Hindshaker, a novel myelin mutant showing hypomyelination preferentially affecting the spinal cord

Animals with spontaneous mutations affecting myelin formation have provided useful information about the genetic and cellular mechanisms regulating normal and abnormal myelination. In this paper we describe a novel murine mutation termed hindshaker (hsh), which is inherited in an autosomal recessive manner. Affected mice are characterised by a variable tremor of the hind end which commences at about 2 weeks of age and largely disappears in animals older than 6 weeks. There is hypomyelination affecting predominantly the spinal cord, although the optic nerves and brain are involved to a much lesser degree. The defect of thinly myelinated and naked axons is maximal at 20 days of age and largely resolves with time so that in the adult most axons are myelinated. The myelin structure appears normal and immunostains for the major proteins. Although the distribution of oligodendrocytes in the spinal cord is similar to normal during the period of hypomyelination, there are fewer mature cells. The hsh mutation appears to delay the maturation of oligodendrocytes, particularly in the spinal cord. Additionally, there is a considerable variation in phenotypic expression and in penetrance when the mutation is expressed on different genetic backgrounds, suggesting the hsh locus is subject to the influence of modifying gene(s). Identification of the hsh gene should identify a factor important in the development of oligodendrocytes, particularly those in the spinal cord.     

International coordination of operational instruments on the NASAand ESA space station polar orbiting platforms

The roles played by the National Oceanic and Atmospheric Administration (NOAA) and the international community in coordinating plans to fly operational instruments on NOAA polar orbiting satellites are discussed, as is how this coordination has assisted in the planning for the flight of similar instruments on the proposed NASA and ESA polar orbiting platforms in the mid-to-late-1990s. Details on the operational sensor complements on the polar platforms are provided     

Anionic synthesis of poly(epichlorohydrin-g-styrene) and chemical modification of polyepichlorohydrin

Poly(epichlorohydrin-g-styrene) copolymers, P(ECH-g-S)s, have been prepared in solution by two different methods of coupling polyepichlorohydrin, PECH, with polystyrene, PS, containing a functional end group. In the first method, polystyryl carboxylic acid, PSCOOH, prepared from living polystyrene, was converted to the corresponding potassium salt which was coupled with PECH in dimethyl formamide solution usually at 60°C. In the second method, polystyryl potassium, PS^?K⁺, in tetrahydrofuran solution was end-capped with propylene sulphide to form PSCH₂CH(CH₃)S^?K⁺ which was coupled directly with PECH in tetrahydrofuran at 40°C. Grafting by the first reaction was relatively slow but occurred without significant adverse side reactions. The grafting by the second reaction was relatively fast, but side reactions occurred resulting in a cleavage of the graft copolymer. The graft copolymers were purified by fractional precipitation and extraction procedures and were characterized by infra-red and ultra-violet spectroscopy, microanalysis, gel permeation chromatography, differential scanning calorimetry, solution viscometry, dynamic mechanical testing, and electron microscopy. Solution properties of the graft copolymers in benzene-carbon tetrachloride and toluene-cyclohexane mixtures were studied. There was evidence of micelle formation in dilute solutions of the mixed solvents and microphase separation of components in the solid state. Nucleophilic substitution of chlorine atoms in PECH by phenyl acetate (C₆H₅ CH₂COO-) and thiophenoxide (C₆H₅S-) groups was performed.     

Retarding effects of DNA on the autoxidation of liposomal suspensions

Deoxyribonucleic acid (DNA) is associated with the cell membrane of prokaryotes and the inner nuclear membrane of eukaryotes. The unsaturated fatty acids of phospholipids, which constitute the bilaminar structure of membranes, undergo autoxidation in the presence of O₂. Calf thymus DNA was incubated with methyl archidonate-enriched phosphatidyl choline liposomes in order to study the effect of DNA upon the oxidation of phospholipids while present in their natural in vivo bilayer configuration. DNA retarded the rate of lipid oxidation and the TBA test, but it did not alter the induction period. These results suggest that DNA is scavenging free radicals produced within the phospholipid bilayer.     

Optics clustered to output unique solutions: a multi-laser facility for combined single molecule and ensemble microscopy

Optics clustered to output unique solutions (OCTOPUS) is a microscopy platform that combines single molecule and ensemble imaging methodologies. A novel aspect of OCTOPUS is its laser excitation system, which consists of a central core of interlocked continuous wave and pulsed laser sources, launched into optical fibres and linked via laser combiners. Fibres are plugged into wall-mounted patch panels that reach microscopy end-stations in adjacent rooms. This allows multiple tailor-made combinations of laser colours and time characteristics to be shared by different end-stations minimising the need for laser duplications. This setup brings significant benefits in terms of cost effectiveness, ease of operation, and user safety. The modular nature of OCTOPUS also facilitates the addition of new techniques as required, allowing the use of existing lasers in new microscopes while retaining the ability to run the established parts of the facility. To date, techniques interlinked are multi-photon/multicolour confocal fluorescence lifetime imaging for several modalities of fluorescence resonance energy transfer (FRET) and time-resolved anisotropy, total internal reflection fluorescence, single molecule imaging of single pair FRET, single molecule fluorescence polarisation, particle tracking, and optical tweezers. Here, we use a well-studied system, the epidermal growth factor receptor network, to illustrate how OCTOPUS can aid in the investigation of complex biological phenomena.     

Focus groups reveal perils and promises of managed care for nurse practitioners

Decades of practice and research suggest that nurse practitioners (NPs) provide cost-effective and high-quality care. Managed care's emphasis on prevention and cost savings led some policy makers to view NPs as a way to meet the need for primary care providers. However, access to and utilization of NPs has increasingly been controlled by managed care organizations (MCOs) through their selection of providers for primary care panels. This study employed qualitative methodology to examine NPs' experiences with MCOs. Three focus groups, comprising 27 NPs in New York and Connecticut, revealed NPs' mixed reactions to managed care and a range of sentiments regarding NPs' efforts to be listed as primary care providers. The results reflected NPs' concerns about their perceived "invisibility," as well as their sense of "invincibility" in the ways in which NPs are responding to the barriers posed by MCOs. They identified barriers to, as well as ways to facilitate, being listed by MCOs, and described the importance of NPs working individually and collectively in negotiating with MCOs.     

Relative importance,specific investment and ownership in interorganizational systems

Implementation and maintenance of interorganizational systems (IOS) require investments by all the participating firms. Compared with intraorganizational systems, however, there are additional uncertainties and risks. This is because the benefits of IOS investment depend not only on a firm’s own decisions, but also on those of its business partners. Without appropriate levels of investment by all the firms participating in an IOS, they cannot reap the full benefits. Drawing upon the literature in institutional economics, we examine IOS ownership as a means to induce value-maximizing noncontractible investments. We model the impact of two factors derived from the theory of incomplete contracts and transaction cost economics: relative importance of investments and specificity of investments. We apply the model to a vendor-managed inventory system (VMI) in a supply chain setting. We show that when the specificity of investments is high, this is a more critical determinant of optimal ownership structure than the relative importance of investments. As technologies used in IOS become increasingly redeployable and reusable, and less specific, the relative importance of investments becomes a dominant factor. We also show that the bargaining mechanism—or the agreed upon approach to splitting the incremental payoffs—that is used affects the relationship between these factors in determining the optimal ownership structure of an IOS.

Surface waves on a homogeneously fluidized bed

Summary This paper examines the small-amplitude free-surface motions on a homogeneously fluidized bed for which the density ratio % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaeqyWdi3aaS% baaSqaaiaadAgaaeqaaOGaai4laiabeg8aYnaaBaaaleaacaWGZbaa% beaakiablQMi9iaaigdaaaa!3E84!\[\rho _f /\rho _s \ll 1\], where _f is the fluid density and _s is the solid density. It is shown that to leading order in % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaeqyWdi3aaS% baaSqaaiaadAgaaeqaaOGaai4laiabeg8aYnaaBaaaleaacaWGZbaa% beaaaaa!3C65!\[\rho _f /\rho _s \], the surface motions are independent of the dynamics of the bed varying only with the dimensions of the bed and the conditions at uniform fluidization. Also, at leading order, it is found that provided the fluidization remains homogeneous there is a direct analogy between waves on the free surface of a fluidized bed and waves on the free surface of an inviscid liquid (when surface tension is neglected).     

The evolution of a two-dimensional small-amplitude voidage disturbance in a uniformly fluidized bed

Summary The evolution of a small-amplitude localized voidage disturbance through a two-dimensional fluidized bed is considered. A solution of the full initial-value problem reveals that the stability condition for the uniform state is found to be in agreement with that given by Needham and Merkin [1] for narrow beds. Further, it is shown that for a given flow rate for which the uniform state is unstable, more and more cross-channel modes become instable with increasing bed width, in accordance with the observations of Didwania and Homsy [3]. Finally a solution is obtained forF 1, whereF is the Froude number. The resulting Fourier series is summed numerically, which enables the evolution of the initial disturbance to be followed by plotting contours of constant voidage with increasing time. This shows that in narrow beds the motion is primarily one-dimensional, confirming the supposition of [1], with cross-stream variations becoming more dominant as the bed width increases.