures and the activation energy for C₆₀ epoxidation through ethyl oleate ozonide was found at 25.9 kcal/mol. Furthermore, C₆₀ was found more reactive than C₇₀ with the ozonide. The kinetics rate constants of C₆₀ epoxidation with ethyl oleate ozonide were compared with the C₆₀ photo-oxidation and auto-oxidation determined in pure ethyl oleate. The epoxidation of fullerenes starts from the homolysis of the peroxide group of the 1,2,4-trioxolane ring of ethyl oleate secondary ozonide. Thus, it is suggested that fullerenes have a potential as decomposition agents of secondary ozonides in some technological applications.     

γ-Radiolysis of Polyynes in Heptane

The polyynes in heptane solution were γ-radiolyzed at a total dose of 150, 300 and 600 kGy. After radiolysis the products were analyzed by electronic absorption spectroscopy, by HPLC (liquid chromatography) and by FT-IR spectroscopy. Even at 150 kGy the polyynes disappear completely from the solution and the chromatographic analysis shows that the acetylenic chains undergo fragmentation, addition and crosslinking reactions with other chains and with solvent. The oxidative interference from air has been detected by FT-IR.     

Soot and other Products Formation from the Submerged Carbon Arc in Halogenated Solvents

A series of selected halomethanes were treated with the submerged carbon arc using graphite electrodes at 10A. CCl₄ and CHCl₃ produce abundant soot which is double in comparison to the soot produced by arcing CH₂Cl₂ and is one order of magnitude higher than the amount of soot produced by arcing hydrocarbons such as n-hexane or decalin and two orders of magnitudes higher in comparison to the amounts of soot produced by arcing alcohols. It was found a anticorrelation between the amount of polyynes present in the solution of the arced solvent and the amount of soot produced by arcing. In the case of CCl₄ and CHCl₃ no polyynes were detected by liquid chromatographic analysis (HPLC-DAD), while polyynes were detected in CH₂Cl₂ and are present in higher concentration in arced hydrocarbon solutions. The soot produced from halomethanes was analyzed by FT-IR spectroscopy in comparison to the soot produced by arcing hydrocarbons and alcohols. Furthermore, the soot from halomethanes was analyzed by HPLC-DAD after extraction with acetonitrile and found to contain a mixture of halogenated polycyclic aromatic hydrocarbons (PAHs). Also CHClBr and CH₂I₂ were arced between graphite electrodes. The former compound produces free bromine and soot; bromine causes halogenation of the polyynes. In the case of the latter compound, free iodine is released from the arc and the amount of soot produced is comparable to that obtained by arcing CH₂Cl₂.     

Genomic Analysis Made It Possible to Identify Gene-Driver Alterations Covering the Time Window between Diagnosis of Neuroblastoma 4S and the Progression to Stage 4

Neuroblastoma (NB) is a tumor of the developing sympathetic nervous system. Despite recent advances in understanding the complexity of NB, the mechanisms that determine its regression or progression are still largely unknown. Stage 4S NB is characterized by a favorable course of disease and often by spontaneous regression, while progression to true stage 4 is a very rare event. Here, we focused on genomic analysis of an NB case that progressed from stage 4S to stage 4 with a very poor outcome. Array-comparative genomic hybridization (a-CGH) on tumor-tissue DNA, and whole-exome sequencing (WES) on exosomes DNA derived from plasma collected at the onset and at the tumor progression, pointed out relevant genetic changes that can explain this clinical worsening. The combination of a-CGH and WES data allowed for the identification iof somatic copy number aberrations and single-nucleotide variants in genes known to be responsible for aggressive NB. KLRB1, MAPK3 and FANCA genes, which were lost at the time of progression, were studied for their possible role in this event by analyzing in silico the impact of their expression on the outcome of 786 NB patients.     

Toward optimization of a robust low-cost sulfonated-polyethersulfone containing layered double hydroxide for PEM fuel cells

Polyethersulphone (PES) is an aromatic thermoplastic, at low environmental impact, evaluated in this work as a promising candidate for new polymer electrolytes in the PEMFCs technology. A sulfonation procedure has been tuned in order to graft sulfonic acid groups on the polymer chains (sPES) and to make it hydrophilic. Homogeneous membranes with different polymer's sulfonation degrees (SD%) have demonstrated excellent mechanical properties and very low permeability toward methanol (important in the DMFCs), even if low proton conductivity. Nanocomposite sPES membranes were prepared by dispersion of highly hydrophilic lamellar particles such as layered double hydroxide (LDH) in the polymer. Deep investigations performed by a combination of PFG-NMR, EIS, XRD, DMA, and scanning electron microscopy have evidenced the exfoliation of the lamellae in polymer matrix. However, a certain anisotropy was evidenced both in the morphology and molecular diffusion, favored in the longitudinal direction (parallel to surface), while completely inhibited in the cross-section. This finding is most likely induced by the polymer structure, therefore particular attention must be paid to the choice of the filler and preparation of the composites. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47884.     

On the Isolated Pentagon Rule: Could Fullerenes Exist Violating This Rule?

Abstract

Through a short review on pentalene chemistry, it is discussed the possibility to trap as organometallic complexes unstable fullerenes violating the isolated pentagons rule.     

Uncertainty Estimation in Simultaneous Measurements of Levels and Permittivities of Liquids Using TDR Technique

Time-domain reflectometry (TDR) based on instruments are commonly used for several monitoring methods, particularly in soil moisture and volumetric water-content evaluation. Furthermore, significant advantages of TDR methodology, mostly related to the possible determination in real-time and to a nondestructive approach of the spatial location and nature of various objects, make this technique an appealing candidate for a variety of environmental and industrial applications. In this paper, we show that the suitable combination of TDR-detecting functionalities can lead to a joint quantitative and qualitative monitoring method for liquid-control purposes, so that, in one shot, the analysis of TDR data allow the measurement of liquid levels, the determination of multiple interfaces in layered media, as well as the evaluation of dielectric properties, thus opening challenging perspectives for several monitoring applications, particularly in fluid-processing-related industry. For such purposes, a detailed analysis of the uncertainty of the proposed measurement method is mandatory; hence, a metrological characterization of the method is carried out, demonstrating that the presented technique is definitely valid for simultaneously measuring levels and dielectric constants of liquids, with uncertainties under 2%. Results obtained for different liquid samples validate the approach on a wide range of dielectric materials and demonstrate the robustness and reliability of the proposed TDR technique.     

CMOS Non‐tailed differential pair

A continuous‐time complementary metal–oxide–semiconductor differential pair that does not require the traditional tail current source as a way to control the direct current and common‐mode current is presented. Compared with a p‐channel long‐tailed pair, the proposed non‐tailed solution operates under a higher maximum input common‐mode voltage that includes (V_DD + V_SS)/2 even under low supply voltages. Experimental measurements on a prototype fabricated in a 0.35‐µm technology (with metal – oxide – semiconductor thresholds greater than 0.6 V) confirm this behavior for supply voltages as low as 1.2 V, whereas the long‐tailed pair with the same technology offers the same capability only for supplies higher than 1.6 V. Copyright © 2015 John Wiley & Sons, Ltd.