一种简易的抗疟药物初筛方法

为了改进抗疟药物的初筛方法,以适应来源有限的药品和减少工作量,将拟试药物混入羊毛脂和石蜡油制成的乳剂中,再作小鼠腹腔注射.用已知有效药物作比较和同位素示踪方法证明,乳剂可起缓释作用,一次注射不仅用药量减少,而且节约人力。     

几株抗血吸虫单克隆抗体细胞株的建立与特性鉴定

为了对血吸虫病的免疫诊断和免疫预防提供有价值的单克隆抗体,通过2次骨髓瘤细胞与感染日本血吸虫的小鼠脾细胞间的融合试验和多次筛选以及克隆化,建立了4株分泌单克隆抗体的细胞株。其中2株的靶抗原为血吸虫成虫肠道;1株为成虫表面膜;另一株为成虫的肌细胞或纤维细胞。免疫球蛋白亚类鉴定表明前3株均为IgG_1。除了SM 48-2对虫卵呈弱反应外,未见对血吸虫虫卵、尾蚴、童虫有阳性反应;亦未见对曼氏血吸虫、肺吸虫、华枝睾吸虫、疟原虫及利什曼原虫有阳性反应。这几株细胞已在体外培养下稳定地分泌特异性抗体达6个月之久。     

用间接免疫荧光抗体法观察日本血吸虫体表cAMP的分布

用合成cAMP-BSA作抗原制得兔抗血清,然后用羊抗兔IgG荧光素作标记观察了日本血吸虫体表cAMP的分布。结果表明雄虫体表cAMP主要分布于吸盘及口腔的边缘,其他部位呈散在性分布。雌虫则呈较均匀的小点状密集分布。     

氟乙酸类化合物杀灭日本血吸虫机理的研究

本文研究了小鼠服氟乙酸类化合物后体内血吸虫基本生化成分的变化以及虫体柠檬酸积聚与虫肝移的关系。结果表明小鼠连续给服氟乙酸类药物4天中,除脂类含量的变化较小外,虫体的体重、糖原含量、     

血吸虫钙调蛋白与吡喹酮杀虫作用机理

提取了日本血吸虫成虫之钙调蛋白制成 DNS—虫钙调蛋白,利用它本身的荧光及加入荧光探针,观察了吡喹酮对其蛋白构象的影响,从而表明在钙离子存在下吡喹酮可与虫钙调蛋白发生结合。对动物钙调蛋白具有亲和性的三氟拉嗪、氯丙嗪及异博定等在体外具有吡喹酮相似的杀虫效果,但在小鼠体内的效果较差。作者认为吡喹酮的杀虫作用是由于吡喹酮使血吸虫的钙调蛋白构象发生固定化,从而导致了一系列代谢系统失调的结果。     

酮替芬治疗猴疟的研究

酮替芬以0.7～20 mg/kg×3天治疗食蟹猴疟证明有明显疗效,无性体与配子体分别于93～102.5 h及102.5～120 h转阴,作用较氯喹慢。对组织期实验证明无效。酮替芬以抗过敏临床治疗剂量0.1 mg/kg合并周效磺胺5 mg/kg连用3天,无性体与配子体分别于79.5 h及153 h消失,表明两药合用有协同作用。使用上述各种剂量未发现嗜睡等不良反应。追踪观察30天,仅酮替芬5 mg/kg×3天治疗2只猴中1只及1 mg/kg×3天治疗的2只猴出现原虫复燃。本研究结果证明,酮替芬是具有抗疟作用的新型化合物,但其对人类疟疾的抗疟作用尚待研究证实。     

伯氏疟原虫氯喹抗性逆转的实验观察

Objective To search for reverser of chloroquine-resistance in Plasmodium berghei (P.berghei) ANKA. Methods Seventy-two healthy Kunming mice were each infected with chloroquine sensitive (CS) or chloroquine resistance (CR) P. berghei ANKA respectively, then treated with various schedule of reversal agents C-2832 、D-6182 or ketotifen(Ket) respectively with or without co-administration of low dose (5%ED90) of chloroquine (CQ). Schedule 1: mice infected with CS were randomly distributed into 4 groups, 6 mice in each group, 30 min after infection,then treated i.g. with D-6182, C-2832, Ket or 0. 1% gum tragacanth(control) respectively for 5 consecutive days. The parasitemia of each experiment group was then determined by routine microscopic examination on blood smears from the tail blood of each mouse from D1 to D7.Schedule 2:mice infected with CR were randomly distriduted into 8 groups, 6 mice in each group, 3 d after infection, then treated i.g. with D-6182, C-2832, Ket, chloroquine or 0. 1% gum tragacanth (control) with or without co-administration of 12 mg/(kg · d) chloroquine (5% ED90) 2 h after the first administration for 5 consecutive days. The parasitemia of each experiment group was then determined microscopically by examination on blood smears from the tail blood of each mouse from D4 to D7. The reduction rates of each group were calculated and compared between the groups with or without treatment of reverser. Results 1. The parasitemia of mice infected with CS was going up daily from D1 to D4 and reached the peak on D4 in all groups administered with 80 mg/(kg · d) D-6182, 120 mg/(kg · d) C-2832 or 10 mg/(kg · d) Ket for5 d. From D5 the parasitemia kept going up in control group while it kept at the level of D4 in all treated groups. 2. Chloroquine 12 mg/(kg · d) administered i.g. 2 h after administration of C-2832 or D-6182 or Ket for 5 d(D3-D7) could reach 97.77%, 99.28% or 96.73% of reduction rate of parasitemia on D4 and 99.81%, 98.87% or 100.00% on D7 respectively in CR P. berghei infected mice. Conclusion Two compounds of C-2832 and D-6182 exhibited the reversal activity on CQ resistance in CR P. berghei infected mice at the same range of that of well-recognized reverser Ket.     

短棒杆菌菌苗等疫苗交叉抗疟原虫感染的作用

在进行疟疾研究中,曾发现疟原虫难以感染的情况,这是否与其他微生物交叉免疫有关尚不清楚.文献报告百日咳菌苗有增强药物抗疟作用的能力,本文观察了15种上海生物制品研究所生产的人用菌     

酮替芬、赛庚啶及苯噻啶体外抗恶性疟原虫的作用

在体外培养条件下观察了酮替芬、赛庚啶及苯噻啶对恶性疟原虫的抑制作用,其中以酮替芬的效果最佳,5×10~-5M酮替芬可明显抑制疟原虫的发育,使虫数减少并不再能形成裂殖体。作者还用同步化了的疟原虫进一步研究了酮替芬对疟原虫生长环节的抑制作用.