Effect of aeration and agitation on growth rate of Thermus thermophilus in batch mode

The growth kinetics of Thermus thermophilus HB27 was investigated in rich medium (Thermus medium) under batch cultivation at 65 degrees C in 3-l fermentors. The growth and oxygen consumption rates were highly dependent on the aeration and agitation rates. Volumetric mass transfer coefficient (K(L)a, h(-1)) and hence oxygen transfer rate (OTR, mol m (-3) h(-1)) into the fermentation broth increased with increased aeration and/or agitation rates. A K(L)a and OTR of 175.4 h(-1) and 31.7 mol m(-3)h(-1) respectively, corresponding to 500 rpm agitation and 2 vvm aeration with a mixture of air and oxygen, were required to avoid oxygen limitation. The maximum growth rate (mu(max), h(-1)), doubling time (t(D), h), and dry cell weight determined for T. thermophilus HB27 growing under these conditions were 0.27 h(-1), 2.67 h, and 3g/l respectively. This cell yield is higher than any previously published reports for growth of Thermus cultures, including studies that employed pressurized bioreactors.     

The effect of internal pressure on a penny-shaped crack at the interface of two bonded dissimilar micropolar elastic half-spaces

In the linear theory of micropolar elasticity, the problem of a penny-shaped crack at the interface of two bonded dissimilar micropolar elastic half spaces is studied. The problem is first reduced to a system of dual integral equations which are further reduced to the solution of Riemann-Hilbert problem. Further stresses at the rims of cracks and in the vicinity have been evaluated.     

SSNET: an improved deep hybrid network for hyperspectral image classification

Neural Computing and Applications - Classification is one of the most important task in hyperspectral image processing. In the last few decades, several classification techniques have been...     

Enzymatic Synthesis of Magnetic Nanoparticles

We report the first in vitro enzymatic synthesis of paramagnetic and antiferromagnetic nanoparticles toward magnetic ELISA reporting. With our procedure, alkaline phosphatase catalyzes the dephosphorylation of l-ascorbic-2-phosphate, which then serves as a reducing agent for salts of iron, gadolinium, and holmium, forming magnetic precipitates of Fe_45±14Gd_5±2O_50±15 and Fe_42±4Ho_6±4O_52±5. The nanoparticles were found to be paramagnetic at 300 K and antiferromagnetic under 25 K. Although weakly magnetic at 300 K, the room-temperature magnetization of the nanoparticles found here is considerably greater than that of analogous chemically-synthesized Ln_xFe_yO_z (Ln = Gd, Ho) samples reported previously. At 5 K, the nanoparticles showed a significantly higher saturation magnetization of 45 and 30 emu/g for Fe_45±14Gd_5±2O_50±15 and Fe_42±4Ho_6±4O_52±5, respectively. Our approach of enzymatically synthesizing magnetic labels reduces the cost and avoids diffusional mass-transfer limitations associated with pre-synthesized magnetic reporter particles, while retaining the advantages of magnetic sensing.     

Dynamics and control of multiple cooperating manipulators with rolling contacts

In this article, we investigate the control of multiple cooperating mechanisms manipulating a common object, with rolling contact between the end-effectors and the object. We derive new kinematic and dynamic formulations for the system, in which the rolling degrees of freedom between the end-effector and the object are modeled as one or more unactuated joints of the manipulator. Differential geometric concepts are used to interpret the curvature and torsion forms used in contact kinematic equations derived by Montana. The augmentation of the rolling contacts imparts each manipulator with additional degrees of freedom and could also make it kinematically redundant. This can be exploited for the satisfaction of a secondary subtask criterion, such as collision avoidance, which is demonstrated in the examples. In addition, a control law can be specified that enables simultaneous control of the object trajectory, the internal forces in the object, and the trajectories of the contact points on the object or the end-effectors. © 1996 John Wiley & Sons, Inc.     

Tuning the Magnetic Properties of Nanoparticles

The tremendous interest in magnetic nanoparticles (MNPs) is reflected in published research that ranges from novel methods of synthesis of unique nanoparticle shapes and composite structures to a large number of MNP characterization techniques, and finally to their use in many biomedical and nanotechnology-based applications. The knowledge gained from this vast body of research can be made more useful if we organize the associated results to correlate key magnetic properties with the parameters that influence them. Tuning these properties of MNPs will allow us to tailor nanoparticles for specific applications, thus increasing their effectiveness. The complex magnetic behavior exhibited by MNPs is governed by many factors; these factors can either improve or adversely affect the desired magnetic properties. In this report, we have outlined a matrix of parameters that can be varied to tune the magnetic properties of nanoparticles. For practical utility, this review focuses on the effect of size, shape, composition, and shell-core structure on saturation magnetization, coercivity, blocking temperature, and relaxation time.