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Objective To explore the effect of Fufangkushen on gastric cancer cell killing by human γδT cells. Methods Isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells in vitro. Fufangkushen at various concentrations was used to induce γδT cells and gastric cancer cell lines SGC-7901 for 24 hours, MTr assays was used to detect inhibitory effect of Fufangkushen on these cell lines, LDH assays was used to measure the cytotoxic activity of γδT cells, and flow cytometry was used to detect apoptosis of γδT cells and SGC-7901 before and after the treatment. Results Ten days after cultivation, proliferation ra-tio of γδT cells increased from 4.21% to 70.35% and CD44 was up to 94.0%. Inhibitory rate of Fufangkush-en on SGC-7901 at various concentrations was significantly higher than that on γδT cells (22.3% vs-22.4%, P<0.05). The negative inhibitory ratio on γδT cells showed a dose-dependent manner with Fufangkushen's concentrations ranging from 1/5 to 1/400. γδT cells cytotoxic activity to SGC-7901 induced by Fufangkushen for 24 h was higher than control group, (83.6% vs 71.2%, P<0.05). Apoptotic rate was significantly lower in γδT cells than in SGC-7901 (4.64% vs49.23%, P<0.05). Conclusion Fufangkushen, within routine concentration ranges, can promote γδT cells' proliferation, inhibit tumor cell growth and enhance γδT cells' cytotoxic activity. This may be beneficial to tumor adoptive immunotherapy and provide evidence for the appli-cation of Fufangkushen in the treatment of tumors. 相似文献
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目的探讨围生医学中手术麻醉对分娩时母婴免疫功能的影响。方法正常初产妇分为自然分娩(NL)组、择期手术分娩麻醉(PCS)组、急诊手术分娩麻醉(ECS)组,每组20例,用流式细胞术检测分娩时母体外周血和婴儿脐带衄巾B细胞、T淋巴细胞、NK细胞、NKT细胞、γδT细胞的百分比并进行比较。结果母体血中ECS组的B细胞和NKT细胞明显高于NL组、PCS组;婴儿脐血中PCS组的B细胞、NK细胞和T淋巴细胞CD4^+/CD8^+明显低于NC组和ECS组(P〈0.05),γδT细胞显著高于NL组和ECS组(P〈0.01)。结论急诊手术分娩对母体免疫方面的改变与自身应激反应有关,不影响婴儿的细胞免疫状态;择期手术分娩影响婴儿免疫状态。 相似文献
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目的探讨舒林酸提高γδT细胞杀伤胃癌细胞的作用机制。方法常规方法培养γδT细胞,收集培养第9天的γδT细胞用不同浓度的舒林酸(1、2、4、8、16、32μmol/L)诱导24h,收集培养上清液用于细胞因子γ-干扰素(IFN-γ)、肿瘤坏死因子α(TNF—α)和白细胞介素12(IL-12)检测,沉淀细胞用于穿孔素、粒酶B、NKG2D流式细胞测定和用乳酸脱氢酶(LDH)释放法检测γδT细胞杀伤胃癌细胞株(SGC-7901和BCG-823)活性。每组测试样本和指标均设未加药物诱导的对照组。结果舒林酸浓度在8μmol/L时,γδT细胞穿孔素和粒酶B达最高[分别为(71.5±5.3)%和(78.3±7.1)%],明显高于对照组[分别为(51.4±7.3)%和(60.9±7.1)%]。γδT细胞经不同浓度的舒林酸诱导后培养上清液中IL—12和TNF-α浓度没有差异;舒林酸在8μmol/L时上清液中IFN-γ浓度达最高[(246.1±20.7)ng/L],明显高于对照组[(196.1±13.2)ng/L];当舒林酸浓度达32μmol/L时,γδT细胞分泌的3种细胞因子明显低于对照组,两者比较有显著性差异(P〈0.05)。γδT细胞经舒林酸诱导后杀伤SGC-7901和BCG-823细胞活性在8μmol/L时达到峰值,此时对SGC-7901和BCG-823的杀伤活性分别为(73.6±3.9)%和(76.3±3.9)%,与对照组[(60.1±3.7)%和(59.2±4.5)%]比较有明显差异(P〈0.05)。结论舒林酸诱导后γδT细胞杀伤SGC-7901和BCG-823活性明显增高的机制可能与γδT细胞表达穿孔素和粒酶B及分泌的IFN-γ有关。 相似文献
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Objective To explore the effect of Fufangkushen on gastric cancer cell killing by human γδT cells. Methods Isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells in vitro. Fufangkushen at various concentrations was used to induce γδT cells and gastric cancer cell lines SGC-7901 for 24 hours, MTr assays was used to detect inhibitory effect of Fufangkushen on these cell lines, LDH assays was used to measure the cytotoxic activity of γδT cells, and flow cytometry was used to detect apoptosis of γδT cells and SGC-7901 before and after the treatment. Results Ten days after cultivation, proliferation ra-tio of γδT cells increased from 4.21% to 70.35% and CD44 was up to 94.0%. Inhibitory rate of Fufangkush-en on SGC-7901 at various concentrations was significantly higher than that on γδT cells (22.3% vs-22.4%, P<0.05). The negative inhibitory ratio on γδT cells showed a dose-dependent manner with Fufangkushen's concentrations ranging from 1/5 to 1/400. γδT cells cytotoxic activity to SGC-7901 induced by Fufangkushen for 24 h was higher than control group, (83.6% vs 71.2%, P<0.05). Apoptotic rate was significantly lower in γδT cells than in SGC-7901 (4.64% vs49.23%, P<0.05). Conclusion Fufangkushen, within routine concentration ranges, can promote γδT cells' proliferation, inhibit tumor cell growth and enhance γδT cells' cytotoxic activity. This may be beneficial to tumor adoptive immunotherapy and provide evidence for the appli-cation of Fufangkushen in the treatment of tumors. 相似文献
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目的探讨手术分娩时机对母、婴血中CD4+CD25+CD127low调节性T细胞(Treg)的影响。方法正常初产妇60例,按分娩方式分为阴道分娩(VD)、择期手术分娩(PCS)、急诊手术分娩(ECS)三组,每组20例,用流式细胞仪检测分娩时母体外周血和新生儿脐血T细胞中Treg百分数。结果新生儿脐血中Treg三组间比较差异无统计学意义(P>0.05),但PCS组明显高于VD组(P<0.05);母体外周血中Treg,三组中ECS组>PCS组>VD组,ECS组显著性高于VD组(P<0.01);三组母体、新生儿血中Treg均无相关性(P>0.05)。结论 PCS时新生儿血中Treg增高,可能会对婴儿免疫系统发育有一定的影响;分娩过程异常的ECS母体血中Treg增高可能与产妇免疫失衡有关。 相似文献