全文获取类型
收费全文 | 320篇 |
免费 | 12篇 |
国内免费 | 34篇 |
学科分类
医药卫生 | 366篇 |
出版年
2024年 | 2篇 |
2023年 | 7篇 |
2022年 | 7篇 |
2021年 | 19篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 5篇 |
2016年 | 6篇 |
2015年 | 4篇 |
2014年 | 12篇 |
2013年 | 18篇 |
2012年 | 6篇 |
2011年 | 10篇 |
2010年 | 11篇 |
2009年 | 18篇 |
2008年 | 17篇 |
2007年 | 27篇 |
2006年 | 17篇 |
2005年 | 18篇 |
2004年 | 5篇 |
2003年 | 6篇 |
2002年 | 2篇 |
2001年 | 11篇 |
2000年 | 6篇 |
1998年 | 9篇 |
1997年 | 17篇 |
1996年 | 10篇 |
1995年 | 11篇 |
1994年 | 8篇 |
1993年 | 6篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 7篇 |
1989年 | 3篇 |
1988年 | 10篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 6篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 1篇 |
排序方式: 共有366条查询结果,搜索用时 0 毫秒
1.
Sukal Sean A. MD PhD † Tudisco Marie HT † Strippoli Barbara HT † Nehal Kishwer S. MD † 《Dermatologic surgery》2005,31(7):763-766
Background Processing multiple tissue sections in large Mohs cases is time consuming and labor intensive.
Objective To present innovative laboratory techniques to facilitate processing of large Mohs cases.
Methods A method for processing a large dermatofibrosarcoma protuberans Mohs case is outlined.
Results Modifications in tissue processing and equipment employed in a large Mohs case are presented.
Conclusion Innovative modifications to the standard Mohs laboratory technique can facilitate processing of large Mohs cases, resulting in high-quality, rapid frozen sections while optimizing efficiency. 相似文献
Objective To present innovative laboratory techniques to facilitate processing of large Mohs cases.
Methods A method for processing a large dermatofibrosarcoma protuberans Mohs case is outlined.
Results Modifications in tissue processing and equipment employed in a large Mohs case are presented.
Conclusion Innovative modifications to the standard Mohs laboratory technique can facilitate processing of large Mohs cases, resulting in high-quality, rapid frozen sections while optimizing efficiency. 相似文献
2.
3.
BACKGROUND: The phenomenon of wound contraction results in a decrease in wound size and a healed scar significantly smaller than the original defect. OBJECTIVE: This study was undertaken (1) to determine the amount of wound contraction in Mohs surgery defects allowed to heal by second intention, (2) to evaluate for regional differences in wound contraction based on the facial anatomic zones for second intention healing described by Zitelli, and (3) to determine whether regional differences in wound contraction account for observed differences in cosmetic outcome. METHODS: One hundred sixty secondarily healed Mohs surgery defects limited to the head and neck having a wound age of greater than 12 weeks in 102 consecutively examined patients were carefully measured with a tissue caliper. The percent wound contraction was calculated and compared for each Zitelli anatomic subunit. The final shape of the wound (quantitatively described) and the cosmetic acceptability (subjectively rated by the patient and examiner) were also compared with the percent wound contraction for each anatomic area. RESULTS: Both NEET (concave surface of the nose, eye, ear, and temple) and FAIR (forehead, antihelix, eyelids, and the remainder of the nose, lips, and cheeks) areas were identical in terms of mean wound contraction (74%), cosmetic acceptability (97%), and conversion to a wound shape with a ratio of maximal length to width of greater than 3.0 (fusiform and linear shapes) (52%). NOCH areas (convex surface of the nose, oral lips, cheeks and chin, and the helix of the ear) demonstrated less wound contraction (66%), cosmetic acceptability (78%), and fusiform-linear conversion (29%). Subset differences and variables that appear to influence wound contraction are discussed. Secondarily healed wounds in areas with one or more positive contraction variables contract 75%, whereas defects in areas with negative contraction variables contract 55%. CONCLUSIONS: Regional differences in wound contraction of secondarily healed head and neck wounds exist and account for some differences in cosmetic acceptability. Scar location, regardless of the degree of wound contraction, is the most important factor for the final cosmetic outcome. 相似文献
4.
5.
Cloning and developmental expression analysis of the murine homolog of the spinocerebellar ataxia type 1 gene (Sca1) 总被引:2,自引:0,他引:2
Banfi S; Servadio A; Chung M; Capozzoli F; Duvick LA; Elde R; Zoghbi HY; Orr HT 《Human molecular genetics》1996,5(1):33-40
Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant
neurodegenerative disorder caused by the expansion of a CAG trinucleotide
repeat which encodes glutamine in the novel protein ataxin-1. In order to
characterize the developmental expression pattern of SCA1 and to identify
putative functional domains in ataxin-1, the murine homolog (Sca1) was
isolated. Cloning and characterization of the murine Sca1 gene revealed
that the gene organization is similar to that of the human gene. The murine
and human ataxin-1 are highly homologous but the CAG repeat is virtually
absent in the mouse sequence suggesting that the polyglutamine stretch is
not essential for the normal function of ataxin-1 in mice. Cellular and
developmental expression of the murine homolog was examined using RNA in
situ hybridization. During cerebellar development, there is a transient
burst of Sca1 expression at postnatal day 14 when the murine cerebellar
cortex becomes physiologically functional. There is also marked expression
of Sca1 in mesenchymal cells of the intervertebral discs during development
of the spinal column. These results suggest that the normal Sca1 gene, has
a role at specific stages of both cerebellar and vertebral column
development.
相似文献
6.
Kirstin Vach Ali Al-Ahmad Annette Anderson Johan Peter Woelber Lamprini Karygianni Annette Wittmer Elmar Hellwig 《Nutrients》2021,13(3)
Background: Investigating the influence of nutrition on oral health has a long scientific history. Due to recent technical advances like sequencing techniques for the oral microbiota, this topic has gained scientific interest again. A basic challenge is to understand the influence of nutrition on the oral microbiota and on the interaction between the oral bacteria, which is also statistically challenging. Methods: Log-transformed ratios of two bacteria concentrations are introduced as the basic analytic tool. The framework is illustrated by application in an experimental study exposing eleven participants to different nutrition schemes in five consecutive phases. Results: The method could be sufficiently used to analyse the interrelation between the bacteria and to identify some bacterial groups with the same as well as different reactions to additional dietary components. It was found that the strongest changes in bacterial concentrations were achieved by the additional consumption of dairy products. Conclusion: A log ratio-based analysis offers insights into the relation of different bacteria while taking specific features of compositional data into account. The presented methods allow becoming independent of the behaviour of other bacteria, which is a disadvantage of common analysis methods of compositions. The results indicate that modulations of the oral biofilm microbiota due to nutrition change can be attained. 相似文献
7.
Alexandra Schneider-Chaabane David Boschert Sibylle Rau Diana Lorena Guevara Solarte Vera Bleicher Ali Al-Ahmad Karen Lienkamp 《Macromolecular chemistry and physics.》2023,224(5):2200323
Facially amphiphilic polymers carrying cationic and hydrophobic groups on the same repeat unit have shown promising antimicrobial activity and biocompatibility, yet they are prone to suffer from protein adhesion which may induce biofilm formation. To overcome this problem, poly(diitaconate)-based copolymers with cationic/hydrophobic and protein-repellent/charge-neutral repeat units are synthesized. The bioactivity profile of surface-attached polymer networks made from these copolymers depends on the ratio of the cationic and charge-neutral repeat units. In all cases, the protein adhesion is substantially reduced compared to purely cationic polymers. At a 50:50 ratio, the polymer coatings are partially protein-repellent and antimicrobial, yet slightly cell toxic. At an intermediate composition of 30:70, they are still antimicrobial and the cell compatibility is substantially improved. The long-term stability of these materials still has to be determined to judge their suitability for medical applications. 相似文献
8.
C Dezateux HT Delves J Stocks A Wade L Pilgrim K Costeloe 《Archives of disease in childhood》1997,76(5):432-436
OBJECTIVE: To determine whether antimony may be detected in the urine during infancy and early childhood and its association with passive exposure to tobacco smoke, as assessed by urinary cotinine. DESIGN: Analysis of spare aliquots of urine collected from infants participating in studies of respiratory function and passive smoking. Urinary antimony was assayed using inductively coupled plasma mass spectroscopy in 201 urine specimens collected at different ages throughout the first two years of life from 122 term and 26 preterm infants. Urinary cotinine was measured using gas liquid chromatography. MAIN OUTCOME MEASURE: Urinary antimony concentrations. RESULTS: Absolute antimony concentrations varied widely between infants, being below the laboratory detection limit of 0.02 microgram/l in 7% of samples, below 0.5 microgram/l in 90.5%, and above the reference value of 1 microgram/l reported for non-occupationally exposed UK populations in 4%. Creatinine standardised antimony values were unrelated to postnatal age or urinary cotinine concentrations and were highest in urine collected from preterm infants within 24 hours of birth (geometric mean (95% confidence interval): 2.3 ng/mg (1.5 to 3.4)). CONCLUSIONS: Although antimony is present at very low concentrations in urine during infancy and early childhood, the relevance to health is uncertain. The higher levels found in preterm infants may reflect prematurity or fetal assimilation of antimony. Tobacco is unlikely to be an important source of environmental exposure to antimony during infancy and early childhood. 相似文献
9.
10.
Domenico G. Della Rocca MD Michele Magnocavallo MD Veronica N. Natale MPH Carola Gianni MD PhD Sanghamitra Mohanty MD Chintan Trivedi MD MPH Carlo Lavalle MD Giovanni B. Forleo MD PhD Nicola Tarantino MD Jorge Romero MD Xiadong Zhang MD Mohamed Bassiouny MD Amin Al-Ahmad MD David J. Burkhardt MD Joseph G. Gallinghouse MD Javier E. Sanchez MD Rodney P. Horton MD Luigi Di Biase MD PhD Andrea Natale MD 《Journal of cardiovascular electrophysiology》2021,32(9):2441-2450