毛细管电泳差异性检测尿液蛋白方法初探

Objective: To explore an optimized method for difference detection of urinary proteomics in patients with Bladder Cancer (BCs) by Capillary Electrophoresis separation technique coupled with diode array detector (CE-DAD). Methods: For detecting the difference in the contents of each urinary low-abundance proteins between the two groups, electrolytic additives were used to improve the buffer system, and the concentration of electro-osmotic flow was well controlled in this condition, in order to increase the separation efficiency. Results: The optimal conditions were established to detect the difference between the bladder cancer and the control group by CE-DAD, and the difference and correlation were also tested by the parallel experiment of ELISA method. Conclusion: This paper makes a preliminary exploration on the condition of difference detection by CE-DAD, and establishes the foundation for the institution of standardized experimental method in the future.