Biotransformation of 2,4,6-trinitrotoluene (TNT) by a Methanococcus sp. (strain B) isolated from a lake sediment

The newly developed antihypertensive drugs, the long-acting beta-blocker propranolol and the sustained release calcium antagonist verapamil, are compared in their antihypertensive, platelet function, rheological properties and metabolic effects. The trial was a double-blind, randomised, placebo-controlled cross-over study. Thirty patients with mild to moderate hypertension received propranolol (40-120 mg) or verapamil (80-200 mg) once daily in two separate ten week courses. After ten weeks treatment both drugs had significantly reduced both SBP and DBP. Beta-thromboglobulin (beta-TG) concentration, reflecting the status of platelet activation in vivo, was significantly decreased after propranolol (129.6 +/- 13.5 vs. 77.9 +/- 8.6 ng/ml) and verapamil (129.6 +/- 13.5 vs. 90.7 +/- 10.1 ng/ml) treatments while platelet aggregation induced by ADP, collagen, arachidonic acid or adrenaline and the production of thromboxane B2 (TXB2), 6-ketoprostaglandin F1 alpha (6-keto-PGF1 alpha) and platelet cyclic 3'-5' adenosine monophosphate (C-AMP) concentration were not affected. Significant alterations in rheological parameters such as plasma and whole blood viscosity, fibrinogen level and red cell deformability were not found. Higher cholesterol and low-density lipoprotein-cholesterol (LDL-C) levels were observed after propranolol treatment but not in verapamil treatment. Side-effects were mild, tolerated and no patient had to be withdrawn from the study. In conclusion, propranolol and verapamil are generally effective antihypertensive as well as rheologically safe drugs. Compared with the metabolic effect on serum lipid, verapamil may be a better choice. Both drugs possess the tendency to inhibit platelet properties which is desirable in hypertension treatment.     

Process optimization for enhanced production of diphtheria toxin by submerged cultivation

When stationary culture was replaced by submerged cultivation in a fermentor, a significant increase in the yield of diphtheria toxin in a short cultivation time (less than 48 h as against 7-8 d) was noted. It was found that under optimal conditions of temperature, vortex mixing and surface aeration, an alkaline pH favoured toxin release. Furthermore, to enhance the production volume, two-and three-step semicontinuous batch cultivations were carried out. The toxin produced was of good titre with an adequate antigenic purity. Under optimal conditions, the variation in the Limes of flocculation (Lf titre) was likely due to the quality of the production medium, which in turn depended on the quality of the raw materials used. The process was also optimized in a pilot-scale fermentor.     

Creep Behavior of Hydrogenated Zirconium Alloys

Zirconium (Zr) alloys are the primary structural materials of most water reactors. Creep is considered to be one of the important degradation mechanisms of Zr alloys during reactor operating and repository conditions. Zr alloys pick up hydrogen (H₂) during their service from the coolant water. Hydrogen can be present in solid solution or precipitated hydride form in Zr alloys depending upon the temperature and concentration. This study reviews the effect of hydrogen on creep behavior of Zr alloys used in the water reactors.     

Development and characterisation of structurally reforming engineered flat-rice xerogel for hot water cooking

Shape transforming foods are an emerging area of interest that could be customised according to consumer needs with the desirable structure and reduced package space. The current work aims to investigate the shape transformation behaviour of a 2D xerogel developed from rice hydrogel at the concentration of 4% and 90 °C. This flat xerogel is coated with ethyl cellulose as constraint material in the form of linear equally spaced strips. Immersing the xerogels in hot water over a temperature of 70 °C, resulted in a 3D transformation of the discs in 12 s (Video  S1 ). The transformation is achieved owing to xerogel's increased swelling power (0.27–0.78) due to the anisotropic nature. This is confirmed by the SEM images of top (smooth) and bottom (rough) xerogel. In order to characterise the process of shape transformation bending analysis was done wherein a significant difference in bending angle (35.86–63.10°), height (24.73–50.26 mm), curvature (0.57–2.38 μ mm⁻¹) and end-to-end distance (118.23–15.11 mm) from 2 to 12 s. The formed 3D shape was similar to the commercial tubular (penne) pasta. This study opens up the utilisation of less concentrated rice xerogel in shape transformation for designing engineered food.     

Characterisation and recovery of sodium chloride from salt-laden solid waste generated from leather industry

In this present investigation, physical and chemical characteristics of salt-laden solid waste (SLSW) generated from leather industry during soaking process of raw skins/hides and reverse osmosis reject stream were determined. The disposal of SLSW onto landfill was banned due to the possibility of ground water contamination by leachate, and its treatment process becomes tedious and cost intensive. The detailed characterisation of SLSW was evaluated for the development of effective methods for the treatment and/or disposal of SLSW. In the present study, sodium chloride was recovered to reduce the impact of the disposal of SLSW. The recovery of sodium chloride was achieved by selective precipitation using hydrogen chloride gas as a precipitating agent. The optimum conditions for the precipitation of sodium chloride were concentration of SLSW, 60 % (w/w); HCl gas purging, time 3 min (for a batch volume of 100 mL); pH, 8.0; and temperature, 30–40 °C. The scanning electron microscopy images of SLSW and the recovered sodium chloride were captured to observe the surface morphology. The recovered salt showed purity equivalent to that of standard reference salt. The recovered salt can be reused within leather sector or to the other process industries that meet their specification.     

Application of grey fuzzy logic for the optimization of drilling parameters for CFRP composites with multiple performance characteristics

Carbon Fibre Reinforced Plastic (CFRP) composite materials have potential applications in various domains. In machining, drilling is essentially required to join different structures. But CFRP drilling poses many problems that decrease the quality of holes. In this paper, Taguchi’s L₂₇ orthogonal array is used to perform drilling of CFRP composite plates. To improve the quality of the holes drilled, the optimal combination of drilling parameters is chosen using grey relational analysis. Grey fuzzy optimization of drilling parameters is based on five different output performance characteristics, namely, thrust force, torque, entry delamination, exit delamination and eccentricity of the holes. Analysis of variance (ANOVA) is used to find the percentage contribution of the drilling parameters and found that feed rate is the most influential factor in drilling of CFRP composites.     

Ruthenium Pincer‐Catalyzed Acylation of Alcohols Using Esters with Liberation of Hydrogen under Neutral Conditions

Acylation of secondary alcohols using non‐activated esters, in particular symmetrical esters (such as ethyl acetate), is achieved under neutral conditions with the liberation of molecular hydrogen. This unprecedented, environmentally benign reaction is homogenously catalyzed by a dearomatized ruthenium pincer PNN complex.     

Anti-SOD1 Nanobodies That Stabilize Misfolded SOD1 Proteins Also Promote Neurite Outgrowth in Mutant SOD1 Human Neurons

ALS-linked mutations induce aberrant conformations within the SOD1 protein that are thought to underlie the pathogenic mechanism of SOD1-mediated ALS. Although clinical trials are underway for gene silencing of SOD1, these approaches reduce both wild-type and mutated forms of SOD1. Here, we sought to develop anti-SOD1 nanobodies with selectivity for mutant and misfolded forms of human SOD1 over wild-type SOD1. Characterization of two anti-SOD1 nanobodies revealed that these biologics stabilize mutant SOD1 in vitro. Further, SOD1 expression levels were enhanced and the physiological subcellular localization of mutant SOD1 was restored upon co-expression of anti-SOD1 nanobodies in immortalized cells. In human motor neurons harboring the SOD1 A4V mutation, anti-SOD1 nanobody expression promoted neurite outgrowth, demonstrating a protective effect of anti-SOD1 nanobodies in otherwise unhealthy cells. In vitro assays revealed that an anti-SOD1 nanobody exhibited selectivity for human mutant SOD1 over endogenous murine SOD1, thus supporting the preclinical utility of anti-SOD1 nanobodies for testing in animal models of ALS. In sum, the anti-SOD1 nanobodies developed and presented herein represent viable biologics for further preclinical testing in human and mouse models of ALS.