纳米锶磷灰石的体外细胞毒性研究

目的对纳米锶磷灰石进行体外细胞毒性试验,评价其生物安全性,为将其应用于骨折临床修复奠定基础。方法将不同浓度的纳米锶磷灰石浸提液与小鼠成纤维细胞L929体外共培养2d、4d、7d,倒置相差显微镜下观察细胞的形态变化;用四氮唑盐比色法(MTT)检测,计算细胞相对增殖度,用六级毒性分类法进行评级。结果培养期细胞贴壁生长,形态良好,随着培养天数的增加细胞大量增殖,毒级为0～1级。相同时间点不同浓度的纳米锶磷灰石浸提液的吸光度值无明显差异性(p>0.05)。结论初步证实纳米锶磷灰石具有人体应用的生物安全基础,无细胞毒性。     

纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究

Objective To evaluate the effect of titanium fiber mesh microballoons combined with nano-Sr-HAP on repair of bone defects in vivo. Methods The experimental model was set up by creating drill defects in the bilateral femoral condyle of 24 SD rats [6 months, with the mean weight of (545±22 g)]. The left femoral defects were filled with titanium fiber mesh microballoons combined with nano-Sr-HAP, and the right with pure metal scaffolds. At the 1st, 2nd, 4th, and 8th week after surgery, the specimens were killed and taken the X-ray, histology, and histomorphology observation. The results were contrasted and ana-lyzed. Results X-ray observation showed that the bone defects in both sides were repaired. Histological re-sults showed that the titanium fiber mesh microballoons allowed the new bones growing inside, and more new bones in the left implants compared with those in the right sides. The results of histomorphometrical analysis confirmed that neo-bones in the left sides gradually increased as time passed. There were statistic differences in neo-bones at the 2nd, 4th, and 8th week, and the differences between the two sides were statistically sig-nificant at the 4th and 8th week. Conclusion As titanium fiber mesh microballoons used in this experiment have good biocompatibility and osteoconduction ability, they can be used as scaffold for bone defect repair; and Nano-Sr-HAP can enhance the repair ability of titanium fiber mesh.     

不同分散方法对纳米ZrO2/PMMA复合材料挠曲强度的影响

目的:研究不同分散方法对氧化锆(zrO2)纳米填料/聚甲基丙烯酸甲酯(PMMA)复合材料试件挠曲强度的影响.方法:在丙酮溶液中用硅烷偶联剂Z-6030对纳米ZrO2颗粒进行表面修饰,将经过表面修饰后的纳米ZrO2颗粒按照3%的添加量加入到义齿基托树脂Ⅱ型粉剂中,然后分别采用手工、球磨和超声湿混3种方法予以分散混合,并以不添加纳米ZrO2颗粒的PMMA作为空白对照组,再与Ⅱ型液剂反应后制成4组标准试件,最后进行三点弯曲测试.实验数据采用SAS6.12软件包进行单因素方差分析,均数间进行两两比较(Newman-Keuls检验).结果:复合材料的挠曲强度以球磨组最高,达73.64 MPa,手工混合组最低,为62.86 MPa.球磨、超声2组的挠曲强度显著高于手工组和空白对照组(P<0.05).球磨和超声2组之间无显著差异(P>0.05).结论:以球磨、超声2种方法混合纳米ZrO2颗粒和PMMAⅡ型粉剂,可得到较好的分散效果,从而提高复合树脂的挠曲强度.     

纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究

Objective To evaluate the effect of titanium fiber mesh microballoons combined with nano-Sr-HAP on repair of bone defects in vivo. Methods The experimental model was set up by creating drill defects in the bilateral femoral condyle of 24 SD rats [6 months, with the mean weight of (545±22 g)]. The left femoral defects were filled with titanium fiber mesh microballoons combined with nano-Sr-HAP, and the right with pure metal scaffolds. At the 1st, 2nd, 4th, and 8th week after surgery, the specimens were killed and taken the X-ray, histology, and histomorphology observation. The results were contrasted and ana-lyzed. Results X-ray observation showed that the bone defects in both sides were repaired. Histological re-sults showed that the titanium fiber mesh microballoons allowed the new bones growing inside, and more new bones in the left implants compared with those in the right sides. The results of histomorphometrical analysis confirmed that neo-bones in the left sides gradually increased as time passed. There were statistic differences in neo-bones at the 2nd, 4th, and 8th week, and the differences between the two sides were statistically sig-nificant at the 4th and 8th week. Conclusion As titanium fiber mesh microballoons used in this experiment have good biocompatibility and osteoconduction ability, they can be used as scaffold for bone defect repair; and Nano-Sr-HAP can enhance the repair ability of titanium fiber mesh.     

用于牙菌斑pH测定的复合型微pH电极的研制

目的　研制可用于测定菌斑pH值的微pH电极。方法　用循环伏安法聚苯胺修饰钨丝电极作为pH指示电极 ,用Ag AgCl丝作为参比电极 ,二者复合于塑料套管内构成复合型pH微电极 ,试用于菌斑pH的测定。结果　在pH3～ 12范围内 ,该电极呈现Nernst响应 ,响应时间 <2min。将该电极试用于菌斑pH测定 ,获得蔗糖含漱后菌斑pH变动曲线。结论　该电极可用于菌斑内pH测定 ,且有望用于体内其它微小环境pH测定。     

纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究

目的 探讨纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的能力及其作用机制.方法 6月龄雄性SD大鼠24只,体重(545±22)g.在双侧股骨髁部使用慢速钻钻取直径2 mm贯通双侧皮质的冠状轴洞性骨缺损.以纤维多孔钛微球复合纳米锶磷灰石填充左侧骨缺损,以单纯纤维多孔钛微球填充右侧.术后1、2、4、8周分别处死6只大鼠行X线、组织学及骨组织形态计量学观察,并进行比较分析.结果 影像学结果表明,两侧骨缺损修复效果均良好.组织学显示,纤维多孔钛微球允许骨长入,且左侧微球内新生骨多于右侧.骨组织形态计量学观察显示,随时间延长,左侧新生骨量逐渐增多,2、4、8周新生骨量存在差异,而4、8周时左侧与右侧的新生骨量比较,差异有统计学意义.结论 纤维多孔钛微球具有良好的生物相容性及骨传导性,可作为骨缺损修复的支架材料;纳米锶磷灰石可增强纤维多孔钛微球修复骨缺损的能力.     

纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究

Objective To evaluate the effect of titanium fiber mesh microballoons combined with nano-Sr-HAP on repair of bone defects in vivo. Methods The experimental model was set up by creating drill defects in the bilateral femoral condyle of 24 SD rats [6 months, with the mean weight of (545±22 g)]. The left femoral defects were filled with titanium fiber mesh microballoons combined with nano-Sr-HAP, and the right with pure metal scaffolds. At the 1st, 2nd, 4th, and 8th week after surgery, the specimens were killed and taken the X-ray, histology, and histomorphology observation. The results were contrasted and ana-lyzed. Results X-ray observation showed that the bone defects in both sides were repaired. Histological re-sults showed that the titanium fiber mesh microballoons allowed the new bones growing inside, and more new bones in the left implants compared with those in the right sides. The results of histomorphometrical analysis confirmed that neo-bones in the left sides gradually increased as time passed. There were statistic differences in neo-bones at the 2nd, 4th, and 8th week, and the differences between the two sides were statistically sig-nificant at the 4th and 8th week. Conclusion As titanium fiber mesh microballoons used in this experiment have good biocompatibility and osteoconduction ability, they can be used as scaffold for bone defect repair; and Nano-Sr-HAP can enhance the repair ability of titanium fiber mesh.     

纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究

Objective To evaluate the effect of titanium fiber mesh microballoons combined with nano-Sr-HAP on repair of bone defects in vivo. Methods The experimental model was set up by creating drill defects in the bilateral femoral condyle of 24 SD rats [6 months, with the mean weight of (545±22 g)]. The left femoral defects were filled with titanium fiber mesh microballoons combined with nano-Sr-HAP, and the right with pure metal scaffolds. At the 1st, 2nd, 4th, and 8th week after surgery, the specimens were killed and taken the X-ray, histology, and histomorphology observation. The results were contrasted and ana-lyzed. Results X-ray observation showed that the bone defects in both sides were repaired. Histological re-sults showed that the titanium fiber mesh microballoons allowed the new bones growing inside, and more new bones in the left implants compared with those in the right sides. The results of histomorphometrical analysis confirmed that neo-bones in the left sides gradually increased as time passed. There were statistic differences in neo-bones at the 2nd, 4th, and 8th week, and the differences between the two sides were statistically sig-nificant at the 4th and 8th week. Conclusion As titanium fiber mesh microballoons used in this experiment have good biocompatibility and osteoconduction ability, they can be used as scaffold for bone defect repair; and Nano-Sr-HAP can enhance the repair ability of titanium fiber mesh.     

硅烷偶联剂的用量对PMMA/纳米ZrO2复合材料挠曲性能的影响

目的：研究硅烷偶联剂Z-6030的不同用量对聚甲基丙烯酸甲酯（PMMA）／纳米ZrO2复合材料挠曲强度的影响。方法：采用不同用量的硅烷偶联剂Z-6030，在丙酮溶液中对纳米ZrO2颗粒进行表面修饰，将经过表面修饰的纳米ZrO2颗粒按照3．0％的添加量，利用原位聚合生成法，与义齿基托树脂（聚甲基丙烯酸甲酯，PMMA）11型粉剂及液剂聚合生成分散良好的PMMA／纳米ZrO2义齿基托复合材料，参照IS01567：1999的标准，制作实验组的标准试件。用未添加纳米ZrO2颗粒及偶联剂的义齿基托树脂（聚甲基丙烯酸甲酯，PMMA）Ⅱ型粉剂及液剂制作普通基托组的标准试件。用添加未经表面修饰的纳米ZrO2颗粒的义齿基托树脂（聚甲基丙烯酸甲酯，PMMA）Ⅱ型粉剂及液剂制作对照组的标准试件。用三点弯曲试验测试材料的挠曲强度，采用SAS6．12软件包对结果进行单因素方差分析。结果：硅烷偶联剂Z-6030的用量为3．5％组的挠曲强度最大，与普通基托组、对照组、2．0％组、4．0％组、4．5％组、5．0％组有显著性差异（P〈0．05），0．5％组、1．0％组、1．5％组、2．5％组、3．0％组、3．5％组各组间的挠曲强度差异无统计学意义（P〉0．05）。0．5％组、1．0％组、1．5％组、2．0％组、2．5％组、3．0％组、4．0％组、4．5％组、5．0％组、普通基托组及对照组各组之间的挠曲强度的差异无统计学意义（P〉0．05）。结论：适当用量的硅烷偶联剂Z-6030可以提高PMMA／纳米ZrO2义齿基托复合材料的挠曲强度，硅烷偶联剂Z-6030的最佳用量为纳米ZrO2颗粒质量的3．5％。     

纤维多孔钛微球复合纳米锶磷灰石修复骨缺损的实验研究

Objective To evaluate the effect of titanium fiber mesh microballoons combined with nano-Sr-HAP on repair of bone defects in vivo. Methods The experimental model was set up by creating drill defects in the bilateral femoral condyle of 24 SD rats [6 months, with the mean weight of (545±22 g)]. The left femoral defects were filled with titanium fiber mesh microballoons combined with nano-Sr-HAP, and the right with pure metal scaffolds. At the 1st, 2nd, 4th, and 8th week after surgery, the specimens were killed and taken the X-ray, histology, and histomorphology observation. The results were contrasted and ana-lyzed. Results X-ray observation showed that the bone defects in both sides were repaired. Histological re-sults showed that the titanium fiber mesh microballoons allowed the new bones growing inside, and more new bones in the left implants compared with those in the right sides. The results of histomorphometrical analysis confirmed that neo-bones in the left sides gradually increased as time passed. There were statistic differences in neo-bones at the 2nd, 4th, and 8th week, and the differences between the two sides were statistically sig-nificant at the 4th and 8th week. Conclusion As titanium fiber mesh microballoons used in this experiment have good biocompatibility and osteoconduction ability, they can be used as scaffold for bone defect repair; and Nano-Sr-HAP can enhance the repair ability of titanium fiber mesh.