抗犬细小病毒高免血清的制备和应用

应用差速离心和密度梯平衡离心技术从患ＣＰＶ性肠炎的犬粪便和肠内容物中提纯的ＣＰＶ抗原接种犬，制备了高效价的抗血清，用该血清共治疗７８头病犬，治愈率达８２％（６４／７８），实践表明，自制的高免血清对患ＣＰＶ性肠炎的病犬具有治疗和控制疾病发展的作用，特别是早期病例，仅用抗血清即可治愈，对中期病犬结合支持和对症疗法，可大大提高治愈率，有很高的应用价值。     

A Homoeotic Mutant of Arabidopsis thaliana with Leafy Cotyledons

Cotyledons are specialized leaves produced during plant embryogenesis. Cotyledons and leaves typically differ in morphology, ultrastructure, and patterns of gene expression. The leafy cotyledon (Iec) mutant of Arabidopsis thaliana fails to maintain this distinction between embryonic and vegetative patterns of plant development. Mutant embryos are phenotypically abnormal, occasionally viviparous, and intolerant of desiccation. Mutant cotyledons produce trichomes characteristic of leaves, lack embryo-specific protein bodies, and exhibit a vascular pattern intermediate between that of leaves and cotyledons. These results suggest that lec cotyledons are partially transformed into leaves and that the wild-type gene (LEC) functions to activate a wide range of embryo-specific pathways in higher plants.     

抗绵羊进行性肺炎病毒单克隆抗体杂交瘤细胞株的建立

用提纯的OPPV细胞抗原免疫BALB/c小鼠脾脏与SP2/0小鼠骨髓细胞融合,融合率为70%,以ELISA双抗体夹心法进行检测,阳性率为20%,经过有限稀释法克隆出1A₆、2B₈、1G₃、2G₄4株分泌抗OPPV抗原的单克隆体杂交瘤细胞株.选择抗体分泌滴度高的1A₆细胞进行了较详细的研究,结果表明,其核内染色体数为96～110条,抗体属性为IgG1,轻链为K链,IFA试验表明产生的McAb对OPPV有特异性,细胞传30代并在液氮中保存半年后复苏仍能稳定地分泌抗OPPV McAb,细胞分泌抗体的ELISA滴度腹水为6.4×10⁴,上清为640,McAb经硫基乙醇还原后在SDS-PAG上呈现2条区带,一条为重链部分,MW50000,另一条为轻链部分,MW为25000.     

小谷粒作物赤霉病评述（Ⅰ）

本文评述了小类作赤霉病，与该病有关的病原物有１７种，这些病原物发生在世界在部分谷类作物种植最常见的禾谷镰刀菌、黄色镰刀菌、燕麦镰刀菌梨孢镰刀菌、和雪腐镰刀菌。该病发生在湿气气候条件下，在此条件下谷物产量损失和毒物聚集显著。作物残体、替换寄主、以及禾谷类作物的镰刀菌菌腐和根腐是该病的可能接种体来源。     

5-HT4(a) receptors avert opioid-induced breathing depression without loss of analgesia

Opiates are widely used analgesics in anesthesiology, but they have serious adverse effects such as depression of breathing. This is caused by direct inhibition of rhythm-generating respiratory neurons in the Pre-Boetzinger complex (PBC) of the brainstem. We report that serotonin 4(a) [5-HT4(a)] receptors are strongly expressed in respiratory PBC neurons and that their selective activation protects spontaneous respiratory activity. Treatment of rats with a 5-HT4 receptor-specific agonist overcame fentanyl-induced respiratory depression and reestablished stable respiratory rhythm without loss of fentanyl's analgesic effect. These findings imply the prospect of a fine-tuned recovery from opioid-induced respiratory depression, through adjustment of intracellular adenosine 3',5'-monophosphate levels through the convergent signaling pathways in neurons.     

Spectroscopic characterization of mineralogy and its diversity across Vesta

The mineralogy of Vesta, based on data obtained by the Dawn spacecraft's visible and infrared spectrometer, is consistent with howardite-eucrite-diogenite meteorites. There are considerable regional and local variations across the asteroid: Spectrally distinct regions include the south-polar Rheasilvia basin, which displays a higher diogenitic component, and equatorial regions, which show a higher eucritic component. The lithologic distribution indicates a deeper diogenitic crust, exposed after excavation by the impact that formed Rheasilvia, and an upper eucritic crust. Evidence for mineralogical stratigraphic layering is observed on crater walls and in ejecta. This is broadly consistent with magma-ocean models, but spectral variability highlights local variations, which suggests that the crust can be a complex assemblage of eucritic basalts and pyroxene cumulates. Overall, Vesta mineralogy indicates a complex magmatic evolution that led to a differentiated crust and mantle.