单克隆抗体ELISA检测犊牛粪便中冠状病毒抗原

用两株单克隆抗体混合后包被ＥＬＬＩＳＡ微孔板，加牛冠状病毒抗原，加经白陶土处理过的兔抗牛冠状病毒免血清，再加上辣根氧化物酶标记的羊抗兔ＩｇＧ抗体，加底物质显色间接ＥＬＩＳＡ检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液，其敏感度高出血凝试验（ＨＡ）８倍，从武汉市附近３个奶牛场采集犊牛腹泻粪便１０５份，用单抗ＥＬＩＳＡ检测牛冠状病毒抗原，共检出阳性３６份，并与血凝及血凝抑制试验，     

NM－1号抗旱剂的研究

试验结果表明谷子在—１３．９Ｐａ的负压下发芽率只有２４．０％，在—１６．７３Ｐａ时发芽率为１６．３％。用０．５％的ＮＭ—１号抗旱剂包衣后，在—１６．７３Ｐａ的负压下发芽率可达６５．６％，比对照提高４９．３％。同０．５％的ＮＭ—１抗旱剂在大豆现蕾期喷施叶面１次，相对收获株率为１１５．３８％，相对千粒量为１０７．７％，增产率为４０，２７％。玉米在６叶期喷施０．５％的抗旱剂后，饱和亏减少４．０９ｇ，是对照的４３．８１％；干物质积累比对照快６７．１９％，蒸腾系数减少８．３６ｇ。     

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.     

压力实时监测法在管道泄漏检测中的应用

检测输油管道漏油的方法很多，介绍了通过压力实时监测确定输油管道漏油点方法，以及实际应用情况。实践证明，通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。     

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.     

一种快速分析香蕉果实采后mRNA水平变化的新方法

采用SMARTPCRcDNA合成与计算机软件相结合的方法,快速分析香蕉果实采后mRNA水平的变化。在28℃条件下分别将处于同株同穗同梳相邻位置的香蕉果实放置24、48、72h。应用这种方法对其总mRNA水平进行了分析,结果表明,香蕉采后48h时,与采后0h相比,mRNA水平最高。该方法由于使用了计算机软件TheScionImage,可对cDNA电泳图进行数字化分析,具有用材少,敏感性高,快捷方便等特点,特别适用于对较多样品进行分析。     

改进芳草A系原种产卵性的试验

本试验采取母蛾延迟交配，拆对后冷藏、改变交配时间和产卵室保护温度等技术措施来探讨改善芳草A系原种产卵性的方法。结果表明，这些措施对产卵性能的改进有一定的效应，但由于性状间存在相关关系，对某一性状的促进效应往往同时也对另一产卵性状产生负向影响。     

红虹木业，“顶端优势”之我见

《国际木业》：上海红虹木业有限公司作为一家拥有悠久历史的家族企业，自从事木材行业至今已发展为在北美、欧洲、东南亚等地区都有力量分布的规模供应商，整个过程中企业对自己的理念和战略有怎样的发展？     

深基坑锻锤基础冬期施工

在冬期施工，对于土方开挖、钢筋绑扎、人员的通行、砼的浇铸、砼的温度控制，基坑的保温等都带来一定困难，为此提出了深基坑锻锤基础才是最佳实践应用。     

黑杨无性系日CO2固定总量的测定及与苗木生产潜势的相关性研究

通过对不同基因型黑杨无性系植株净光合速率日变化曲线与光合主体叶面积在时间和光强上进行累计积分来计算植株日CO2固定总量P。由于净光合速率日变化曲线不易准确测得，故以光响应曲线和光强的日变化来推测净光合速率日变化。对P值和生物量的相关性研究表明，二者的相关性很高。且认为可以用计算出的P值作为评估苗木生产潜势的指标之一，这为育种中生产力的早期选择提供了有力的证据。