BET Inhibition Upregulates SIRT1 and Alleviates Inflammatory Responses

Control of histone acetylation is a part of the epigenetic mechanism that regulates gene expression and chromatin architecture. The members of the bromodomain and extra terminal domain (BET) protein family are a group of epigenetic readers that recognize histone acetylation, whereas histone deacetyl‐ ases such as sirtuin 1 (SIRT1) function as epigenetic erasers. We observed that BET inhibition by the specific inhibitor JQ1 upregulated SIRT1 expression and activated SIRT1. Moreover, we observed that BET inhibition functionally reversed the pro‐inflammatory effect of SIRT1 inhibition in a cellular lung disease model. SIRT1 activation is desirable in many age‐related, metabolic and inflammatory diseases; our results suggest that BET protein inhibition would be beneficial in treatment of those conditions. Most importantly, our findings demonstrate a novel mechanism of SIRT1 activation by inhibition of the BET proteins.     

Preparation and characterisation of α-methylstyrene–butadiene latexes for paper coating applications

The purpose of this work is to demonstrate how α-methylstyrene (AMS) can replace styrene in preparing styrene–butadiene (SB) type latexes and to compare the properties of the paper coating of the prepared α-methylstyrene–butadiene emulsion with the commercial styrene–butadiene latex reference sample. A lot of work is nowadays being conducted on different biorefinery concepts replacing fossil oil with biomass based raw materials due to the expected rise of the fossil oil cost. Aromatics can in principle be produced from renewable raw materials, such as lignin, sugars and terpenes for example. The potential methods include thermochemical conversions, catalytic fast pyrolysis, metabolic engineering, catalytic aromatisation and dehydrogenation among others. Terpenes, such as α-limonene and pinene, are possible sources of aromatics, and they can indeed be catalytically converted to p-cymene. Industrial hydrodealkylation and disproportionation processes developed by major petrochemical companies can further convert p-cymene to BTX aromatics or simultaneously dehydrogenate the alkyl chain of p-cymene to styrenic monomers such as α-methylstyrene. Based on the measured paper properties for uncalendered and calendered coated samples, AMS proved to be adequate to replace the oil based styrene in commercial reference SB latexes. Even though the emulsion polymerisation for the α-methylstyrene–butadiene latex was not optimised, almost all tested properties were at least equally good as in the commercial reference sample. α-Methylstyrene containing coating colours had slightly higher viscosity than the other coating colours. Coating colours containing α-methylstyrene seems to have an improved water retention compared to the commercial reference styrene–butadiene latex coating colour and the laboratory prepared styrene–butadiene coating colour. The paper coated with the commercial reference latex containing coating colour was less porous than the other coated papers. Despite of that, both dry and wet surface strength were at least equally good as in the case of the commercial reference latex. The results are promising when thinking of the future development of the bio-based latexes.     

Physical solvent extraction of levulinic acid from dilute aqueous solution with 2-methyltetrahydrofuran

The results of the present investigation reveal that 2-methyltetrahydrofuran is a potential solvent for the extraction of levulinic acid from dilute aqueous solution. This conclusion is based on the relatively high values of distribution coefficient K_d (1.3-1.6) measured for the system of levulinic acid – water – 2-methyltetrahydrofuran at temperatures 298.2 K, 313.2 K, and 328.2 K, as well as encouraging performance of the continuous counter current Kühni column. The results give reason to believe, that 2-methyltetrahydrofuran can be considered for the extraction of other low molecular weight acids, such as formic or lactic acid, as well.     

Strength retention of drawn self-reinforced polyglycolide rods and fixation properties of the distal femoral osteotomies with these rods. An experimental study on rats

Drawn self-reinforced polyglycolide (SR-PGA) rods, Ø 2 mm and 26 mm long, were implanted in the dorsal subcutaneus tissue of 16 rats. Osteotomies of the distal femur were fixed with SR-PGA rods (2 mm by 15 mm) in another 38 rats. The follow-up times varied from one week to one year. After sacrifice, three-point bending and shear tests were performed for subcutaneously placed rods. Radiological, histological, histomorphometrical, microradiographic, and oxytetracycline-fluorescence studies of osteotomized and intact control femora were also performed. At three weeks the flexural strength of the rods was 50% of the initial value, and the flexural modulus was 46% of the initial value. Five osteotomy specimens had to be excluded due to dislocation or non-union. One of the 33 evaluated osteotomy specimens showed signs of postoperative infection. Thirty-two osteotomies healed uneventfully. No gross signs of inflammatory or foreign-body reaction were observed. The amount of osteoid surface and active osteoid formation surface reached their highest value in the histomorphometrical analysis at 24 weeks. The present investigation demonstrated that the mechanical strength and fixation properties of the drawn SR-PGA rods are suitable for fixation of cancellous bone osteotomies in rats. The present article is the first report on the successful application of drawn SR-PGA rods for fixation of cancellous bone osteotomies.     

Comparison of simulators for variable‐speed wind turbine transient analysis

This paper presents a comparison of three variable‐speed wind turbine simulators used for a 2 MW wind turbine short‐term transient behaviour study during a symmetrical network disturbance. The simulator with doubly fed induction generator (DFIG) analytical model, the simulator with a finite element method (FEM) DFIG model and the wind turbine simulator with an analytical model of DFIG are compared. The comparison of the simulation results shows the influence of the different modelling approaches on the short‐term transient simulation accuracy. Copyright © 2005 John Wiley & Sons, Ltd.     

Genetic Variability in DNA Repair Proteins in Age-Related Macular Degeneration

The pathogenesis of age-related macular degeneration (AMD) is complex and involves interactions between environmental and genetic factors, with oxidative stress playing an important role inducing damage in biomolecules, including DNA. Therefore, genetic variability in the components of DNA repair systems may influence the ability of the cell to cope with oxidative stress and in this way contribute to the pathogenesis of AMD. However, few reports have been published on this subject so far. We demonstrated that the c.977C>G polymorphism (rs1052133) in the hOGG1 gene and the c.972G>C polymorphism (rs3219489) in the MUTYH gene, the products of which play important roles in the repair of oxidatively damaged DNA, might be associated with the risk of AMD. Oxidative stress may promote misincorporation of uracil into DNA, where it is targeted by several DNA glycosylases. We observed that the g.4235T>C (rs2337395) and c.–32A>G (rs3087404) polymorphisms in two genes encoding such glycosylases, UNG and SMUG1, respectively, could be associated with the occurrence of AMD. Polymorphisms in some other DNA repair genes, including XPD (ERCC2), XRCC1 and ERCC6 (CSB) have also been reported to be associated with AMD. These data confirm the importance of the cellular reaction to DNA damage, and this may be influenced by variability in DNA repair genes, in AMD pathogenesis.     

Performance evaluation of an automation system — determination of a benchmzrk process

An average “real” process is determined from 55 processes automated by an automation system. This a first step in developing realistic benchmark processes for making objective evaluation tests between the performance of different automation systems. The most important properties of the average process that are independent of the automation system used and that are important performance indicators are presented in the paper.The real process, representing the statistical mean of the process data best, is a papermachine with a pulp making department. The process has 141 analog inputs, 50 binary inputs, 90 analog outputs, 63 binary outputs, 77 motor controls, 8 motor value controls, 308 process interfaces, and 165 displays.     

Detection of combined faults in induction machines with stator parallel branches through the DWT of the startup current

The main objective of this paper is to diagnose the presence of combined faults in induction machines. For this purpose, a methodology based on the application of the Discrete Wavelet Transform (DWT) to the stator startup current is used. This approach was applied in previous works with success to the diagnosis of rotor asymmetries and mixed eccentricities in motors with different sizes and conditions. However, as most of the diagnosis methods hitherto developed, the application of the proposed approach was circumscribed to situations in which a single fault was present in the machine. In addition, the influence of other phenomena such as load torque oscillations or voltage fluctuations was studied, but without considering the combination of these phenomena and the fault in the machine. This work is intended, first, to apply the proposed transient-based methodology to several cases in which different faults (rotor asymmetries, mixed eccentricities and inter-turn and inter-coil stator short-circuits) are simultaneously present in the machine and, second, to apply it to cases regarding faults combined with other phenomena making difficult the diagnosis, such as load torque oscillations. Interesting considerations regarding the preponderance of the effects of some of the faults are also done in the paper. The application of the methodology is focused on induction machines with stator parallel branches; in this sense, the suitability of the use either of the phase current or of the branch current for the diagnosis of each particular fault is analysed. The results look promising with regard to the validity of the methodology for the reliable discrimination of simultaneous electromechanical faults and the diagnosis of faults combined with other phenomena.     

Hysteresis model for finite-element analysis of permanent-magnet demagnetization in a large synchronous motor under a fault condition

This paper describes a rare-earth-transition metal alloy permanent-magnet (Nd/sub 2/Fe/sub 14/B) hysteretic behavior model within finite-element analysis. The present work analyzes the demagnetization states of permanent magnets during fault conditions in a permanent-magnet synchronous motor and characterizes the ability of the motor to sustain the designed rated outputs after the fault conditions occur.     

Low-order parametric force model for a salient-pole synchronous machine with eccentric rotor

In this paper, a low-order parametric force model to represent the electromagnetic force on an eccentric rotor of a salient-pole synchronous machine was developed and verified. The force model with parameters estimated from the results of impulse method showed excellent performance. The proposed model allowed quick and accurate calculation of the electromagnetic force at the desired whirling frequency values and provided an opportunity to effectively combine the electromagnetic and mechanical analyses of electrical machines. Besides, results presented in this paper also verified the suitability of computationally efficient impulse method for the analysis of a synchronous machine.