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排序方式: 共有215条查询结果,搜索用时 15 毫秒
1.
Miyahara Daiki Ueda Itaru Hayashi Yu-ichi Mizuki Takaaki Sone Hideaki 《International Journal of Information Security》2021,20(5):729-740
International Journal of Information Security - Card-based cryptography is an attractive and unconventional computation model; it provides secure computing methods using a deck of physical cards.... 相似文献
2.
Naoki Motomura Yuto Yamazaki Daiki Koga Shogo Harashima Xin Gao Yuta Tezuka Kei Omata Yoshikiyo Ono Ryo Morimoto Fumitoshi Satoh Yasuhiro Nakamura Go Eun Kwon Man Ho Choi Akihiro Ito Hironobu Sasano 《International journal of molecular sciences》2022,23(4)
Cortisol-producing adenoma (CPA) is composed of clear and compact cells. Clear cells are lipid abundant, and compact ones lipid poor but associated with higher production of steroid hormones. PRKACA mutation (PRKACA mt) in CPA patients was reported to be associated with more pronounced clinical manifestation of Cushing’s syndrome. In this study, we examined the association of histological features and genotypes with cholesterol uptake receptors and synthetic enzymes in 40 CPA cases, and with the quantitative results obtained by gas chromatography-mass spectrometry (GC-MS) analysis in 33 cases to explore their biological and clinical significance. Both cholesterol uptake receptors and synthetic enzymes were more abundant in compact cells. GC-MS analysis demonstrated that the percentage of compact cells was inversely correlated with the concentrations of cholesterol and cholesterol esters, and positively with the activity of cholesterol biosynthesis from cholesterol esters. In addition, hormone-sensitive lipase (HSL), which catalyzes cholesterol biosynthesis from cholesterol esters, tended to be more abundant in compact cells of PRKACA mt CPAs. These results demonstrated that both cholesterol uptake and biosynthesis were more pronounced in compact cells in CPA. In addition, more pronounced HSL expression in compact cells of PRKACA mt CPA could contribute to their more pronounced clinical manifestation. 相似文献
3.
Yoshinori Iwahashi Zenji Horita Minoru Nemoto Terence G. Langdon 《Metallurgical and Materials Transactions A》1998,29(10):2503-2510
Experiments were undertaken to compare the equal-channel angular (ECA) pressing of Al-1 pct Mg and Al-3 pct Mg solid-solution
alloys with pure Al. The results reveal both similarities and differences between these three materials. Bands of subgrains
are formed in all three materials in a single passage through the die, and these subgrains subsequently evolve, on further
pressings through the die, into an array of grains with high-angle boundaries. However, the addition of magnesium to an aluminum
matrix decreases the rate of recovery and this leads, with an increasing Mg content, both to an increase in the number of
pressings required to establish a homogeneous microstructure and to a decrease in the ultimate equiaxed equilibrium grain
size. It is concluded that alloys exhibiting low rates of recovery should be especially attractive candidate materials for
establishing ultrafine structures through grain refinement using the ECA pressing technique. 相似文献
4.
Yasutoshi Kasahara Masashi Kato Satoshi Watanabe Makio Iwahashi Rieko Wakamatsu Takashi Suzuki Akinori Kanetani Takaaki Kano Takamitsu Tamura 《Journal of the American Oil Chemists' Society》2012,89(7):1223-1229
Mineral oil is commonly used as an electrical insulating oil in transformers because of its relatively high electrical insulating
ability and fluidity. Considering the depletion of resources and environmental problems, however, fatty acid esters synthesized
from natural plant oils are attracting attention as an environmentally friendly insulating oil. In addition, fatty acid esters
such as methyl octanoate, methyl dodecanoate, 2-ethylhexyl octanoate, and 2-ethylhexyl dodecanoate have high fluidity, and
also show excellent moisture tolerance against dielectric breakdown compared to mineral oil. In the present study, to clarify
the reason for the superior moisture tolerance of fatty acid esters, the status of dissolved water in esters is investigated
with IR spectroscopic measurements and density functional theory (DFT) calculations. It is revealed that water molecules in
fatty acid esters are trapped by the ester moiety of fatty acid esters. As a result, fatty acid esters have a higher moisture
tolerance against dielectric breakdown than mineral oil. 相似文献
5.
6.
Maeda-Yamamoto M Saito T Nesumi A Tokuda Y Ema K Honma D Ogino A Monobe M Murakami A Murakami A Tachibana H 《Journal of the science of food and agriculture》2012,92(11):2379-2386
BACKGROUND: The purpose of this study was to evaluate the effects of leaf order or crop season on anthocyanins and other chemicals in the anthocyanin‐rich tea cultivar ‘Sunrouge’ (Camellia sinensis x C. taliensis) by using high‐performance liquid chromatography, and to study the effect of ‘Sunrouge’ extract on acetylcholinesterase (AChE) activity in human neuroblastoma SK‐N‐SH cells. RESULTS: The total anthocyanin content was higher in the third (3.09 mg g?1) than in the second (2.24 mg g?1) or first crop season (1.79 mg g?1). The amount of anthocyanins contained in the stem was high (1.61 mg g?1). In the third crop season, the concentrations of delphinidin‐3‐O‐β‐D ‐(6‐(E)‐p‐coumaroyl)galactopyranoside (DCGa), cyanidin‐3‐O‐β‐D ‐(6‐(E)‐p‐coumaroyl)galactopyranoside, delphinidin‐3‐O‐β‐D ‐galactopyranoside, delphinidin‐3‐O‐β‐D ‐(6‐O‐(Z)‐p‐coumaroyl)galactopyranoside, cyanidin‐3‐O‐β‐D ‐galactoside, and delphinidin‐3‐O‐β‐D ‐glucoside were 1.57 mg g?1, 0.52 mg g?1, 0.40 mg g?1, 0.22 mg g?1, 0.14 mg g?1, and 0.11 mg g?1, respectively. DCGa accounted for about 50% of the anthocyanins present. The suppressive effect of ‘Sunrouge’ water extract on AChE activity in human neuroblastoma SK‐N‐SH cells was the strongest among the three tea cultivars (‘Sunrouge’, ‘Yabukita’ and ‘Benifuuki’). CONCLUSION: These results suggested that ‘Sunrouge’ might protect humans from humans from AChE‐related diseases by suppressing AChE activity. To obtain sufficient amounts of anthocyanins, catechins and/or caffeine for a functional food material, ‘Sunrouge’ from the third crop season should be used. Copyright © 2012 Society of Chemical Industry 相似文献
7.
DNA-DNA hybridization is known as the superior method in the elucidation of relationships between closely related taxa, such as species and strain. For species determination we propose a new DNA-DNA hybridization method: the DNA microarray-based comparative genomic hybridization (CGH) method, using a yeast DNA microarray with approximately 6000 genes. The genome from a yeast strain as a sample strain (Sample) was labelled with Cy3-dye and hybridized to a single DNA microarray, together with the Cy5-labelled genome of S. cerevisiae S288C as a reference strain (Reference). The log2 ratio values [log2[Cy3(Sample)/Cy5(Reference)]: Ratio] of signal intensities of all the gene spots were estimated and divided into the following groups: Ratio < or = -1; -1 < Ratio < 1; 1 < or = Ratio. The hybridization profiles of the genomes of type strains belonging to the genus Saccharomyces were significantly different from that of S. cerevisiae S288C. The Ratio-based grouping allowed us to discriminate between some species from S. cerevisiae more clearly. Furthermore, cluster analysis discriminated between closely related species and strains. Using this method, we were able to not only perform species determination but also to obtain information on alternation in gene copy number of such gene amplifications and deletions with single-gene resolution. These observations indicated that DNA microarray-based CGH is a powerful system for species determination and comparative genome analysis. 相似文献
8.
Correlations between the chemical structures of agricultural fungicides and mRNA expression levels following exposure of Saccharomyces cerevisiae to toxic doses of thiuram, zineb, maneb, TPN, and PCP were examined. Structurally, thiuram, zineb, and maneb are dithiocarbamate fungicides, whereas TPN and PCP are not. To characterize chemical toxicity, genes expression was classified according to the functional groups used by the MIPS database. However, no correlations between the classification scheme and chemical structures were found. Hierarchical clustering of gene expression profiles was performed to characterize the effects of the five chemicals. According to this analysis the similarity of gene expression profiles depended on the similarity of chemical structures. These results suggest that DNA microarray technology has potential for predicting the major chemicals which will cause environmental toxicity and will provide information on new biomonitoring methods. 相似文献
9.
Daiki Uryu Tomohiro Tamaru Azusa Suzuki Rie Sakai 《Science and Technology of Advanced Materials》2016,17(1):691-697
The establishment and maintenance of axonal patterning is crucial for neuronal function. To identify the molecular systems that operate locally to control axonal structure, it is important to manipulate molecular functions in restricted subcellular areas for a long period of time. Microfluidic devices can be powerful tools for such purposes. In this study, we demonstrate the application of a microfluidic device to clarify the function of local Ca2+ signals in axons. Membrane depolarization significantly induced axonal branch-extension in cultured cerebellar granule neurons (CGNs). Local application of nifedipine using a polydimethylsiloxane (PDMS)-based microfluidic device demonstrated that Ca2+ entry from the axonal region via L-type voltage-dependent calcium channels (L-VDCC) is required for branch extension. Furthermore, we developed a method for locally controlling protein levels by combining genetic techniques and use of a microfluidic culture system. A vector for enhanced green fluorescent protein (EGFP) fused to a destabilizing domain derived from E. coli dihydrofolate reductase (ecDHFR) is introduced in neurons by electroporation. By local application of the DHFR ligand, trimethoprim (TMP) using a microfluidic device, we were able to manipulate differentially the level of fusion protein between axons and somatodendrites. The present study revealed the effectiveness of microfluidic devices to address fundamental biological issues at subcellular levels, and the possibility of their development in combination with molecular techniques. 相似文献
10.