A new family of yeast genes implicated in ergosterol synthesis is related to the human oxysterol binding protein

We have identified three yeast genes, KES1, HES1 and OSH1, whose products show homology to the human oxysterol binding protein (OSBP). Mutations in these genes resulted in pleiotropic sterol-related phenotypes. These include tryptophan-transport defects and nystatin resistance, shown by double and triple mutants. In addition, mutant combinations showed small but apparently cumulative reductions in membrane ergosterol levels. The three yeast genes are also functionally related as overexpression of HES1 or KES1 alleviated the tryptophan-transport defect in kes1Δ or osh1Δ mutants, respectively. Our study implicates this new yeast gene family in ergosterol synthesis and provides comparative evidence of a role for human OSBP in cholesterol synthesis.     

Calretinin-immunoreactive neurons in the human thalamus

To identify plant defense components that are important in restricting the growth of virulent pathogens, we screened for Arabidopsis mutants in the accession Columbia (carrying the transgene BGL2-GUS) that display enhanced disease susceptibility to the virulent bacterial pathogen Pseudomonas syringae pv. maculicola (Psm) ES4326. Among six (out of a total of 11 isolated) enhanced disease susceptibility (eds) mutants that were studied in detail, we identified one allele of the previously described npr1/nim1/sai1 mutation, which is affected in mounting a systemic acquired resistance response, one allele of the previously identified EDS5 gene, and four EDS genes that have not been previously described. The six eds mutants studied in detail (npr1-4, eds5-2, eds10-1, eds11-1, eds12-1, and eds13-1) displayed different patterns of enhanced susceptibility to a variety of phytopathogenic bacteria and to the obligate biotrophic fungal pathogen Erysiphe orontii, suggesting that particular EDS genes have pathogen-specific roles in conferring resistance. All six eds mutants retained the ability to mount a hypersensitive response and to restrict the growth of the avirulent strain Psm ES4326/avrRpt2. With the exception of npr1-4, the mutants were able to initiate a systemic acquired resistance (SAR) response, although enhanced growth of Psm ES4326 was still detectable in leaves of SAR-induced plants. The data presented here indicate that eds genes define a variety of components involved in limiting pathogen growth, that many additional EDS genes remain to be discovered, and that direct screens for mutants with altered susceptibility to pathogens are helpful in the dissection of complex pathogen response pathways in plants.     

Computational methods for determining the latest starting times and floats of tasks in interval-valued activity networks

In project management, three quantities are often used by project managers: the earliest starting date, the latest starting date and the float of tasks. These quantities are computed by the Program Evaluation and Review Techniques/Critical Path Method (PERT/CPM) algorithm. When task durations are ill known, as is often the case at the beginning of a project, they can be modeled by means of intervals, representing the possible values of these task durations. With such a representation, the earliest starting dates, the latest starting dates and the floats are also intervals. The purpose of this paper is to give efficient algorithms for their computation. After recalling the classical PERT/CPM problem, we present several properties of the concerned quantities in the interval-valued case, showing that the standard criticality analysis collapses. We propose an efficient algorithm based on path enumeration to compute optimal intervals for latest starting times and floats in the general case, and a simpler polynomial algorithm in the case of series-parallel activity networks.     

Prediction of stationary interfaces in coextrusion flows

A computation method for the development of the interface in coextrusion flows is proposed. It is based on the transformation of the nonmixing condition at the interface into a transport equation of the rheological constants in the whole domain. The coextrusion flow of a Newtonian fluid and of a Carreau fluid is tested. It is pointed out that the interface location is strongly dependent on the flow rate and on the flow rate ratio.     

MODELING VACUUM-CONTACT DRYING OF WOOD: THE WATER POTENTIAL APPROACH

ABSTRACT

A two-dimensional mathematical model for vacuum-contact drying of wood is presented. The moisture and heat equations are based on the water potential concept whereas the pressure equation is formulated considering unsteady state conservation equation of dry air. Most of the model parameters were determined during independent experiments. The set of equations is then solved in a coupled form using the finite element method. The validation of the model is performed using experimental results obtained during vacuum-contact drying of sugar maple sapwood. The experimental and calculated data are in good agreement. Nevertheless, some discrepancies are observed which can be attributed to the boundary conditions used and to the fact that heat transfer by convection was neglected.     

Optimized transmission line matrix model implementation for graphics processing units computing in built-up environment

The increase in computational capabilities has made time-domain methods applicable for long-range sound propagation modelling. However, such approaches remain very demanding in terms of computational resources. Most current computers are supplied with a powerful device which is still little exploited: the graphics processing unit (GPU). The paper describes an implementation of a transmission line matrix model which allows parallel calculations on heterogeneous systems. A voxelization algorithm used to generate the computational domain is presented. A splitting process is also expounded which makes feasible performing huge domains simulations by accurately dividing the computational domain into subdomains. Each subdomain is enlarged by introducing extra cells containing neighbours subdomains data in order to run several computational iterations on a graphic device without data exchange with the system memory. The influence of the ghost layer depth and the speeding up of computation time with GPU are then illustrated in a realistic built-up area.     

The osteoblastic phenotype in calcium-depleted and calcium-repleted rats: a structural and histomorphometric study

In a previous report we showed that young rats fed a calcium-free diet for 28 days developed severe hypocalcaemia and showed a significant increase in serum alkaline phosphatase activity. The main histological and cytochemical changes exhibited by these animals in bone of the metaphyseal primary spongiosa were: (1) hyperplasia of osteoblasts, (2) an increase in the frequency of tartrate-resistant acid phosphatase (TRAP)-positive osteoblasts apposed to osteoid, and (3) an excessive amount of osteoid tissue. In addition to typical osteoblasts, there was a subpopulation of osteoblast-like cells with coated pits, lysosome-like bodies and large cytoplasmic processes. In the present study, we investigated how the above parameters change when calcium-depleted rats are placed on a normal diet for 7 days. Such a regimen normalized calcium concentration and alkaline phosphatase activity in the serum. The osteoid thickness returned to normal and, in some areas, was fully calcified. Most osteoblasts no longer showed TRAP activity and their ultrastructure was similar to that found in controls. Despite an intense alkaline phosphatase activity, some of them still exhibited a number of macrophagic characteristics. They were TRAP-positive, and showed electron-dense bodies in the cytoplasm facing bone, an abundance of coated pits, calcified spicules impinging on the cell membrane and large processes extending into the mineralized matrix. We concluded that calcium deficiency causes hyperplasia of osteoblasts in primary spongiosa and an increase in expression of TRAP. It also induces changes in their phenotype characterized by the acquisition of macrophagic cellular features. While TRAP activity is normalized by calcium repletion, macrophagic characteristics persist. These results suggest that the osteoblast can modulate its phenotype according to its physiological status.     

Wind turbine performance under icing conditions

The wind energy market is in full growth in Quebec but technical difficulties due to cold climate conditions have occurred for most of the existing projects. Thus, icing simulations were carried out on a 0.2 m NACA 63 415 blade profile in the refrigerated wind tunnel of the Anti‐icing Materials International Laboratory (AMIL). The shapes and masses of the ice deposits were measured, as well as the lift and drag forces of the iced profiles. Scaling was carried out based on the 1.8 MW–Vestas V80 wind turbine technical data, for three different radial positions and two in‐fog icing conditions measured at the Murdochville wind farm in the Gaspé Peninsula. For both icing events, the mass of ice accumulated on the blade profile increased with an increase in the radial position. In wet regime testing (first icing event), glaze formed mostly near the leading edge and on the pressure side. It also accumulated by run‐off on the trailing edge of the outer half of the blade. In dry‐regime testing (second icing event), rime mostly accreted on the leading edge and formed horns. For both icing events, when glaze or rime accreted on the blade profile, lift decreased and drag increased. A load calculation using the blade element theory shows that drag force on the entire blade becomes too large compared to lift, leading to a negative torque and the stop of the wind turbine. Torque reduction is more significant on the outer third of the blade. Setting up a de‐icing system only on the outer part of the blade would enable significant decrease of heating energy costs. Copyright © 2007 John Wiley & Sons, Ltd.     

Mixed-interpolated elements for Reissner–Mindlin plates

We present in this paper a procedure to establish Reissner–Mindlin plate bending elements. The procedure is based on the idea to combine known results on the approximation of Stokes problems with known results on the approximation of elliptic problems. The proposed elements satisfy the mathematical conditions of stability and convergence, and some of them promise to provide efficient elements for practical solutions.     

Modeling of Moisture Behavior of Wood Planks in Nonvented Flat Roofs

A computer model was extended and adapted to simulate the hygrothermal behavior of building envelope-wood components. The model was used to predict moisture movement in wood planks forming the decks of nonvented flat roofs insulated with cellulose. The gradient of water potential was considered as the driving force for moisture movement in wood. The model required the determination of convective heat- and mass-transfer coefficients, the sorption curves, the effective water conductivity for different wood species, and the hygrothermal conditions within the assembly to characterize the mass-conservation equation. Once these parameters were integrated in the computer model, this approach was then validated by carrying a simulation of the drying process of wood planks using experimental data from a large-scale test.