Analysis thrombolysis with anticoagulation treatment for early stage of deep vein thrombosis in the lower extremities

Objective:To explore the effect of thrombolysis with anticoagulation treatment forearly stage of deep vein thrombosis of lower extremity.Methods:The clinical data of 10 patients at the early stage of deep vein thrombosis(DVT)in the lower extremuites treated by thrombolysis with anticoagulation and dispersion drugs were analyzed retrospectively.Results:The thrombolytic effect was significant.After treatment,the deep veins were recanalized without regurgitation in 75.3% of the patients.The total effective rate was 100%.Only three patients had hemorrhagic complication,but none of the patients died.Conclusion:Thrombolysis with anticoagulation treatment is an effective and safe method for DVT at the early stage.     

小儿颅内小脓肿28例临床分析

目的提高对颅内小脓肿的认识,以及时诊断、治疗。方法收集28例住院脑脓肿患儿的临床资料进行分析。结果本组以5-10岁发病率最高。临床表现:本组均无发热,主要疾状为癫癎发作,少数有头痛、恶心、呕吐,无明显神经系统阳性体征。结论　颅内小脓肿由于病变部位、大小、数目、病期、临床表现各异,诊断主要依靠临床表现,CT和抗生素试验性治疗,CT为颅内小脓肿的诊断提供可靠依据。     

纳络酮治疗新生儿缺氧缺血性脑病疗效观察

目的观察早期应用纳络酮治疗新生儿缺氧缺血性脑病的疗效。方法治疗组加用纳络酮注射液按0．03-0．05mg／kg．h的速度稀释后输液泵控速连续静点4小时，每日总量不超过0．4mg／d，连用5-7天。结果治疗组有效率92％，对照组有效率66．7％，两组有显著性差异，P〈0．01。结论纳洛酮治疗缺氧缺血性脑病能提高疗效，改善预后，无毒副作用，值得临床推广。     

骨桥蛋白血清白细胞介素-8与狼疮肾炎的病理临床关系

骨桥蛋白（osteopotin，OPN）与系统性红斑狼疮（SEE）的临床活动有关，白细胞介素8（interleukin-8，IL-8）与狼疮肾炎（Lupus Nephritis，LN）的肾损害关系密切。为了探讨狼疮肾炎（LN）患者肾小管OPN表达和血浆IL-8的浓度及其意义，本研究检测了LN患者的OPN，并分析其表达与病理分型、临床表现和血清IL-8的关系。     

警惕好人缘的“外向孤独症”

近段时间,网上流传一个帖子——“外向孤独症八大症状,你中枪了吗？”这个帖子引起了很大反响,很多网友跟帖,慨叹“躺着也中枪”。     

实时荧光定量PCR检测布鲁杆菌方法的应用

Objective To discuss a real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) wether if can be used to detect Brucella. Methods According to the BCSP31 gene sequences specific for Brucella, one pair of primers and one TaqMan probe were designed. A real-time PCR was developed with the BCSP31 fragments cloned into PMD18-T vector. The standard cure was established and the sensitivity, the species specificity and the stability of the assay were evaluated. The clinical blood specimens were detected by QT-PCR and compared with clinical diagnosis. Results The standard curve was established with the standard template and the relationship between the Ct and the DNA copy number was linear(r=0.999). The sensitivity of the real-time PCR was 5 copies/μl. The sensitivity of the common PCR was 5×102 copies/μl. The sensitivity was about 100 times higher than common PCR. Species specificity of this FQ-PCR assay evaluated using genomic DNA from 6 Bmcella strains and 5 non-Brucella strains and strong fluorescence was detected in all Brucella strains. The CV of intra-assay and inter-assay reproducibility were 0.71%,7.23%, reprectively. Twenty-four specimens from clinical brucellosis cases, 19 showed positive, the positive coincident rate was 79%(19/24). The negative results were obtained for all 31 negative control, and the negative coincident rate was 100%(31/31). Two were positive from all 30 specimens clinically suspected. Conclusions Highly specific, sensitive, repeatable and coincidental with clinic, this FQ-PCR is quite useful for rapid detection of tiny DNA of Brucella in various samples and laboratory diagnosis.     

成人颈椎椎弓根螺钉在儿童腰椎应用的可行性

背景：由于1～3岁幼年儿童椎体发育未完全成熟,各种解剖径线相对较成人小得多,尚无幼儿专用的椎弓根螺钉固定器械,现有能够利用的直径最小的椎弓根螺钉是用于成人颈椎侧块或椎弓根固定的钉棒系统。目的：观察将成人颈椎椎弓根螺钉应用到成年猪颈椎与幼猪腰椎固定后的生物力学对比。方法：将6具完整新鲜成年猪颈段C3～C6脊椎标本和6具完整8周龄新鲜幼猪腰段脊柱标本自椎间盘及关节处离断,游离成单个椎体,共54个椎体108侧椎弓根。按照标准操作将成人颈椎椎弓根螺钉分别安置在成年猪颈椎标本和幼猪腰椎标本的椎弓根上,应用生物力学方法测试螺钉的最大轴向拔出力。结果与结论：颈椎标本最大轴向拔出力高于腰椎标本,但差异无显著性意义（P〉0.05）;L1椎弓根螺钉的拔出力均值明显小于L3椎弓根螺钉的拔出力均值（P〈0.05）;C5椎弓根螺钉的拔出力均值明显大于C3椎弓根螺钉的拔出力均值（P〈0.05）;颈椎和腰椎标的骨密度差异有显著性意义（P〈0.01）,椎体椎弓根力学数值与椎体骨密度之间存在线性正相关。说明取得了成人颈椎椎弓根螺钉在轴向拉力方面适应于幼儿腰椎的初步实验依据。     

关节腔注射右美托咪定对膝关节镜术后镇痛的效果

目的 观察关节腔内注射右美托咪定对膝关节镜术后镇痛的效果.方法 择期行膝关节镜手术患者40例,随机分为生理盐水组（关节腔注射生理盐水20 ml）和右美托咪定组（关节腔注射右美托咪定1 μg/kg加生理盐水配置成20 ml）,每组各20例.于术后1、2、4、6、10、12、24 h时以视觉模拟评分（VAS）评价术后镇痛效果,术后当患者VAS评分≥4时静脉注射氯诺昔康8 mg镇痛,每8小时可重复注射1次.记录术后首次需要氯诺昔康镇痛的时间、术后24 h内氯诺昔康的用量及用药后的不良反应.结果 术后6h内右美托咪定组镇痛效果优于生理盐水组（P＜0.05）,右美托咪定组术后首次需要氯诺昔康镇痛的时间（320±115 min）较生理盐水组（69±46 min）延迟,24 h内总用量（8.7±1.9 mg）低于生理盐水组（16.8±2.5mg）（P＜0.05）.结论 关节腔内注射右美托咪定用于膝关节镜术后镇痛能够产生良好的镇痛效果,延长术后首次需要镇痛药辅助的时间.     

髓样细胞激活受体-2 对术后认知功能障碍调控的 研究进展

术后认知功能障碍（POCD）是一种急性精神功能紊乱状态,伴有认知以及注意力等的损 害,与中枢神经系统炎症密切相关,但其机制仍未探索清楚。TREM2 为髓样细胞2 触发受体,在小胶质 细胞、树突状细胞等多种细胞上表达。DAP12为重要的接头蛋白分子,介导TREM2等受体传递的信号。 TREM2-DAP12是一种特异表达于小胶质细胞上的复合体,具有抗炎和促进小胶质细胞吞噬功能的作用。 现对TREM2-DAP12的结构、生理功能以及对小胶质细胞在POCD的调控作用以及研究进展进行综述。     

孕期防“意外”丈夫险些“被女人”

于竹衡今年32岁,2005年,他从哈尔滨工业大学硕士毕业后,应聘到成都一家颇具规模的国企,认识了同一家公司市场部的卿蕾。由于两人是老乡,又志趣相投,很快就坠入了爱河,2009年五一,他们携手走进了婚姻殿堂。婚后的生活甜蜜而幸福,2009年9月,卿蕾怀孕了!小两口非常激动,赶紧买了很多孕期保养的书