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A novel technique of immobilizing indicator dyes by electrostatic adsorption and covalent bonding to fabricate optical sensors
was developed. 3-Amino-9-ethylcarbazole (AEC) was attached to the outmost surface of quartz glass slide via aminosilanizing
the slide, crosslinking chitosan, adsorbing Au nanoparticle, self-assembling HS(CH2)11OH, and coupling AEC. Thus, an AEC-immobilized optical sensor was obtained. The sensor exhibits a wide linear response range
from 7.0×10−7 to 1.0×10−4 mol/L and a correlation coefficient of 0.995 9 for the detection of 2-nitrophenol. The detection limit and response time
of the sensor are 1.0×10−7 mol/L and less than 10 s, respectively. The fluorescence intensity of the used sensor can be restored to the blank value
by simply rinsing with blank buffer. A very effective matrix for immobilizing indicator dye is provided by the proposed technique,
which is adaptable to other indicator dyes with amino groups besides AEC.
Foundation item: Project(20775010) supported by the National Natural Science Foundation of China; Project(208095) supported by the Key Project
of Ministry of Education, China; Project(07A006) supported by the Scientific Research Fund of Hunan Provincial Education Department,
China; Project(07JJ3020) supported by Hunan Provincial Natural Science Foundation of China 相似文献
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研究能有效降低抗体的体内脱碘的标记方法。碘标记N-琥珀酰亚胺-3-(三正丁基锡)苯甲酸酯(ATE)前体,得到N-琥珀酰亚胺-3-碘[125I]苯甲酸酯(S125IB),分别与人IgG和抗人肝癌单抗(Hepama-1)进行偶联,探索最佳标记条件,并测定标记物的稳定性和生物活性,研究直接标记和间接标记的Hepama-1在正常小鼠体内的生物学分布。结果表明,用N-氯代琥珀酰亚胺(NCS)法125I标记ATE前体,在ATE用量为25~100μg、NCS用量为10~20μg、磷酸盐缓冲溶液(PBS)用量为10~20μL、反应时间为5 min时,标记率大于95%;S125IB和人IgG的偶联率最高可达75%,偶联产物稳定性、生物活性良好;与Hepama-1偶联率可达75%以上。生物分布的对比实验证明,1,3,4,6-四氯-3α,6α-二苯甘脲(Iodogen)直接标记的Hepama-1在甲状腺的放射性摄取率(脱碘显示)最高是S125IB间接标记的Hepama-1的87.9倍。这说明以ATE为前体的放射性碘间接标记蛋白质方法与传统的碘直接标记方法相比较,在解决体内严重脱碘问题上具有明显的优越性。 相似文献
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Niobium(V) ethoxide (Nb(OEt)5) was synthesized by electrochemical reaction of ethanol with niobium plate as the sacrificial anode, stainless steel as the
cathode and tetraethylammonium chloride (TEAC) as the conductive additive. The condensates were isolated by vacuum distillation
under 5 kPa. The product was characterized by Fourier transform infrared (FT-IR) spectra, Raman spectra and nuclear magnetic
resonance (NMR) spectra. The results indicate that the product is niobium ethoxide. Thermal properties of niobium ethoxide
were analysed by TG/DTG. Vapour pressure was calculated from the Langmuir equation and the enthalpy of vaporization was calculated
from the vapour pressure-temperature data using the Clausius-Clapeyron equation. The concentrations of impurity metallic elements
in the sample were detected by ICP-MS. It is shown that the purity can reach 99.997%. The volatility and purity of the niobium
ethoxide ensure that it could be a good precursor for chemical vapor deposition and atomic layer deposition of niobium oxide
layers. 相似文献
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铼[188Re]羰基化合物标记含RGD的环肽及其生物分布 总被引:3,自引:0,他引:3
以fac-[188Re(CO)3(H2O)3] 为前体标记了含RGD的环形多肽,并对其在普通小鼠和荷S180肉瘤小鼠体内的生物分布作了初步研究.铼[188Re]羰基化合物标记含RGD环肽标记方法简单易行,75℃反应30min,标记率在90%以上.不会引起红细胞的溶血和凝聚现象,普通小鼠和荷S180肉瘤小鼠的生物分布实验表明,标记肽主要通过泌尿系统和肝胆代谢两个途径清除,1、4、24和48h肿瘤/肌肉比分别为:(4.4075±0.1139)、(5.1510±0.5375)、(4.4727±0.4895)和(4.8788±3.16). 相似文献
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