鲁山县集雨工程建设的做法与经验

鲁山县为了解决丘陵区干旱缺水问题,在田间地头大量建设集雨工程,拦蓄地表径流,涝时蓄水,旱时灌溉,有效地解决了丘陵区的水源问题。介绍了主要做法和经验。     

标准体积管重复性的计算

介绍了体积管标准体积的计算方法,分析和比较了标准JJG 209～1980、JJG 209-1994和API MPMS 12.2.4-1997等关于体积管重复性的计算方法及相关规定,使用不同的计算方法计算了体积管的重复性.     

Expression and modulation of connective tissue growth factor in renal interstitial fibrosis

CTGF, a member of the CCN family of immediate early genes, is a recently discovered profibrotic growth factor, which is involved in many pathophysiologic procedures. CTGF acts as a downstream effector of TGF-β acting on interstitial cells to enhance the progression of fibrotic renal diseases. It has been shown that CTGF gene expression can be induced or blocked by some kinds of cytokine and drugs. It is an interesting candidate target for future intervention strategies of renal interstitial fibrosis.     

The rates of maize growth and lignin biosynthesis change after root-applied chalcone

The effects of root-applied chalcone at 0.15 mmol L⁻¹ on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis.     

母猪卧向行为学的观察

本文通过2个试验对母猪在分娩圈和分娩栏中的卧向行为进行了观察,试验一中, 选择10头大白(Yorkshire)经产母猪作为观察对象,试验二中分别选择10头大白(Yorkshire) 经产母猪和10头长白(Landrace)经产母猪作为观察对象。观察采用瞬时记录方法,每周观察 三次,隔日观察,每天上午、下午各观察一次,每次3 h,每次观察间隔5 min。观察中发现,母猪 卧向以向外为主。分娩栏(四周是栏杆)中的母猪以选择卧向外(向北)为主,其次是卧向内(向 南),卧向左和卧向右最少且差异不显著,上栏前和下栏后差异不显著。分娩圈(四周是墙壁)中 母猪以选择卧向外为主,卧向内最少,卧向左和卧向右差异不显著。长白母猪比大白母猪选择 卧向外的多,妊娠阶段比哺乳阶段选择卧向外的多。     

利用RT-PCR与PVP-ELISA检测水稻瘤矮病单头叶蝉

 目前国内外对水稻瘤矮病毒(Rice gall dwarf virus,RGDV)的研究主要集中在其核衣壳蛋白和外衣壳蛋白的性质、结构及其与介体的亲和性、与介体传毒能力的关系等方面,本室在已有室内检测研究的基础上,利用RT-PCR和PVP-ELISA法对田间带毒叶蝉进行检测,并对2种方法的检测结果进行了比较。     

Role of potassium channels in the regulation of intracellular free Ca2+ concentration of pulmonary artery smooth muscle cells in rats

AIM: To investigate the role of potassium channels in the regulation of intracellular free calcium concentration ( [Ca²⁺]_i) of pulmonary artery smooth muscle cells (PASMCs) in rats. METHODS: The fluorescence Ca²⁺ indicator Fura-2/AM was used to observe [Ca²⁺]_i of rat PASMCs in normal and chronic hypoxic condition. The influences of potassium channels on PASMCs proliferation were assessed by MTT assay. RESULTS: 1. In normoxic condition, [Ca²⁺]_i was (156.91±8.60) nmol/L, and in hypoxic condition, [Ca²⁺]_i was (294.01±16.81) nmol/L. 2. In normoxic condition, the voltage-dependent K⁺-channel antagonist 4-aminopyridine (4AP), but not the Ca²⁺-activated K⁺-channel antagonist tetraethylammonium (TEA) and the ATP-sensitive K⁺-channel antagonist glibenclamide (Glib) increased [Ca²⁺]_i. 3. In hypoxic condition, 4AP and TEA caused the rise in [Ca²⁺]_i , but Glib had no effect on [Ca²⁺]_i. 4. MTT assay showed that 4AP increased the value of absorbing light degree (A value) in normoxic and hypoxic condition (0.582±0.062,0.873±0.043,respectively, P＜0.01), TEA increased A value only in hypoxic condition, and Glib had no effect on the proliferation of PASMCs. CONCLUSIONS: K_V plays an important role in the regulation of [Ca²⁺]_i and proliferation of PASMCs. K_Ca serves as distinct responsive roles in the regulation of proliferation of PASMCs in hypoxic condition. K_ATP has no effect on [Ca²⁺]_i and proliferation of PASMCs in normoxic and hypoxic conditions.     

湖南速生材杉木浸渍改性工艺研究

选取湖南省各地速生材杉木为试样材料,根据速生材杉木早晚材的硬度、年轮宽度、密度等材性差异的特点,在保证水溶性改性药剂完全渗入木材的同时,也保证试材在较高压力下不被压溃。研究确定了真空-叠压法浸渍改性工艺,整个浸溃过程能在2．5～3．5h内完成。     

白菜型油菜srb多室性状的遗传分析与分子鉴定

油菜多室角果是一种高产相关性状,本研究对桑日白油菜(srb)多室性状的遗传调控机制进行研究。性状分析表明,该突变体具有稳定的多室角果表型,单株多室角果比例为94.7%～100.0%,每角果平均3.5个心皮。遗传上srb突变体中的多室性状受1对隐性核基因控制。比较测序分析发现, srb中BrCLV3基因的CLE motif中存在一种新的单核苷酸突变(C/G),可导致其保守结构域的第12位组氨酸突变为天冬氨酸,将该位点命名为Brclv3Asp12。利用SNP标记进行分离群体的鉴定,证实Brclv3Asp12中的C/G单核苷酸变异与多室表型共分离。转基因互补测验和体外多肽的处理试验进一步证实,该材料中控制多室性状位点Brclv3_Asp12突变导致了CLV3多肽活性的减弱,是形成多室角果性状的原因。本研究初步阐明了白菜型油菜srb多室性状形成的机制。