Axonal swellings and degeneration in mice lacking the major proteolipid of myelin

Glial cells produce myelin and contribute to axonal morphology in the nervous system. Two myelin membrane proteolipids, PLP and DM20, were shown to be essential for the integrity of myelinated axons. In the absence of PLP-DM20, mice assembled compact myelin sheaths but subsequently developed widespread axonal swellings and degeneration, associated predominantly with small-caliber nerve fibers. Similar swellings were absent in dysmyelinated shiverer mice, which lack myelin basic protein (MBP), but recurred in MBP*PLP double mutants. Thus, fiber degeneration, which was probably secondary to impaired axonal transport, could indicate that myelinated axons require local oligodendroglial support.     

Treatment of human head lice infestations in a single application with a new galenic lotion

To evaluate the efficiency and safety of a new galenic meta‐emulsion for the treatment of human head lice (Pediculus capitis) in a single application. A controlled clinical study was conducted comparing lice infestation and nit hatching observed before and after a treatment in a single application. Eighty‐two of the 87 children included completed the study. An infestation control was performed on 36 and 46 children, 8 and 24 h respectively after application. Five days later, a meticulous hair examination was carried out to check that the lice infestation was completely cured. After a single application of the lotion being tested, an examination of the scalp with a head lice detection comb, as well as an examination of the rinsing water and the towel used for drying after washing, showed that out of the total 1285 lice, there were no live lice. The percentage of nits hatching before treatment was close to 70%. In comparison, after an 8‐h treatment (t₊₈), the percentage of nits hatching was 2.1%, with only 0.35% of living nymphs. After a 24‐h treatment (t₊₂₄), 1.9% hatched with 0.38% living nymphs. Nymphs were revealed to be non‐viable. After 5 days (t₊₁₂₀), no living adult or immature lice were found on the subjects tested. Moreover, observation of tolerance levels to this treatment at days 1, 5 and 12 showed no side effects. The specific galenic lotion completely cured head lice infestation in the population studied in a single application. The lotion, a patented meta‐emulsion, has a mechanical action that asphyxiates lice and nits. Considering the advantages of the single application, the possibility of complete concomitant therapeutics for a whole school population within only 1 day and the high level of tolerance to this treatment, this approach seems simple and promising.     

Hypnotizability, preference for an imagic cognitive style, and memory creation in hypnosis.

We sought to extend the finding (J.-R. Laurence and C. Perry, 1983) that it is possible through hypnosis to create a pseudomemory of a specific event. Subjects were compared on their responses to a hypnotic suggestion. We also examined the relation between nonhypnotic measures (Preference for an Imagic Cognitive Style [PICS] test and the Tellegen Absorption Scale [TAS]) and response to the suggestion. Highly (45.45%) and moderately-highly (46.15%) susceptible subjects believed that the pseudomemory was veridical, but none of the low susceptible subjects did so. Together, hypnotic susceptibility, the PICS, and their interaction more strongly predicted pseudomemory creation than any of these main effects alone. Performance on the TAS was also related to the occurrence of pseudomemory, but this relation was redundant with performance on other measures. These results point to the complexity of the variables involved in pseudomemory creation during hypnosis. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Biogenic amines in vacuum-packaged and carbon dioxide-controlled atmosphere-packaged fresh pork stored at -1.50 degrees C

Biogenic amines are formed in foods as a result of amino acid decarboxylation catalyzed by bacterial enzymes. When consumed in sufficient quantities, these compounds will cause headache, hypertension, fever, and heart failure. Technologies such as vacuum packaging and carbon dioxide-modified atmosphere packaging (CO2-MAP), when combined with low-temperature storage (-1.5 degrees C), allow fresh pork to have a storage life long enough for export to overseas markets. During low-temperature storage of pork in these packaging systems, the lactic acid bacteria (LAB), which possess the enzymes for biogenic amine formation, dominate the microflora. The objectives of this study were to determine the quantities of biogenic amines in packaged fresh pork, to monitor LAB growth, and to determine the storage life by sensory evaluation. Vacuum-packaged and CO2-MAP pork were stored at -1.5+/-0.5 degrees C for 9 and 13 weeks, respectively. Phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine concentrations were determined weekly by high-performance liquid chromatography and capillary gel electrophoresis. LAB and carnobacteria were enumerated weekly. Samples were evaluated for odor and appearance. The CO2-MAP was successful in delaying bacterial growth and the development of unacceptable off-odors compared with the vacuum packaging. The storage lives of the vacuum-packaged and CO2-MAP pork were 5 and 13 weeks, respectively. High-performance liquid chromatography was the superior method for biogenic amine quantification. Tyramine and phenylethylamine in pork of both packaging treatments approached levels considered to be potentially toxic. Given Canada's increasing role in the export of fresh meat to foreign markets, it is recommended that the formation of biogenic amines in vacuum-packaged and CO2-MAP pork be further investigated.     

Occurrence and characterization of Salmonella from chicken nuggets, strips, and pelleted broiler feed

Raw, frozen chicken nuggets and strips have been identified as a significant risk factor in contracting foodborne salmonellosis. Cases of salmonellosis as a result of consuming partly cooked chicken nuggets may be due in part to Salmonella strains originating in broiler feed. This study was undertaken to determine the occurrence and characterize the strains of Salmonella contaminating chicken nuggets, strips, and pelleted feeds, in an attempt to demonstrate whether the same Salmonella strains present in broiler feed could be isolated from raw, frozen chicken nuggets and strips available for human consumption. Salmonellae were recovered using the Health Canada MFHPB-20 method for the isolation and identification of Salmonella from foods. Strains were characterized by serotyping, phage typing, antimicrobial resistance typing (R-typing), and by pulsed-field gel electrophoresis (PFGE). Salmonellae were isolated from 25-g samples in 27% (n=92) of nugget and strip samples, 95% (n=20) of chicken nugget meat samples, and from 9% (n=111) of pelleted feed samples. Salmonella Heidelberg, Salmonella Enteritidis, and Salmonella Orion were the most commonly isolated serovars from chicken nuggets and strips, nugget and strip meat, and pelleted broiler feeds, respectively. Salmonella Enteritidis phage type (PT) 13a with PFGE pattern SENXAI.0006 and R-type sensitive as well as Salmonella Enteritidis PT13a with PFGE pattern SENXAI.0068 and R-type sensitive were isolated from pelleted feed, and chicken nugget and strip meat in two separate instances. Data showed that Salmonella strains isolated from broiler feed were indistinguishable from strains isolated from packaged raw, frozen chicken nuggets and strips. However, results did not rule out the possibility that breeding stock or contamination during processing may have contributed to chicken meat contamination by Salmonella.     

Satellite-based mapping of Canadian boreal forest fires: Evaluation and comparison of algorithms

This paper evaluates annual fire maps that were produced from NOAA-14/AVHRR imagery using an algorithm described in a companion paper (Li et al., International Journal of Remote Sensing, 21, 3057-3069, 2000 (this issue)). Burned area masks covering the Canadian boreal forest were created by compositing the daily maps of fire hot spots over the summer and by examining Normalized Difference Vegetation Index (NDVI) changes after burning. Both masks were compared with fire polygons derived by Canadian fire agencies through aerial surveillance. It was found that the majority of fire events were captured by the satellite-based techniques, but burnt area was generally underestimated. The burn boundary formed by the fire pixels detected by satellite were in good agreement with the polygons boundaries within which, however, there were some fires missed by the satellite. The presence of clouds and low sampling frequency of satellite observation are the two major causes for the underestimation. While this problem is alleviated by taking advantage of NDVI changes, a simple combination of a hot spot technique with a NDVI method is not an ideal solution due to the introduction of new sources of uncertainty. In addition, the performance of the algorithm used in the International Geosphere-Biosphere Programme (IGBP) Data and Information System (IGBPDIS) for global fire detection was evaluated by comparing its results with ours and with the fire agency reports. It was found that the IGBP-DIS algorithm is capable of detecting the majority of fires over the boreal forest, but also includes many false fires over old burned scars created by fires taking place in previous years. A step-by-step comparison between the two algorithms revealed the causes of the problem and recommendations are made to rectify them.     

The spatial dependency of shoulder muscular demands during upward and downward exertions

Lifting and lowering are common occupational tasks contributing to shoulder injury risk. Quantifying task interaction with physical demand can precipitate better workstation designs. Nineteen university-aged males performed one-handed, submaximal upward/downward manual force exertions at 70 hand locations; unilateral electromyography (EMG) of 14 muscles was recorded. EMG across planes was evaluated with ANOVA. Predictive equations for muscle activity throughout the reach envelope were developed with stepwise regression. Total muscle activity (sum of individual muscle activity) was most sensitive to vertical hand location for upward exertions, where activation at superior locations was 192% of values for inferior locations. For upward exertions, activation differences for hand location occurred along all anatomical axes, and along anterior/posterior and superior/inferior axes for downward exertions. Predictive equations were non-linear, reflecting complex muscular demand with three-dimensional hand location. This work details foundational exposure data for lifting/lowering exertions. Results are applicable to workstation design to minimise occupational shoulder muscular demands.

Practitioner Summary: Lifting and lowering in the workplace contribute to shoulder injury risk. Shoulder muscle activity magnitudes revealed a dependence on three-dimensional hand location in the reach envelope for a defined hand force. This information can inform evidence-based workstation designs that reduce shoulder muscular demands for numerous materials handling scenarios.