正交法用于真菌微卫星PCR体系优化

目的: 探讨影响真菌微卫星聚合酶链反应（SSR-PCR）的多个因素，建立PCR的最佳反应体系，为进一步进行真菌的鉴定、遗传多样性研究奠定基础。方法: 利用正交设计，从Taq DNA聚合酶、模板DNA、dNTP、引物4 种因素，对真菌微卫星反应体系进行优化分析，并确定 PCR 适宜的退火温度及循环次数。结果: 各因素的不同水平对PCR结果都有显著 影响，其中模板DNA影响最大。在PCR反应体系中（25 μL），Taq　DNA 聚合酶 1U，模板DNA 30 ng ，dNTP 150 μmol•L^-1，引物 0.25 μmol•L^-1为最佳。 结论: 采用正交法 实验设计，对真菌微卫星PCR体系优化更科学，得出的实验体系有效范围宽，且高效、快捷。     

CEUS观察兔肝VX2肿瘤坏死演变

目的应用CEUS技术观察兔肝VX2肿瘤坏死过程,探讨VX2肿瘤生长坏死规律及其对实验产生的影响。方法应用超声引导下经皮穿刺种植法成功建立24只兔VX2肝肿瘤模型,并通过CEUS技术分别在肿瘤种植后10、14、21、28天动态观察肝肿瘤演变过程,测量肿瘤体积及坏死体积,计算瘤内坏死率。结果在肿瘤种植后10、14、21、28天兔肝VX2肿瘤体积、坏死灶体积和坏死率比较,差异均有统计学意义(P均0.05);肿瘤种植后10、14、21、28天的CEUS表现能反映出肿瘤的病理学特征。实验依据CEUS和病理特点将肿瘤生长过程细分为5个时期,0~10天为肿瘤实质前期;11~14天为肿瘤实质后期;15~21天为肿瘤坏死初期;22~28天为加速坏死期;28天后为肿瘤囊变期。结论 CEUS可准确判断肝肿瘤实质及坏死范围;VX2肿瘤的生长演变及肿瘤坏死具有规律性,可根据不同实验要求选择适宜的研究时间段。     

手术护理路径在经尿道等离子前列腺手术配合中的应用

目的 观察经尿道等离子前列腺手术中应用手术护理路径的效果。方法 将接受尿道等离子前列腺手术的45例患者随机分为2组,对照组20例患者接受常规护理,观察组25例患者接受手术护理路径护理,对2组患者的住院时间、护理不良事件发生情况以及健康教育知识掌握情况进行比较分析。结果 对照组患者住院时间为（10.13±1.64）d,观察组为（8.71±1.58）d;护理不良事件发生率对照组为15.0%,观察组为4.0%;健康知识评分对照组为23.48±1.47,观察组为27.86±1.45;2组患者3项指标比较,差异均有统计学意义（P〈0.05）。结论 经尿道等离子前列腺手术中应用手术护理路径较常规护理能缩短住院时间,降低不良事件发生率。     

24h动态血压非规律变化与左心室肥厚的相关性分析

长期以来高血压对心血管系统的影响主要依据血压的高低来估测心血管疾病的危险性，近年来亦有根据脉压的大小来预测心血管事件。本文通过对高血压患者进行24h动态血压监测和超声心动图检测，探讨血压非规律变化与左心室肥厚的相关性，以便早期发现及预防高血压左心室肥厚的发生。     

Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of comea blast injury was developed by using a single firecracker. For each rabbit, the right eye was the experimental eye (seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion) and the left eye was the control (treated with 0.9% sodium chloride). Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, yrd, 4th, 5th, and 7th days following seawater immersion or 0.9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190.338, 156. 107,117.018 ng/L respectively, and significant differences could be seen when comparisous were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.     

眼部结核2例

1病例报告例1:女,59岁,住院号304409,退休职员。患者发右上睑外侧包块1+mo,包块渐增大,无疼痛、复视、眼球动障碍,无发热、咳嗽、盗汗等。27a前因左眼新生血管青光眼,行左眼球摘除术。入院查体:右眼视力1.0,上外侧饱满,皮肤无明显充血,眼眶外上方触及条索状包块大小约1cm×0.5cm,后界不清,睑结膜面未触及,包块中,无明显压痛,眼球活动度正常,屈光介质透明,眼底见异常。左眼球缺如。颌下、耳前淋巴结未触及,心、肺腹部检查无明显阳性体征。眼眶CT报告:右侧泪腺区见不规则软组织密度影,与右侧眼球分界欠清晰,其内度尚均匀,CT值约32Hu,其大小约0.8cm×2cm,眶壁见骨质破坏。入院     

Effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits

Objective To observe effects of penetrating cornea injury combined with seawater immersion on the levels of aqueous humor TNF-α in rabbits. Methods 10 adult healthy grey rabbits were used for the experiment. The rabbit eye model of comea blast injury was developed by using a single firecracker. For each rabbit, the right eye was the experimental eye (seawater was injected into the anterior chamber through the cornea incision, simulating seawater immersion) and the left eye was the control (treated with 0.9% sodium chloride). Levels of aqueous humor TNF-α were detected by using double-antibody sandwich ELISA on the 1st, 2nd, yrd, 4th, 5th, and 7th days following seawater immersion or 0.9% sodium chloride injection. Results Following development of the model, levels of aqueous humor TNF-α in the experimental eye on the 1st, 2nd, 3rd days were 190.338, 156. 107,117.018 ng/L respectively, and significant differences could be seen when comparisous were made between the experimental and control eyes. The level of aqueous humor TNF-α on the 1st day was the highest and obviously reached the peak. Conclusions Level of aqueous humor TNF-α in the experimental eye with penetrating cornea injury combined with seawater immersion was significantly higher than that of the control eye, indicating that TNF-α was clearly involved in the pathological change and it played an important role in the process.     

脉络宁注射液治疗脑血管性痴呆64例

将６４例老年血管性痴呆患者,随机分为治疗组（脉络宁注射液组３６例）及对照组（维脑路通组２８例）,疗程均为２０ｄ。结果显示脉络宁在改善临床症状,提高长谷川痴呆量表分数及临床记忆商方面明显优于对照组（Ｐ＜０．０５）,且无毒副作用。说明脉络宁是一种理想的脑功能改善剂。     

糖尿病并发脑梗死患者的TCD分析

目的观察糖尿病并发脑梗死患者的经颅多普勒超声（TCD）改变。方法选取72例糖尿病并发脑梗死患者行TCD检查,分别经颞窗、枕窗常规检测两侧大脑中动脉（MCA）、大脑前动脉（ACA）、大脑后动脉（PCA）、椎动脉（VA）、基底动脉（BA）。结果72例患者中TCD正常5例（6.9%）,异常67例（93.1%）。主要为颅内大动脉血流速度加快、减慢、血管杂音、血管音频消失。结论糖尿病并发脑梗死患者TCD检查发现脑血流有显著改变,对脑梗死治疗及预防脑梗死复发提供了可靠的参考依据。     

RNA干扰XBP1基因表达对口腔鳞状细胞癌生物学特性的影响研究

目的:检测口腔鳞状细胞癌组织中X盒结合蛋白1(XBP1)的表达及观察抑制其表达后对人口腔鳞状细胞癌Tca8113增殖凋亡的影响,并探讨其机制。方法:RT-PCR检测42例口腔鳞状细胞癌及癌旁组织中XBP1基因的mRNA表达;NC-siRNA和XBP1-siRNA转染至Tca8113细胞内,不经任何处理的细胞作为空白对照组,48 h后,CCK8实验、流式细胞仪分别检测XBP1基因转染对细胞增殖、凋亡的影响;Western blot检测Cleaved caspase3、β-catenin、Cyclin D1蛋白表达的变化。结果:口腔鳞状细胞癌中XBP1基因的mRNA表达显著高于癌旁组织(P<0.01);转染XBP1-siRNA能够显著降低XBP1的蛋白表达,降低细胞的存活率,促进细胞的凋亡,上调Cleaved caspase3蛋白表达,下调β-catenin、Cyclin D1蛋白表达(P<0.01)。结论:RNA干扰沉默XBP1基因表达可通过抑制Wnt/β-catenin信号通路降低Tca8113增殖及诱导细胞凋亡。