Bioeffects of Rare Earth Complex on Rats Studied by NMR and ICP-MS Spectroscopy

Thedevelopmentofhigh fieldNMRspectrome terswithincreasedsensitivity,resolution,dynamic rangeandimprovedcomputingcapabilitieshasmade itpossibletouseNMRtechniqueforrapidmulticom ponentanalysisoflowMWcompoundsinbiofluids inordertoassessthetoxicologicaleffe…     

Investigation on Acute Biochemical Effects of Ce（NO3 ）3 on Liver and Kidney Tissues by MAS 1H NMR Spectroscopic-Based Metabonomic Approach

High resolution1Hmagicanglespinning(MAS)NMRspectroscopyisincreasinglybeingusedasause fultechniqueforinvestigatingthemetabolicstateof variousintacttissuesintheareasofdrugtoxicity[1,2]anddiseasediagnosis[3,4].Biologicaltissuesaresemi solidheterogeneouscomplexcontainingspecieswitha widerangeofmolecularweightssuchasproteins,lip ids,andlow molecularweightmetabolites[5].1HNMR spectraoftissuesufferfrommajorline broadeningcon tributionswhenacquiredbyconventionalsolution state NMRspectroscopybecau…     

Biochemical effects of gadolinium chloride in rats liver and kidney studied by ^1H NMR metabolomics

The biochemical effects of gadolinium chloride were studied using high-resolution IH nuclear magnetic resonance (NMR) spec-troscopy to investigate the biochemical composition of tissue (liver and kidney) aqueous extracts obtained from control and gadolinium chlo-ride (GdCl3) (10 and 50 mg/kg body weight, intraperitoneal injection, i.p.) treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCl3, and extracted using methanol/chloroform solvent system. 1H NMR spectra of tissue extracts were analyzed by pat-tern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and de-creased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine (GPC), betaine, myo-inositoi and trimethylamine N-oxide (TMAO)levels in kidney illustrated kidney disturbance induced by GdCl3.     

小波分析在处理代谢组学NMR信号中的应用

代谢组学是基于核磁共振(NMR)和模式识别的研究,信号的全面提取和分析是非常重要的.将小波分析用于1H NMR 信号的去噪平滑,先去除掉噪声信号的干扰,再通过主成分分析建立数学模型.结果表明,这是一种能够有效去除噪声信号的方法,可用于更为精确的定量分析,建立了较以往更为完善的代谢组学药物模型.     

Comparison of Biochemical Effects Induced by Changle between Male and Female Rats Using NMR and ICP-MS Techniques

NMRspectroscopyofbiofluidhasbroughtnew chemistryintolifescienceandclinicalmedicine,and NMRspectroscopy basedapproachhasbeensuccess fullyappliedtothestudiesofbiochemicaleffectsand inborndiseaseswiththeadventofhighfieldNMR spectrometers[1].Inrecentyears,highresolutionNMRhasbeenusedtotherapidmulticomponentanal ysisoflowmolecularweightcompoundsinbiofluid,includingurine,bile,cerebrospinalfluid,saliva,milkandsynovialfluidforclinicaldiagnosisofinborn diseasesandinvestigationofbiochemicaleffectsof…