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1.
Caroline Van De Wauwer Arne P. Neyrinck Nele Geudens Filip R. Rega Geert M. Verleden Erik Verbeken Toni E. Lerut Dirk E.M. Van Raemdonck 《European journal of cardio-thoracic surgery》2007,31(6):1125-1133
Objective: The use of non-heart-beating donors (NHBD) has been propagated as an alternative to overcome the scarcity of pulmonary grafts. Formation of microthrombi after circulatory arrest, however, is a major concern for the development of reperfusion injury. We looked at the effect and the best route of pulmonary flush following topical cooling in NHBD. Methods: Non-heparinized pigs were sacrificed by ventricular fibrillation and divided into three groups (n = 6 per group). After 1 h of in situ warm ischaemia and 2.5 h of topical cooling, lungs in group I were retrieved unflushed (NF). In group II, lungs were explanted following an anterograde flush (AF) through the pulmonary artery with 50 ml/kg Perfadex® (6 °C). Finally, in group III, lungs were retrieved after an identical but retrograde flush (RF) via the left atrium. Flush effluent was sampled at intervals to measure haemoglobin concentration. Performance of the left lung was assessed during 60 min in our ex vivo reperfusion model. Wet-to-dry weight ratio (W/D) of both lungs was calculated as an index of pulmonary oedema. IL-1ß and TNF- protein levels in bronchial lavage fluid from both lungs were compared between groups. Results: Haemoglobin concentration (g/dl) was higher in the first effluent in RF versus AF (3.4 ± 1.1 vs 0.6 ± 0.1; p < 0.05). Pulmonary vascular resistance (dynes × s × cm−5) was 975 ± 85 RF versus 1567 ± 98 AF and 1576 ± 88 NF at 60 min of reperfusion (p < 0.001). Oxygenation (mmHg) and compliance (ml/cmH2O) were higher (491 ± 44 vs 472 ± 61 and 430 ± 33 NS, 22 ± 3 vs 19 ± 3 and 14 ± 1 NS, respectively) and plateau airway pressure (cmH2O) was lower (11 ± 1 vs 13 ± 1 and 13 ± 1 NS) after RF versus AF and NF, respectively. No differences in cytokine levels or in W/D ratios were observed between groups after reperfusion. Histology demonstrated microthrombi more often present after AF and NF compared to RF. Conclusion: Retrograde flush of the lung following topical cooling in the NHBD results in a better washout of residual blood and microthrombi and subsequent reduced pulmonary vascular resistance upon reperfusion. 相似文献
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Intimal hyperplasia after long-term venous catheterization 总被引:1,自引:0,他引:1
Xiang DZ Verbeken EK Van Lommel AT Stas M De Wever I 《European surgical research. Europ?ische chirurgische Forschung. Recherches chirurgicales européennes》2000,32(4):236-245
OBJECTIVE: Intimal hyperplasia is a well-known consequence of arterial injury and arterialization in vein grafts. However, the subacute and chronic vein wall changes which occur after catheterization have not been well studied. In this animal study, intimal hyperplasia in the vein wall after catheterization was examined. METHODS: A silicon catheter was placed in the anterior caval vein of 54 rats. After in situ fixation at scheduled intervals (1 day to 6 months), the pathologic changes in the vein wall were studied on semi-serial histology sections by means of light microscopy. RESULTS: Three forms of intimal hyperplasia could be observed: plaque-like, papillary-like and incorporation of the mural part of the sleeve into the underlying vein wall. Although the appearance of each was different, their composition was identical. All were mainly composed of alpha-actin-positive cells and collagen localized above the internal elastin layer, and covered by endothelium if facing the lumen. The plaque-like and papillary-like forms were mainly localized in the anterior vena cava, while sleeve incorporation mainly occurred in the jugular vein. Plaque-like and papillary-like intimal hyperplasia could be seen together on the same slide, but these two forms were never seen together with sleeve incorporation. CONCLUSION: Intimal hyperplasia occurs after venous catheterization and is probably caused by chronic injury to the vein wall due to knocking and rubbing movements of the catheter against the wall. 相似文献
4.
Undine Rulle Zoi Tsourti Ruben Casanova Karl-Friedrich Deml Eric Verbeken Erik Thunnissen Arne Warth Richard Cheney Aleksandra Sejda Ernst Jan Speel Line Bille Madsen Daisuke Nonaka Atilio Navarro Irene Sansano Antonio Marchetti Stephen P. Finn Kim Monkhorst Keith M. Kerr Alex Soltermann 《Journal of thoracic oncology》2018,13(12):1851-1863
Introduction
Phosphatase and tensin homolog (PTEN) loss is frequently observed in NSCLC and associated with both phosphoinositide 3-kinase activation and tumoral immunosuppression. PTEN immunohistochemistry is a valuable readout, but lacks standardized staining protocol and cutoff value.Methods
After an external quality assessment using SP218, 138G6 and 6H2.1 anti-PTEN antibodies, scored on webbook and tissue microarray, the European Thoracic Oncology Platform cohort samples (n = 2245 NSCLC patients, 8980 tissue microarray cores) were stained with SP218. All cores were H-scored by pathologists and by computerized pixel-based intensity measurements calibrated by pathologists.Results
All three antibodies differentiated six PTEN+ versus six PTEN- cases on external quality assessment. For 138G6 and SP218, high sensitivity and specificity was found for all H-score threshold values including prospectively defined 0, calculated 8 (pathologists), and calculated 5 (computer). High concordance among pathologists in setting computer-based intensities and between pathologists and computer in H-scoring was observed. Because of over-integration of the human eye, pixel-based computer H-scores were overall 54% lower. For all cutoff values, PTEN- was associated with smoking history, squamous cell histology, and higher tumor stage (p < 0.001). In adenocarcinomas, PTEN- was associated with poor survival.Conclusion
Calibration of immunoreactivity intensities by pathologists following computerized H-score measurements has the potential to improve reproducibility and homogeneity of biomarker detection regarding epitope validation in multicenter studies. 相似文献5.
Dual-phase low-alloy steels combine a soft ferrite phase with a hard martensite phase to create desirable properties in terms of strength and ductility. Nickel additions to dual-phase low-alloy steels can increase the yield strength further and lower the transformation temperatures, allowing for microstructure refining. Determining the correct intercritical annealing temperature as a function of nickel content is paramount, as it defines the microstructure ratio between ferrite and martensite. Likewise, quantifying the influence of nickel on the intercritical temperature and its synergistic effect with the microstructure ratio on mechanical properties is vital to designing dual-phase steels suitable for corrosive oil and gas services as well as hydrogen transport and storage applications. In this work, we used a microstructural design to develop intercritical annealing heat treatments to obtain dual-phase ferritic–martensitic low-alloy steels. The intercritical annealing and tempering temperatures and times were targeted to achieve three different martensite volume fractions as a function of nickel content, with a nominal content varying between 0, 1, and 3-wt% Ni. Mechanical properties were characterized using tensile testing and microhardness measurements. Additionally, the microstructure was studied using scanning electron microscopy coupled with electron backscatter diffraction analysis. Tensile strength increased with increasing martensite ratio and nickel content, with a further grain refinement effect found in the 3-wt% Ni steel. The optimal heat treatment parameters for oil and gas and hydrogen transport applications are discussed. 相似文献
6.
Van Hove JL Jaeken J Proesmans M Boeck KD Minner K Matthijs G Verbeken E Demunter A Boogaerts M 《American journal of medical genetics. Part A》2005,(2):152-158
Two siblings from a consanguineous family presented with a poikiloderma of limbs and face, plantar keratoderma, and toenail pachyonychia. Neutropenia and neutrophil dysfunction with impairment of the respiratory burst and bacterial killing resulted in frequent respiratory tract infections. A bronchocentric granulomatous pneumonia was a fatal complication. The clinical presentation is consistent with Clericuzio type poikiloderma with neutropenia. Literature review identified several additional probable patients. Genetic linkage analysis excluded the locus of the RECQL4 gene, mutations in which have been described in some patients with the Rothmund-Thomson poikiloderma syndrome. This report confirms the clinical and genetic identity of the Clericuzio type of poikiloderma with neutropenia syndrome. 相似文献
7.
E K Verbeken M Cauberghs I Mertens J Clement J M Lauweryns K P Van de Woestijne 《Chest》1992,101(3):793-799
As part of a study of the structural-functional correlations of excised human lungs obtained at autopsy, the parenchyma and peripheral airways were examined by means of morphometric techniques. Among the 30 lungs characterized by the absence of fibrosis, ten differed from the normal and emphysematous lungs by a homogeneous dilatation of the airspaces, in excess of the dimensions predicted on the basis of age. Study of the standard deviations of the mean linear intercepts showed that the airspace dilatation was more regular than in emphysematous lungs; in addition, there was no clear-cut destruction, as estimated from the number of alveolar attachments. These lungs were characterized in addition by an increased thickening of alveolar septa, without inflammation or fibrosis, normal size of the diameter, and reduced density of the membranous bronchioles. Since these lungs were from people older than 60 years, it is assumed that they represent cases of exaggerated airspace enlargement of the aging lung, differing from emphysema by the absence of destruction of alveolar walls. The term "senile lung" is proposed or this condition. 相似文献
8.
Meschi Nastaran Vanhoenacker Anke Strijbos Olaf Camargo dos Santos Bernardo Rubbers Eléonore Peeters Valerie Curvers Frederik Van Mierlo Maarten Geukens Arne Fieuws Steffen Verbeken Eric Lambrechts Paul 《Clinical oral investigations》2020,24(12):4439-4453
Clinical Oral Investigations - The aim of this study was to assess in a multi-modular manner the bone healing 1 year post root-end surgery (RES) with leukocyte- and platelet-rich fibrin... 相似文献
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10.
Balkema C Meersseman W Hermans G Stroobants S Verhoef G Verbeken E Demaerel P Blockmans D 《Acta clinica Belgica》2008,63(3):185-189
Intravascular lymphoma (IVL) is a rare subtype of extranodal diffuse large B-cell lymphoma. It is characterized by proliferation of neoplastic Lymphoid cells almost exclusively within the lumina of small blood vessels. It can affect virtually every organ system. Due to its rarity and its diverse and heterogeneous clinical presentation, diagnosis is difficult and often made post-mortem. When diagnosed early, it is, however, potentially treatable. We present a young woman with longstanding constitutional symptoms, positive antinuclear antibody, elevated LDH levels and rapidly progressive encephalopathy. FDG-PET scan showed intense uptake in the renal cortex, which prompted us to perform a kidney biopsy which was compatible with IVL. The value of PET in establishing the diagnosis of this rare disease will be discussed. 相似文献