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排序方式: 共有274条查询结果,搜索用时 15 毫秒
1.
G P Gerrits A A Haagen R A De Abreu L A Monnens F J Gabre?ls F J Trijbels A L Theeuwes J M van Baal 《Clinical chemistry》1988,34(7):1439-1442
Disturbances in the metabolism of purines and pyrimidines in neurologically affected patients can be reflected by aberrant concentrations of nucleosides and nucleobases in cerebrospinal fluid (CSF). However, normal values, especially for children at different ages, are lacking. We collected 1000 specimens of CSF from subjects ranging in age from newborn to 18 years, who were undergoing a diagnostic lumbar puncture for several clinical indications. Of these, 78 samples could be used retrospectively as a reference according to our criteria. The analyses were performed with a modified HPLC procedure. None of the substances shows age-dependency except uridine and uric acid. Uridine increases with age, and uric acid increases with age in boys older than 12 years. 相似文献
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Haberham ZL van den Brom WE Venker-van Haagen AJ de Groot HN Baumans V Hellebrekers LJ 《Brain research》2000,873(2):287-290
We investigated whether components from the rat Vx-MLAEP could be used to assess depth of anaesthesia induced by propofol. Propofol decreased MLAEP amplitudes and increased latencies. We propose that the P(16)-N(22) wave in the rat MLAEP is similar to the human P1, and that recovery of this wave during propofol anaesthesia correlates with behavioural measures of the regaining of consciousness. 相似文献
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PU.1 as an essential activator for the expression of gp91phox gene in human peripheral neutrophils, monocytes, and B lymphocytes 总被引:6,自引:0,他引:6 下载免费PDF全文
Shoichi Suzuki Atsushi Kumatori Inez-Anne Haagen Yoshito Fujii Mohamed Anowar Sadat Hao Li Jun Yoshiro Tsuji Dirk Roos Michio Nakamura 《Proceedings of the National Academy of Sciences of the United States of America》1998,95(11):6085-6090
We have reported a deficiency of a 91-kDa glycoprotein component of the phagocyte NADPH oxidase (gp91phox) in neutrophils, monocytes, and B lymphocytes of a patient with X chromosome-linked chronic granulomatous disease. Sequence analysis of his gp91phox gene revealed a single-base mutation (C → T) at position −53. Electrophoresis mobility-shift assays showed that both PU.1 and hematopoietic-associated factor 1 (HAF-1) bound to the inverted PU.1 consensus sequence centered at position −53 of the gp91phox promoter, and the mutation at position −53 strongly inhibited the binding of both factors. It was also indicated that a mutation at position −50 strongly inhibited PU.1 binding but hardly inhibited HAF-1 binding, and a mutation at position −56 had an opposite binding specificity for these factors. In transient expression assay using HEL cells, which express PU.1 and HAF-1, the mutations at positions −53 and −50 significantly reduced the gp91phox promoter activity; however, the mutation at position −56 did not affect the promoter activity. In transient cotransfection study, PU.1 dramatically activated the gp91phox promoter in Jurkat T cells, which originally contained HAF-1 but not PU.1. In addition, the single-base mutation (C → T) at position −52 that was identified in a patient with chronic granulomatous disease inhibited the binding of PU.1 to the promoter. We therefore conclude that PU.1 is an essential activator for the expression of gp91phox gene in human neutrophils, monocytes, and B lymphocytes. 相似文献
5.
A diminished tolerance to the normal gut bacterial flora has been suggested to be pathogenic in ulcerative colitis (UC) and the aim of this study was to evaluate the priming effect of selected bacterial wall products on UC neutrophil granulocytes. Neutrophils from 10 UC patients and 10 healthy controls were primed with bacterial lipoprotein (BLP) or lipopolysaccharide (LPS) and subsequently activated. Extracellular superoxide production was measured by the cytochrome c reduction assay. Priming neutrophils with BLP or LPS dose dependently increased the superoxide production in both UC and controls (P < 0.01), and BLP was more potent than LPS (P < 0.05). No differences were found between UC and controls. UC neutrophils do not seem to have an intrinsic abnormality with reduced tolerance to bacterial substances. However, bacterial wall products such as BLP modify neutrophil tissue-destruction mechanisms and might be pivotal for perpetuation of chronic colonic inflammation. 相似文献
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Nelen WL Hermens RP Mourad SM Haagen EC Grol RP Kremer JA 《Human reproduction (Oxford, England)》2007,22(4):916-918
Monitoring reproductive health by the Reprostat indicators in Europe will facilitate the transparency of reproductive health as well as comparisons over time and between countries. However, for the monitoring and improvement of reproductive health care, we suggest the systematic development of evidence-based quality indicators, especially process and structure indicators. 相似文献
9.
Contribution of creatine kinase MB mass concentration at admission to early diagnosis of acute myocardial infarction. 总被引:1,自引:0,他引:1 下载免费PDF全文
A J Bakker J P Gorgels B van Vlies M J Koelemay R Smits J G Tijssen F D Haagen 《Heart (British Cardiac Society)》1994,72(2):112-118
OBJECTIVE--To assess the diagnostic value at admission of creatine kinase MB mass concentration, alone or in combination with electrocardiographic changes, in suspected myocardial infarction. DESIGN--Prospective study of all consecutive patients admitted within 12 hours after onset of chest pain to a coronary care unit for evaluation of suspected myocardial infarction. SETTING--Large regional hospital. PATIENTS--In 297 patients creatine kinase and creatine kinase MB activities and creatine kinase MB mass concentration were determined. Myocardial infarction according to the criteria of the World Health Organisation was diagnosed in 154 patients and excluded in 143 patients (including 70 with unstable angina pectoris). RESULTS--Sensitivity/specificity for creatine kinase MB mass concentration in patients admitted within 4 hours and 4-12 hours after onset of chest pain were 45%/94% and 76%/79% respectively. Corresponding values for creatine kinase activity were 20%/89% and 59%/83%, and for creatine kinase MB activity 16%/87% and 53%/87%. Raised creatine kinase MB mass concentration was seen in 17% of patients with unstable angina pectoris. Stepwise logistic regression analysis showed that independent predictors of acute myocardial infarction in patients admitted within 4 hours after onset of chest pain were electrocardiographic changes and creatine kinase MB mass concentration on admission; in patients admitted 4-12 hours after the onset of pain independent predictors were electrocardiographic changes and creatine kinase MB mass concentration and activity. CONCLUSION--Creatine kinase MB mass concentration is a more sensitive marker for myocardial infarction than the activity of creatine kinase and its MB isoenzyme. Electrocardiographic changes on admission in combination with creatine kinase MB mass concentration (instead of creatine kinase and creatine kinase MB activities) are best in diagnosing myocardial infarction. 相似文献
10.
Mononuclear cell proliferative response to IL-2and functional response to IL-4 is disturbed in IBD. Theaim of the present study was to verify whether alteredexpression of the common gamma chain(c) might contribute to this abnormality. Thec expression on peripheral bloodmononuclear cells was analyzed by flow cytometry usinga monoclonal antibody to c. IL-4binding in association with cexpression was assessed using biotinylated IL-4 intwo-color flow cytometry. Mean fluorescence of etaexpression was significantly decreased in active CDpatients as compared to inactive CD and healthy controls(P < 0.05). Cell activation as a possible reason forc down-regulation was confirmed usingthe in vitro stimulated donor cells. In the same systemit was shown that down-regulation of cis accompanied by decreased numbers of IL-4 binding cells. Inconclusion, a decreased expression of thec on peripheral blood mononuclearcells from CD patients with active disease might have animportant pathogenetic significance in this chronicintestinal disorder. 相似文献