微球动态涂敷状态实时监测光学系统的设计

 介绍了一种可用于实时监测直径0.2～1.0 mm激光核聚变靶球涂敷状态的光学系统的设计，系统采用环形LED照明系统以适合特殊的照明要求，用Petzval型物镜使500 mm处的微球清晰成像于CCD像面上，CCD输出的图像电信号经图像采集卡转换成数字信号，最后该数字信号由计算机进行处理，实现了系统对靶球膜层涂敷作业的自动监控，大大提高了涂敷效率。所设计的系统轴上点最大弥散斑直径为12.6 mm,轴外最大弥散斑直径为15.8 mm，整个视场的像质比较均匀,分辨率较高，对于波长522 nm的光线，场曲和畸变分别小于15 mm和0.012%，像质优良。     

Investigation of Bessel beam propagation in scattering media with scalar diffraction method

Bessel beam propagation in scattering media is simulated using the angular spectrum method combined with slice-by-slice propagation model. Generating Bessel beams with a spatial light modulator, which provides a means to adjust flexibly the parameters of the Bessel beam, allows us to validate the simulation results experimentally. The study reveals that the self-reconstructing length changes oppositely with the axicon angle （i.e., the larger the axieon angle, the shorter the self-reconstructing length）. The radius of the incident beam has little influence on the self-reconstruction of the Bessel beam central lobe.     

Dual-channel phase-shifting interferometry for microscopy with second wavelength assistance

Dual-channel phase-shifting interferometry for simultaneous phase microscopy is presented. Red and blue light beams are used for microscope illumination. A 45 tilted beamsplitter replicates the object and reference waves in red light together with the object wave in blue light into two parallel beams. The two resulting quadrature phase-shifting interferograms in red light and the object waves in blue light are generated in the two channels. The two interferograms are recorded simultaneously by a color charge-coupled device (CCD) camera, and can be separated via RGB components of the recorded color patterns without crosstalk. As a result, the phase of tested specimen can be retrieved. The feasibility of the proposed method is demonstrated by test performed on a microscopic specimen.     

Reflective point-diffraction microscopic interferometer with long-term stability

An on-axis phase-shifting reflective point-diffraction microscopic interferometer for quantitative phase microscopy based on Michelson architecture is proposed.A cube beamsplitter splits the object wave spectrum into two copies within two arms.Reference wave is rebuilt in one arm by low-pass filtering on the object wave frequency spectrum with a pinhole-mask mirror,and interferes with the object wave from the other arm.Polarization phase-shifting is performed and phase imaging on microscale specimens is implemented.The experimental results demonstrate that the proposed scheme has the advantage of long-term stability due to its quasi common-path geometry with full use of laser energy.     

高精度定量相位显微成像方法研究

定量相位显微成像在工业检测、生物医学和光场调控等领域具有重要的应用价值。常用的定量相位显微成像技术通过干涉的方法来获取相位的定量分布,干涉装置的稳定性、光学衍射极限的限制、相位再现时的解包裹问题、激光照明下的相干噪声,以及动态观测过程中的样品离焦等因素都会影响定量相位显微成像的分辨率和精度。本文围绕高精度定量相位显微成像中的上述关键问题展开研究,通过构建物参共路的同步相移数字全息显微结构实现稳定的实时测量;采用结构光照明的超分辨相位成像方法实现对微小物体的超分辨相位成像;利用双波长照明将纵向无包裹相位测量范围扩大到微米量级;使用低相干LED照明解决相干噪声问题;提出了基于结构光照明和双波长照明的数字全息显微自动调焦方法,可以满足对不同类型样品的长时间跟踪观测。     

基于光强传输方程相位成像的宽场相干反斯托克斯拉曼散射显微背景抑制

相干反斯托克斯拉曼散射(CARS)显微能够对样品的特殊化学组分进行选择性成像,无需荧光标记,在生物医学领域被广泛应用.然而,传统的CARS图像往往存在非共振背景信号.本文将基于光强传输方程的单光束相位成像技术用于CARS显微成像,来抑制CARS的非共振背景信号.该方法通过记录样品在三个相邻平面上的CARS图像,然后利用光强传输方程获取CARS光场的相位分布,最后利用共振CARS信号和非共振背景信号在相位上的差异,实现了对背景噪声的抑制.该方法无需参考光,通过三次测量可完成CARS的背景噪声抑制,具有良好的应用前景.