人类ABO血型系统-B抗原单克隆抗体的研制

血库、临床医院、法医以及在血液免疫学、人类遗传学的基础理论研究方面,都迫切需要高特异性、高亲和性(avidity)、高亲和力(affinity)的血型McAb,从80年代初始,国内外研制ABO血型McAb的工作。目前,有些国家已经商品生产,取代了ABO系统     

不同门奇断流术式对门静脉高压症犬食管下段血管再生的影响

Objective To explore the effect on blood vessel regeneration of distal esophagus in ca-nines of portal hypertension with liver cirrhosis after different procedures with paraesophagastrie devascular-ization. Methods Portal hypertension models were produced in canines by subcutaneous injection of 60% CC14 combined with food restriction. Forty eight model canines were randomly divided into 4 groups: group A, traditional paraesophagastrie devascularization;group B, selective paraesophagastric devascularization;group C, paraesophagastric devascularization plus distal esophageal transaction and reanastomosis with sta-pler;group D,control group. Every group had 12 canines which were randomly divided into A1 ,B1 ,C1 ,D1, A2,B2,C2 and D2 groups after the operation. Canines in groups A1 ,B1 ,C1 and D1 were executed at first month after the operation,and those in groups A2, B2, C2 and D2 were executed at the 6th month after the operation. The distal esophagus was segmented when the canines were executed. The upside and downside of the stoma in group C were distinguished and signed as Ca and Cb. Immunohistochemieal methods were used to detect VEGF,CD34 and FVⅧ-Rag,blood vessel-related factors,in lower esophagus. Results The expres-sion levels of VEGF and CD34 MVD,FⅧ-Rag MVD in groups A1 ,B1 and C1 were lower than those in group D1 one month after the operation (P <0.05). All the indices in group B1 were lower than those in groups A1 and Clb,but higher than those in group Cla (P <0.05). The indices in group Cla were lower than those in group C1b (P < 0.05 ). The indices in groups A2 and B2 were higher than those in groups A1 and B1, re-spectively at the 6th month after the operation (P <0.05). They were higer in outer membrane in group C1a than in group C2a (P <0.05). The indices in group C2b were higher than those in group Clb (P<0.05). Conclusion At the first month after operation, the expression of blood vessel-related factors was declined in three different procedures of paraesophagastric devascularization,which indicated that all three different pro-cedures could diminish the vessels in distal esophagus. The effect in group C was most obvious among all groups. At the 6th month after traditional paraesophagastrie devascularization and selective paraesophagastrie devascularization, the expression of blood vessel-related factors in distal esophagus was declined. But there were no significant changes in the expression of blood vessel-related factors in distal esophagus after parae-sophagastric devascularization plus distal esophageal transaction and reanastomosis with stapler. It indicated that the blood vessel regeneration in paraesophagastric devascularization pins distal esophageal transaction and reanastomosis with stapler was inferior to that in other procedures.     

肝动脉化疗栓塞联合血管内皮抑素治疗肝癌的疗效及其对血浆基质金属蛋白酶9、骨桥蛋白的影响

目的评估肝动脉化疗栓塞(TACE)联合血管内皮抑素治疗肝癌的临床疗效,并监测血浆基质金属蛋白酶9(MMP-9)、骨桥蛋白(OPN)的动态变化。方法治疗组20例为TACE联合重组人内皮抑素经动脉灌注,对照组20例为单纯TACE,所有患者检测治疗前1天及术后1、3、7、30、60d的血浆MMP-9、OPN浓度,并观察肿瘤控制情况。结果治疗组疾病控制率(DCR)显著高于对照组(P=0.038),治疗组在术后3、7、30d血浆MMP-9浓度较对照组明显下降(P<0.05),治疗组术后7、30、60d的血浆OPN浓度得到明显抑制(P<0.05)。结论内皮抑素联合TACE治疗肝癌疗效确切,肿瘤新生血管、血浆MMP-9、OPN水平得到明显抑制,减少肿瘤复发及转移机会,值得临床推广。     

4,4′-二异硫氰基芪-2,2′-二磺酸对内质网应激诱导心肌细胞损伤的影响

目的探讨氯离子通道阻滞剂4,4′二异硫氰基芪2,2′-二磺酸(DIDS)对衣霉素诱导心肌细胞损伤以及内质网应激标记性分子糖调节蛋白78(GRP78)和CCAAT增强子结合蛋白样同源蛋白(CHOP)的影响。方法取乳鼠心肌细胞培养后,应用衣霉素构建心肌细胞内质网应激模型,随机分为对照组、衣霉素组、衣霉素+DIDS组、DIDS组。应用100ng/ml衣霉素处理24、48、72、96 h;另用DIDS 25、50、75和100μmol/L处理72 h,噻唑蓝法检测心肌细胞存活率,Western blot法检测GRP 78和CHOP蛋白表达水平。结果与对照组比较,衣霉素组48、72和96 h心肌细胞存活率明显降低(P<0.05);与衣霉素组比较,DIDS 50、75和100μmol/L处理明显增加心肌细胞存活率(P<0.05)。与对照组比较,衣霉素组GRP78和CHOP蛋白表达水平明显升高(P<0.05);与衣霉素组比较,衣霉素+DIDS组GRP78和CHOP表达水平明显下调(P<0.05)。结论 DIDS可以抑制衣霉素诱导的大鼠心肌细胞损伤,其机制可能与拮抗GRP78和CHOP的表达,减轻内质网应激有关。     

冠状动脉溶栓在ST段抬高型心肌梗死中的研究进展

ST段抬高型心肌梗死是常见的心血管急危重症,致死率和致残率高.尽早实现心肌再灌注可有效挽救濒死心肌,改善ST段抬高型心肌梗死患者的预后,冠状动脉内溶栓在实现再灌注过程中发挥重要作用.现从血栓形成的机制、溶栓剂分类、冠状动脉内正向溶栓、血栓抽吸、血栓抽吸联合冠状动脉内溶栓、冠状动脉内逆向溶栓及应用前景等方面展开综述.     

高频电灼治疗慢性泪囊炎的疗效分析

目的：探讨高频电灼治疗慢性泪囊炎所致鼻泪管阻塞的治疗效果。方法：临床治疗46例51只眼慢性泪囊炎病人。随访完整资料36例40只眼。结果：高频电灼法近期(两周)有效率97．5％，长期有效率为(三个月)为77．3％。结论：高频电灼治疗慢性泪囊炎是一种简便可靠，疗效满意的方法。     

烧伤患者气管切开置管全国专家共识(2018版)

大面积烧伤、头面颈部深度烧伤、吸入性损伤等易出现气道水肿、狭窄、梗阻甚至窒息,危及生命,及时行气管切开置管是重要的救治措施,而部分医院存在认识不足与处理不当,导致患者气道梗阻发生。经皮穿刺气管切开置管技术为危重症烧伤急救与成批烧伤的救治提供了便利,中国老年医学学会烧创伤分会组织国内部分专家,就烧伤患者气管切开置管的指征、时机、方法、拔管及注意事项进行讨论,撰写了《烧伤患者气管切开置管全国专家共识(2018版)》,为临床治疗提供参考标准。     

99mTc-MIBI显像在甲状腺癌中的临床应用

目的:介绍99mTc-MIBI显像在甲状腺癌中的临床应用。方法:根据国内外对于99mTc-MIBI显像在甲状腺癌中的研究和应用进行分析和总结。结果:99mTc-MIBI显像在甲状腺癌的早期诊断、检测复发或转移和评价治疗后效果等多方面均有重要价值。结论:99mTc-MIBI显像可以作为一种有效的检查手段应用于甲状腺癌。