Effects of natural pyrethrum and synthetic pyrethroids on the tiger mosquito,Aedes albopictus (skuse) and non-target flower-visiting insects in urban green areas of Padua,Italy

Abstract

The tiger mosquito is a key vector of several human diseases and is considered a public health concern worldwide. The implementation of strategies aimed at maximizing mosquito control without affecting non-target insect groups is of major importance. In a field trial, we tested the efficiency of a natural pyrethrum-based vs. a synthetic pyrethroid-based insecticide in reducing tiger mosquito population and how they affect the diversity of non-target flower-visiting insects in green urban areas. Only the pyrethroid insecticide was effective in reducing mosquito abundance, although its effects disappeared nine days after application. The two adulticides did not significantly affect the diversity of flower-visiting insects, probably because of their large body size and the difference in flying and foraging activity. To effectively control mosquito populations while preventing intoxication of non-target flower-visiting insects, adulticide applications should be applied early in the morning and only on bushes and trees. Results from our small-scale applications cannot be extrapolate when larger areas are treated.     

单克隆抗体ELISA检测犊牛粪便中冠状病毒抗原

用两株单克隆抗体混合后包被ＥＬＬＩＳＡ微孔板，加牛冠状病毒抗原，加经白陶土处理过的兔抗牛冠状病毒免血清，再加上辣根氧化物酶标记的羊抗兔ＩｇＧ抗体，加底物质显色间接ＥＬＩＳＡ检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液，其敏感度高出血凝试验（ＨＡ）８倍，从武汉市附近３个奶牛场采集犊牛腹泻粪便１０５份，用单抗ＥＬＩＳＡ检测牛冠状病毒抗原，共检出阳性３６份，并与血凝及血凝抑制试验，     

KK-42对家蚕蛹血淋巴及卵巢蛋白质合成的影响

研究了咪唑类化合物KK—42对家蚕卵巢发育以及蛋白质合成的影响,结果表明KK-42对卵巢增重有明显的抑制作用,血淋巴蛋白含量在蛹期变化幅度很小,卵巢蛋白质含量增加也很缓慢;而对照区在化蛹后,血淋巴蛋白急剧增加,第三天达到高峰,以后又迅速下降,与卵巢蛋白质增加相吻合。由此表明,咪唑类化合物KK-42不仅抑制血淋巴蛋质的合成,同时还抑制了血淋巴蛋白向卵巢的转移。     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

水貂铜绿假单胞菌检测方法研究进展

水貂出血性肺炎是由铜绿假单胞菌引起的威胁养貂业的重要疾病之一。为了解该病相关检测方法的最新研究进展,本文从病原学和分子生物学方面,介绍了细菌分离培养、生化试验、特异性抗原及其抗体检测、PCR技术和LAMP技术等。通过比较各种方法的优缺点和实用性,为临床兽医科技工作者快速、简便、准确、实用地诊断提供参考。     

猪附红细胞体生物学特性的研究

采取新鲜血液压片相衬观察和末梢血液涂片染色镜检法,对河南省新乡地区3000头猪附红细胞体病发病情况进行了调查;并且对体温升高、镜检附红细胞体感染强度为 病猪的血液,分别用光学显微镜、扫描电子显微镜进行形态观察和血液生化指标检测.结果表明:猪附红细胞体病的发病率为46.33%,死亡率为13.88%,隐性感染率为55.93%(755/1350).在光学显微镜下观察,红细胞呈现菠萝样;染色血片观察附红体在红细胞表面及边缘呈各种形式排列,可见强折光性发亮的圆形小体;扫描电子显微镜观察.附红体寄生的红细胞凹陷区,有大、小球体,有的小球体与大球体相连.血清胆红素、白蛋白、尿素氮、谷丙转氨酶、谷草转氨酶增加,血糖、血清总蛋白、超氧化物歧化酶活性下降.     

原料成分变异的管理——分析是关键

畜禽日粮成分的变异可以说是其固有性质。除了一些特定的饲料谷物如玉米之外,大部分日粮原料都是各类加工物的副产品。     

放养密度和投饲率对南美白对虾单产的影响

为了提高养殖南美白对虾的单产水平,采用梯度设计法和回归分析法研究了放养密度和日投饲率对养殖南美白对虾单产的影响.结果 表明,新疆北疆地区南美白对虾单产(Y)与放养密度(SD)呈极显著二次曲线关系Y=-0.0461SD2+ 11.204SD-78.959(R2=0.9707)(F＞Fn01),当地南美白对虾最佳放养密度为...     

施肥对甜菜产量、物质积累和养分吸收规律的影响

采用田间试验研究了施肥对甜菜产量、干物质积累和吸肥规律的影响。结果表明：氮、磷、钾肥配合施用可以提高甜菜产量,制约甜菜产量的养分因子依次为N〉P〉K。干物质日积累规律为：苗期增长缓慢,叶和块根分别在出苗后80,125d达到最大值,在60～80d时,块根的日积累量超过叶。氮的吸收速率,全株表现为慢-快-慢的规律,叶片和块根分别在出苗后100,160d达到最大值,氮的累积总量在叶中较多。磷的吸收速率,叶片表现为出苗后40～80d最快,块根在40～120d快速增加并达到最大值;磷在叶和根中的累积量分别在出苗后80,120d达到最大,80d之后根的累积量超过叶。对于钾,叶在出苗后40—120d快速吸收并达到最大值,块根在出苗60d后吸收加快,收获时达到最大;在整个生育期内块根对K的吸收量都低于叶。甜菜单株日吸收N和P量分别在出苗后100,60d达到最大值;K的日吸收量苗期较少,40～80d快速增加达到最大,收获前出现负增长。     

The peroxisomal matrix shuttling receptor Pex5 plays a role in FB1 production and virulence in Fusarium verticillioides

The peroxisomal matrix oxidase,catalase and peroxidase are imported peroxisomes through the shuttling receptors,which regulates the cellular oxidative homeostasis and function.Here,we report that PTS1 shuttling receptor FvPex5 is involved in the localization of PTS1,utilization of carbon sources and lipids,elimination ROS,cell wall stress,conidiation,fumonisin B₁(FB₁) production,and virulence in maize pathogen Fusarium verticillioides.Significantly,differential expression o...