流行性出血热并发视网膜色素上皮炎一例

流行性出血热并发视网膜色素上皮炎一例孙水林（第二附属医院传染科南昌３３０００６）１临床资料患者，男，４４岁。１９８８年１月１２月，因发热，头痛，腰痛５ｄ，眼球结膜水肿，口腔粘膜及双腋下皮肤见比较多针尖样出血点，查尿蛋白（），血Ｒｔ：ＷＢＣ２．１×１...     

肾综合征出血热相关细胞因子研究进展

肾综合征出血热(hemorrhagic fever with renal syndrome,HFRS)是汉坦病毒感染引起的以发热、休克、充血、出血和急性肾功能衰竭为主要表现的一种自然疫源性疾病,其基本病变为全身广泛性小血管损害,典型病例临床上有5期病程:发热期、低血压休克期、少尿期、多尿期和恢复期.     

大鼠枯否细胞的分离、鉴定以及LPS刺激诱导TNF-α的表达

目的:分离、培养和鉴定枯否细胞,并探讨LPS刺激对细胞肿瘤坏死因子(tumor necrosis factor-α,TNF-α)表达和分泌的影响.方法:采用在体原位灌注、密度梯度离心和早期细胞换液等方法分离纯化大鼠肝枯否细胞(kupffer cell,KC),并采用墨汁吞噬和ED2染色试验对分离培养的细胞进行鉴定.TNF-α表达和分泌采用RT-PCR和酶联免疫技术(enzyme-linked immunosorbent,ELISA)检测.结果:成功分离和纯化大鼠肝KC,并经墨汁吞噬和ED2染色试验鉴定证实;LPS刺激KC细胞内TNF-α mRNA的表达较非刺激细胞(PBS处理细胞)显著升高(1.10±0.02vs0.09±0.01,P<0.001).另外,LPS刺激较非刺激KC培养上清液TNF-α蛋白的水平也显著升高(487.10pg/mL±5.56pg/mLvs39.41pg/mL±15.30pg/mL,P<0.001).结论:原位灌注和密度梯度离心法能有效分离纯化大鼠KC,LPS刺激可诱导其表达和分泌大量TNF-α.     

《人体形态学》网络教学中护生实践能力及创新精神培养的研究

在护理专业教学改革中，把人体系统解剖学、组织胚胎学和局部解剖学三门课程合并成一门人体形态学，并且把现代教育技术的手段与传统的教学方法有机的结合。为培养高素质现代医学人才，以转变教育教学思想为先导，以现代教育技术作为依托．对人体形态学课程的教学内容、教学方法、教学手段进行整体改革，在实践中逐步构建和完善利用多媒体的理论课教学、实验教学以及网络自主学习三者有机结合的现代人体形态学课程网络教学体系。     

急性肝功能衰竭与基因相关性

急性肝功能衰竭目前没有特异性治疗,病死率极高。随着对基因研究的深入,在急性肝功能衰竭的发生和治疗方面提供了新的思路,此文就急性肝功能衰竭与基因的相关问题进行综述。     

Clinical study of the efficacy of interferon-α therapy for HBeAg-negative chronic hepatitis B patients

Objective To investigate the effect of IFN-α therapy for HBeAg-negative ehronie hepatitis B(CHB). Methods 50 cases of HBeAg-negative CHB patients were selected as treated group, while 52 cases of HBeAg-positive CHB as control group. Both groups received injection of IFN-α at dose of 6 MU every other day for 48 weeks. Levels of alanine aminotransferase, viral markers levels of HBeAg, HBV DNA and the four serum fibrosis markers were analysed before and after treatment and 24 weeks after the course. Results There were 36 cases in treated group and 26 cases in control group who had got obvious therapeutic effects at the end of 24 weeks after treatment. And the rates of efficacy were 72% and 50% separately. The rate of treated group was higher than that of control group(X2 = 5.43, P <0.05). The four serum fibrosis markers of the both groups were clearly dropped after treatment (t = 2.365, P < 0.05). Conclusions The theraputie effects of IFN-α at dose of 6 MU for HBeAg-negative CHB is prior to HBeAg-positive CHB. And IFN-α also have an evident funtion on preventing or delaying hepatic fibrosis in patients with CHB.     

Clinical study of the efficacy of interferon-α therapy for HBeAg-negative chronic hepatitis B patients

Objective To investigate the effect of IFN-α therapy for HBeAg-negative ehronie hepatitis B(CHB). Methods 50 cases of HBeAg-negative CHB patients were selected as treated group, while 52 cases of HBeAg-positive CHB as control group. Both groups received injection of IFN-α at dose of 6 MU every other day for 48 weeks. Levels of alanine aminotransferase, viral markers levels of HBeAg, HBV DNA and the four serum fibrosis markers were analysed before and after treatment and 24 weeks after the course. Results There were 36 cases in treated group and 26 cases in control group who had got obvious therapeutic effects at the end of 24 weeks after treatment. And the rates of efficacy were 72% and 50% separately. The rate of treated group was higher than that of control group(X2 = 5.43, P <0.05). The four serum fibrosis markers of the both groups were clearly dropped after treatment (t = 2.365, P < 0.05). Conclusions The theraputie effects of IFN-α at dose of 6 MU for HBeAg-negative CHB is prior to HBeAg-positive CHB. And IFN-α also have an evident funtion on preventing or delaying hepatic fibrosis in patients with CHB.     

甲状腺区辨认喉返神经的解剖数据

本文在100侧(50具)成人尸体上,从临床应用的角度,调查了喉返神经在甲状腺区的毗邻关系,提出了在甲状腺手术中辨认喉这神经的解剖数据和临床标志,为保护喉返神经提供了可靠的解剖数值依据。     

以颊黏膜多发玫瑰疹为特征的伤寒一例

患者男,14岁.因持续发热1周入院.7 d前无明显诱因出现低热,37.8℃,自服感冒药无效,体温逐日升高达39.6℃,伴畏寒、食欲明显减退、腹胀、便秘,无寒战,无汗.体格检查:体温39.8℃,脉搏92次/min,血压110/70 mmHg(1 mm Hg=0.133 kPa).