New method for threshold voltage extraction of high-voltage MOSFETs based on gate-to-drain capacitance measurement

This letter reports on the extraction of the threshold voltage of laterally diffused MOS transistors. A clear analysis of the device physics is performed, highlighting the correlation between the change of the electron charge distribution along the channel and the device capacitance variations when the gate voltage is swept. Using numerical simulations, it is shown that the peak of the gate-to-drain capacitance is related to the transition of the surface from weak to moderate inversion in the intrinsic MOS transistor at the location of the maximum doping concentration, which corresponds to the threshold voltage of the device according to the MOS theory. Comparison between conventional I/sub D///spl radic/g/sub m/ extraction and the new proposed capacitance peak method is performed on both technology computer-aided design simulations and measurements in order to confirm the new experimental technique and related theory.     

Validation of the performance of a GMO multiplex screening assay based on microarray detection

A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct hybridisation of the amplicons on a predefined microarray (DualChip^® GMO, Eppendorf, Germany). The validation was performed within the framework of a European project (Co-Extra, contract no 007158) and in collaboration with 12 laboratories specialised in GMO detection. The present study reports the strategy and the results of an ISO complying validation of the method carried out through an inter-laboratory study. Sets of blind samples were provided consisting of DNA reference materials covering all the elements detectable by specific probes present on the array. The GMO concentrations varied from 1% down to 0.045%. In addition, a mixture of two GMO events (0.1% RRS diluted in 100% TOPAS19/2) was incorporated in the study to test the robustness of the assay in extreme conditions. Data were processed according to ISO 5725 standard. The method was evaluated with predefined performance criteria with respect to the EC CRL method acceptance criteria. The overall method performance met the acceptance criteria; in particular, the results showed that the method is suitable for the detection of the different target elements at 0.1% concentration of GMO with a 95% accuracy rate. This collaborative trial showed that the method can be considered as fit for the purpose of screening with respect to its intra- and inter-laboratory accuracy. The results demonstrated the validity of combining multiplex PCR with array detection as provided by the DualChip^® GMO (Eppendorf, Germany) for the screening of GMO. The results showed that the technology is robust, practical and suitable as a screening tool.     

Statistical Evaluation of Real-Time PCR Protocols Applied to Quantify Genetically Modified Maize

Real-time PCR (RTi-PCR) is the technique of choice for event-specific quantification of genetically modified organisms (GMOs) by determining the amount of event with respect to a species-specific reference gene. Reference genes can be amplified from the genome extracted from Certified Reference Materials (CRMs) or from ad hoc designed plasmids. In the present study, we statistically evaluate the performance of RTi-PCR protocols for GM maize MON810 event by using both genomic DNA from conventional CRMs and a plasmid containing sequences representative of four maize species-specific reference genes. The significance of simple and interaction effects of several variables included in the experimental design on DNA quantification methods and RTi-PCR were evaluated and discussed. Statistically significant differences on Ct values may have an impact on the GMOs quantification and consequently on the compliance of GM quantification-established legal thresholds. Our results confirm the reliability of the plasmid as alternative calibrant for the calculation of GMOs copy number.     

A Multi-Disciplinary Contribution to the Provenance Determination of Ancient Greek and Roman Marble Artifacts

The problem of determining the provenance of the white marble used for antique artifacts has been tackled in different ways. During the past decade it became clear that a multimethod approach is required to solve the problem. In addition, reliable reference material, i.e., samples collected at the ancient quarry sites in an archaeologically and geologically justified manner, turned out to be a prerequisite for reliable provenance determination. A multimethod approach based on petrography, stable isotope analysis of O and C, and trace element analysis yields complementary information. These methods were applied to the same set of 225 quarry samples. When processed with powerful statistical methods, the data thus obtained allowed all major types of marble used in antiquity to be distinguished. Thus, most problems concerning the provenance of artifacts can be solved. This approach was successfully applied to the provenance determination of about 75 artifacts from different museums in Europe and the USA.     

Can dual-headed 18F-FDG SPET imaging reliably supersede PET in clinical oncology? A comparative study in lung and gastrointestinal tract cancer

In this study, we prospectively compared the sensitivity of PET and planar SPET (collimated gamma camera) 18F-FDG imaging in patients with lung and gastrointestinal tract cancer and analysed their respective impact on patient management. Twenty-eight patients with lung cancer and 14 with gastro-intestinal tract tumours were scanned on the same day with a PET and a collimated planar SPET gamma camera. The planar SPET procedure consisted of whole-body planar views and a tomographic acquisition centred over the torso or the abdomen, with the total imaging time within the same range as the whole-body PET procedure. The staging of lung cancer patients was accurate in 86% with PET and 64% with planar SPET. Planar SPET would have led to inappropriate therapeutic decisions in 8 of 28 patients, mainly due to undetected distant metastases. In patients with suspected gastrointestinal tract cancer, planar SPET identified 7 of 15 (47%) proven tumour sites, whereas PET identified 14 of 15 (93%). Our results suggest that collimated planar SPET cameras are not a substitute for dedicated PET scanners. The sensitivity for the detection of tumours is unacceptably low and can impair patient management. The use of multiple tomographic acquisitions could improve the sensitivity but would require a longer scanning time.     

Use of transportable test stations for measuring satellite coverage dependent parameters during IOT campaigns

The in-orbit tests of the satellite coverage dependent parameters require the rotation of the satellite around its pitch and roll axes in order to ‘bring’ the various coverage locations onto one fixed IOT station. Alternatively, several transportable test stations can be located at different sites in the coverage area in order to measure the satellite performances on site. Although the second technique does not provide enough measurement points to reconstruct the complete coverage of shaped beam antennas, it presents several advantages for which it is still used by EUTELSAT for the IOTs of its satellites in conjunction with measurements based on the satellite attitude movements. In particular, the transportable test stations are used to test the stability of satellite EIRP and IPFD at the edges of the coverage areas, where the performances are more sensitive to the pointing errors induced by thermal deformation or satellite attitude changes. This paper presents the common experience gained by EUTELSAT and ESA in testing the coverage dependent parameters of the first and second generation of EUTELSAT satellites. In particular, the paper deals with the use of the transportable test stations during the EUTELSAT II F1 to F3 acceptance tests and with the test campaigns performed at several sites in the eastern European countries. In the first case, the transportable test stations were used in conjunction with satellite rotations around the pitch axis in order to measure the coverage dependent parameters along several cuts parallel to the equatorial plane at different roll angles. In the second case, the transportable test stations were used to periodically repeat the EIRP measurements of many satellite channels over several hours in order to test the EIRP stability of the EUTELSAT II satellites. For both cases, the paper presents a synthesis of the most significant IOT results together with a comparison between these results and the values of the coverage dependent parameters predicted on the basis of the on-ground tests.     

Precise Measurement of Isotope Ratios with a Double-Focusing Magnetic Sector ICP Mass Spectrometer

The potential of a commercially available double-focusing magnetic sector ICP mass spectrometer (Element, Finnigan MAT, Bremen, Germany) for precise isotope ratio measurement at the low-resolution setting (R = 300) was evaluated. Optimization of scanning conditions led to a relative standard deviation for a set of 10 consecutive 2 min measurements of ～0.1% ((206)Pb(+)/(207)Pb(+)) at signal intensities of ～200?000 counts/s (peak height). This compares favorably with the best values ever reported for quadrupole ICPMS and barely exceeds the theoretical value (counting statistics). Increasing the signal intensity to values ≥500?000 counts/s (peak height) resulted in a further reduction of the RSDs obtained (for both (25)Mg(+)/(26)Mg(+) and (206)Pb(+)/(207)Pb(+)) to typically 0.04%. These figures are remarkably better than those reported for commercially available quadrupole ICPMS systems. This improvement significantly reduces the difference between isotope ratio precision of ICPMS on one hand and those of thermal ionization mass spectrometry and plasma source multiple collector mass spectrometry on the other.