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Hande DAGCI ?zgür KURT Mete DEMIREL Aliye MANDIRACIOGLU S?hret AYDEMIR Ulas SAZ Aldert BART Tom VAN GOOL 《Iranian Journal of Parasitology》2014,9(4):519-529
Background
The aims of this study were to identify Blastocystis subtypes (STs) in a cohort of Turkish patients with various gastrointestinal symptoms using a novel Real Time PCR method developed recently for Blastocystis detection and assess the relationship between Blastocystis STs and patient symptoms.Methods
Totally, 617 stool samples of patients with gastrointestinal symptoms were examined with microscopy and inoculated in Jones medium. Blastocystis-positive samples were further assessed to identify coinfections with other possible pathogens, including bacteria and viruses. Diagnostic efficacies of microscopy, culture and Real-Time PCR were compared. PCR products were sequenced to identify the subtypes of Blastocystis isolates.Results
Totally 94 (15.24%) samples were positive for Blastocystis after all methods. Among these, 83 of 94 (88.3%) samples were identified with all methods, while 11 were positive only with Real Time PCR. Diarrhea and abdominal pain were the leading symptoms in the patients. The only pathogenic agent identified in 76 of 94 (80.9%) patients was Blastocystis. Subtype 3 was the leading Blastocystis subtype (44.6%), while subtypes 6 and 7 were firstly isolated from symptomatic patients in our region.Conclusion
Comparison of three diagnostic methods indicated Real Time PCR as the most sensitive and specific method. Blastocystis was the only pathogenic agent among symptomatic patients, with subtype 3 being predominant. Patients with subtypes 6 and 7 need further assessments concerning the zoonotic potential of Blastocystis. 相似文献6.
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A. LARSSON B. MATTSSON E. A. K. WAUTERS J. D. VAN GOOL M. DURAN S. K. WADMAN 《Acta paediatrica (Oslo, Norway : 1992)》1981,70(3):301-308
ABSTRACT. Larsson, A., Mattsson, B., Wauters, E. A. K., van Gool, J. D., Duran, M. and adman, S. K. (Department of Paediatrics, Karolinska Institute, St. Goran's Children's Hospital, Stockholm, Sweden, and University Children's Hospital, "Het Wilhelmina Kinderziekenhuis", Utrecht, The Netherlands). 5-Oxoprolinuria due to 5-oxoproIinase deficiency in two brothers–a new inborn error of the γ-glutamyl cycle. Acta Paediatr Scand, 70:301, 1981.–Two brothers, aged 16 and 11 years, had recurrent episodes of vomiting, diarrhoea and abdominal pain, starting in infancy. In spite of extensive investigations no cause of their enterocolitis could be established. After several years symptomatic treatment was discontinued without any recurrence of symptoms. Their father and several paternal relatives have had kidney stones. Both boys developed urolithiasis and an oxalate-containing stone was removed from the elder brother's kidney. He had no hypercalciuria. His glomerular and tubular function tests were normal. Gas chromatography of urine from both brothers revealed massive excretion of L-5-oxoproline (pyroglutamic acid). Glutathione levels in erythrocytes of both patients were normal. The activities of enzymes of the γ-glutamyl cycle were analysed in erythrocytes, leukocytes and cultured skin fibroblasts. The level of glutathione synthetase was normal, as was the affinity of this enzyme for its substrate γ-glutamyl-cysteine. Feedback inhibition of γ-glutamyl-cysteine synthetase by glutathione was also normal. Both patients had a specific deficiency of 5-oxoprolinase, the activity of which was 2-A % of that of control subjects. Their parents had intermediate 5-oxoprolinase activities in fibroblasts, indicating a recessive mode of inheritance. Thus, 5-oxoprolinuria in these two patients was due to a lack of S-oxoprolinase, i.e., a new inborn error in the γ-glutamyl cycle. 相似文献
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Growth regulation and transcriptional activities of estrogen and progesterone in human endometrial cancer cells 总被引:1,自引:0,他引:1