温针灸和电针治疗脾虚型单纯性肥胖症的对照研究

目的:比较温针灸与电针治疗脾虚型单纯性肥胖症的疗效.方法:将68例脾虚型单纯性肥胖症(包括脾虚湿阻、肺脾气虚、脾肾阳虚3型)患者随机分成温针灸组(36例)、电针组(32例).两组取穴均以中脘、水分、气海、中极、天枢、水道等为主,对其疗效及体重指标进行观察.结果:温针灸组总有效率为88.9%,电针组为71.9%,两组比较差异有显著性意义(P<0.05);温针灸组与电针组肥胖体重下降差异亦有显著性意义(P<0.05).结论:温针灸治疗脾虚单纯性肥胖疗效确切且持久,值得推广.     

衰老相关动物模型的应用进展

随着人口老龄化成为全世界面临的共同问题,衰老的研究也日益成为焦点.衰老模型是在衰老相关研究中必然涉及的问题,目前可选择的衰老模型有多种,如自然衰老模型、D-半乳糖衰老模型、快速衰老小鼠等,涉及多种组织器官的衰老.选择恰当的衰老模型对研究衰老机理、筛选抗衰老确切疗法、选择延缓衰老的特效药物有事半功倍的作用.     

Influence of moxa smoke on mitochondrial transmembrane potential and Bax/Bcl-2 in alveolar type II epithelial A549 cells

Objective

To investigate the influence of moxibustion products on mitochondrial transmembrane potential (MTP) and mRNA expression of Bax/Bcl-2 in alveolar type II epithelial A549 cells, and to further explore influence of moxibustion products on the oxidative damage of A549 cells.

Methods

Smoke and particles generated by moxibustion were collected using the filter box for gas sampling. The moxa smoke extract (MSE) was diluted sequentially to the final concentrations of 0.05 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL using the cell culture medium, and A549 cells were then intervened by the above MSE solution. Cell MTP was detected by JC-1 staining. Fluorescence quantitative polymerase chain reaction (PCR) was used to detect Bax/Bcl-2 mRNA expression of A549 cells.

Results

Compared with cells in the normal control group, MTP was significantly decreased in cells of 0.3 mg/mL and 0.4 mg/mL MSE intervention groups (P<0.01); while MTP showed no significant changes in cells of 0.05 mg/mL, 0.1 mg/mL and 0.2 mg/mL MSE intervention groups (P>0.05); compared with cells in 0.05 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.1 mg/mL, 0.2 mg/mL, 0.3 mg/mL and 0.4 mg/mL MSE intervention groups (P<0.05 ); compared with cells in 0.1 mg/mL MSE intervention group, MTP was decreased significantly in cells of 0.4 mg/mL MSE intervention group (P<0.01). Bax mRNA expression of cells in each concentration of MSE intervention group all showed no significant difference compared to that in the normal control group; Bcl-2 mRNA expression of cells was reduced with the increase of MSE intervention concentration. Wherein, Bcl-2 mRNA expressions of cells in 0.4 mg/mL and 0.3 mg/mL MSE intervention groups were significantly reduced compared with that of cells in the normal control group (P<0.05); Bcl-2 mRNA expression of cells in 0.4 mg/mL MSE intervention group was significantly reduced compared to that in 0.05 mg/mL MSE intervention group (P<0.05).

Conclusion

Certain higher concentration of moxa smoke could reduce MTP and mRNA expression of the anti-apoptosis gene Bcl-2 in alveolar type II epithelial A549 cells. Oxidative damage may be the important mechanism of apoptosis caused by the high concentration of moxa smoke solution, and further studies are necessary on the specific mechanisms.

     

弥漫增殖型糖尿病肾病伴缺血性肾损伤病案

1病历摘要 林某某,男,40岁.主因"双下肢水肿4个月"于2010年5月19日入院.患者4个月前无明显诱因出现双下肢水肿,尿量基本正常,无其他不适,就诊于某医院,查血压(BP) 180/110mmHg.尿常规:尿蛋白(PRO)(++++)、红细胞(RBC) 2～3/HP;生化:血糖( GLU) 9.3mmol/L、血肌酐(SCr)140μmol/L、尿素氮(BUN)8.97mmol/L、血尿酸(UA)477μmol/L、白蛋白(Alb) 17.3g/L、总胆固醇(TC) 7.87mmol/L、甘油三酯(TG) 2.87mmol/L、低密度脂蛋白胆固醇(LDL-C) 5.62mmol/L.诊断为慢性肾炎、肾病综合征、慢性肾功能衰竭.予缬沙坦、非洛地平、别嘌呤醇、碳酸氢钠片等口服,血压维持在140～160/70～80mmHg,但患者尿量逐渐减少,水肿加重,为求中医治疗转诊入我院.入院时症见:双下肢浮肿明显,腰酸,乏力,腹胀,怕冷,24小时尿量约1 300ml,夜尿3次,大便正常.     

以学科教学知识能力促进中医院校教师专业发展

从学科教学知识能力（Pedagogical content knowledge,PCK）的基本概念出发,以开展PCK提升的外部环境、促进教师自主发展和教学观念转变、教师发展价值取向改变等维度阐述PCK对中医院校教师专业发展的重要性,并从高等中医药教育的教师专业发展实际需求出发提出相应的对策。     

针灸治疗肿瘤化疗后骨髓抑制的最新研究进展

在肿瘤放化疗过程中,大多数患者会出现骨髓抑制。骨髓抑制会导致一系列病理改变,临床上常表现为严重的出血和感染,是阻碍肿瘤治疗的原因之一。已有研究表明针灸对化疗后骨髓抑制有较好的治疗作用。现通过综述针灸治疗肿瘤化疗后骨髓抑制相关的研究结果,从骨髓细胞、造血微环境及信号通路3个方面分析针灸治疗骨髓抑制的作用机制,以期为临床提供参考。     

郑氏“颈四步”手法治疗神经根型颈椎病疗效研究

目的:探究郑氏“颈四步”手法治疗神经根型颈椎病(CSR)的临床疗效,以及对患者临床症状、颈部生物学相关参数的影响。方法:选取60例CSR患者,按照随机数字表法分为观察组和对照组,每组各30例。观察组予郑氏“颈四步”手法治疗,对照组予一般推拿治疗,比较两组治疗总有效率、疼痛改善情况、颈功能活动度和椎间孔内臂丛神经根内径。结果:观察组治疗总有效率为86.67%,高于对照组的63.33%,差异有统计学意义(P<0.05)。治疗后,两组颈痛量表(NPQ)、视觉模拟评分(VAS)均降低,左旋转、右旋转、左侧屈和右侧屈等颈功能活动度明显改善,C_5～7神经根内径明显减小,且观察组的NPQ、VAS评分低于对照组(均P<0.05),各方向颈功能活动度大于对照组(均P<0.05),C_5～7神经根内径小于对照组(P<0.05)。结论:郑氏“颈四步”手法治疗CSR疗效较好,可有效缓解患者颈肩疼痛,改善颈椎活动度,缓解神经根水肿。     

外泌体源性的微小RNA在肝癌中的研究进展

肝癌是常见的恶性消化道肿瘤,外泌体是由各种体液分泌的膜性囊泡,其中包含有脂质、核酸、微小RNA、长链非编码RNA等多种物质.外泌体包裹下的miRNA拥有更好的生物稳定性.研究发现肿瘤细胞较正常细胞能分泌更多的外泌体,肿瘤细胞来源的外泌体中miRNA在肿瘤的进展、转移、耐药等多个方面起重要作用.本文阐述了外泌体源性的mi...     

红景天抗衰老作用研究概况

红景天为帚天科植物高山或西藏红景天的干燥根或根茎,具有延缓机体衰老,防止老年疾病等显著功效．其成分红景天甙、红景天素及茎叶提取物等主要通过促进细胞生长增殖．促进蛋白质合成,提高SOD和GSH-Px活性,降低LPO含量,降低体内自由基对细胞的氧化损害,以厦促进生长激素分泌,影响机体脂质代谢等方面发挥抗衰老作用。