Membrane topology and glycosylation of the human multidrug resistance-associated protein

The membrane topology of the human multidrug resistance-associated protein (MRP) was examined by flow cytometry phenotyping, immunoblotting, and limited proteolysis in drug-resistant human and baculovirus-infected insect cells, expressing either the glycosylated or the underglycosylated forms of this protein. Inhibition of N-linked glycosylation in human cells by tunicamycin did not inhibit the transport function or the antibody recognition of MRP, although its apparent molecular mass was reduced from 180 kDa to 150 kDa. Extracellular addition of trypsin or chymotrypsin had no effect either on the function or on the molecular mass of MRP, while in isolated membranes limited proteolysis produced three large membrane-bound fragments. These experiments and the alignment of the MRP sequence with the human cystic fibrosis transmembrane conductance regulator (CFTR) suggest that human MRP, similarly to CFTR, contains a tandem repeat of six transmembrane helices, each followed by a nucleotide binding domain, and that the C-terminal membrane-bound region is glycosylated. However, the N-terminal region of MRP contains an additional membrane-bound, glycosylated area with four or five transmembrane helices, which seems to be a characteristic feature of MRP-like ATP-binding cassette transporters.     

Graft Copolymerization of Acrylamide onto Oil Palm Empty Fruit Bunch (OPEFB) Fiber

Grafting of acrylamide (AAm) onto oil palm empty fruits bunch fiber using hydrogen peroxide as initiator and methyl acrylate as comonomer was investigated. The amount of comonomer needed to make grafting of acrylamide possible was determined. The percentage of poly(acrylamide) and the comonomer in the final graft copolymer was estimated by elemental analysis. Results obtained indicated that methyl acrylate facilitated the incorporation of acrylamide monomer onto OPEFB. The reactivity ratios for both monomers were determined by using Fineman–Ross plot. The effects of reaction temperature and period as well as amount of the initiator, solvent, monomer and comonomer on the percentage of grafting at fixed amount of comonomer (11 mmol) were studied. Maximum percentage of grafting was achieved when the amount of initiator and solvent 3.98×10⁻³ mol and 50 mL respectively. The optimum reaction temperature was 50 ^○C and the reaction period was 90 min. Highest percentage of grafting was 232% when 25.6 mmol of acrylamide was used under these optimum conditions. The presence of functional group in the grafted polymer is characterized by infrared spectroscopy and the surface morphology is observed by scanning electron microscopy. Thermoanalytic investigation on OPEFB and OPEFB-g-PAAM were carried out to evaluate the thermal stability and respective activation energy of the materials.     

Robust face recognition against expressions and partial occlusions

Facial features under variant-expressions and partial occlusions could have degrading effect on overall face recognition performance. As a solution, we suggest that the contribution of these features on final classification should be determined. In order to represent facial features contribution according to their variations, we propose a feature selection process that describes facial features as local independent component analysis(ICA) features. These local features are acquired using locally lateral subspace(LLS) strategy.Then, through linear discriminant analysis(LDA) we investigate the intraclass and interclass representation of each local ICA feature and express each feature s contribution via a weighting process. Using these weights, we define the contribution of each feature at local classifier level. In order to recognize faces under single sample constraint, we implement LLS strategy on locally linear embedding(LLE) along with the proposed feature selection. Additionally, we highlight the efficiency of the implementation of LLS strategy. The overall accuracy achieved by our approach on datasets with different facial expressions and partial occlusions such as AR, JAFFE,FERET and CK+ is 90.70%. We present together in this paper survey results on face recognition performance and physiological feature selection performed by human subjects.     

A cross-layer approach for partition detection at overlay layer for structured P2P in MANETs

Due to limited radio range and mobility of nodes in mobile ad hoc networks (MANETs), the network partitioning and merging could occur frequently. When structured peer-to-peer (P2P) overlays are running over MANETs, then network partition in the physical network can also cause network partition at the overlay layer. Existing approaches for structured P2P overlay over MANETs do not detect network partition at the overlay layer. This paper proposes a cross-layer approach to detect network partition at the overlay layer for structured P2P overlay over MANETs. Simulation results show that the proposed approach is highly effective and efficient in terms of routing overhead, success ratio and false-negative ratio.     

DeepClassRooms: a deep learning based digital twin framework for on-campus class rooms

Neural Computing and Applications - A lot of different methods are being opted for improving the educational standards through monitoring of the classrooms. The developed world uses Smart...     

On the Efficiency of Runs Rules Schemes for Process Monitoring

There are two main types of variations, namely, common and special causes leading to in‐control and out‐of‐control states, respectively. Control charts are popular tools used to differentiate between these two states of a process. Implementation of runs rules schemes with control charts is an attractive approach for process monitoring. This study is designed to describe the methodology of runs rules schemes and discuss their implementation for different types of control charts. We have considered memory‐less charts, namely, , S, and R charts for our study purposes. It is examined that the efficiency gain depends on the number of decision points utilized to implement a given rule. Moreover, superiority of runs rules schemes may vary for different types of location and dispersion charts. An application example using a dataset is also included in the study for practical considerations. Copyright © 2015 John Wiley & Sons, Ltd.     

Experiencing flow with instant messaging and its facilitating role on creative behaviors

The goal of this study is to explore how experiencing flow with IM, an Internet-based communication technology, can facilitate an individual’s perception on creativity. We argue that certain capabilities in IM, telepresence and perceived control, provide users a means to experience flow. We further investigate whether flow and its outcomes, positive affect and exploratory behavior have any influence on the perception of creativity of the users. Using an online survey with 207 observations we found that user’s perceived control over the technology and its ability to transport the user to a virtual environment were shown to facilitate the experience of flow. We found that flow in itself does not influence perceived expected creativity in its users but does indirectly influence perceived expected creativity through exploratory behavior and positive affect. From these initial findings we believe that our study provides implications for both users and designers on IT. Furthermore, managers can apply the findings during implementation and use of interactive information technologies for communications in the workplace.     

Identifying high perceived value practices of CMMI level 2: An empirical study

ObjectiveIn this paper, we present findings from an empirical study that was aimed at identifying the relative “perceived value” of CMMI level 2 specific practices based on the perceptions and experiences of practitioners of small and medium size companies. The objective of this study is to identify the extent to which a particular CMMI practice is used in order to develop a finer-grained framework, which encompasses the notion of perceived value within specific practices.MethodWe used face-to-face questionnaire based survey sessions as the main approach to collecting data from 46 software development practitioners from Malaysia and Vietnam. We asked practitioners to choose and rank CMMI level 2 practices against the five types of assessments (high, medium, low, zero or do not know). From this, we have proposed the notion of ‘perceived value’ associated with each practice.ResultsWe have identified three ‘requirements management’ practices as having a ‘high perceived value’. The results also reveal the similarities and differences in the perceptions of Malaysian and Vietnamese practitioners with regard to the relative values of different practices of CMMI level 2 process areas.ConclusionsSmall and medium size companies should not be seen as being “at fault” for not adopting CMMI – instead the Software Process Improvement (SPI) implementation approaches and its transition mechanisms should be improved. We argue that research into “tailoring” existing process capability maturity models may address some of the issues of small and medium size companies.     

A new spatial spherical pattern model into interactive cartography pattern: multi-dimensional data via geostrategic cluster

A growing amount of research conducted in digital, cooperative with advances in Artificial Intelligence, Computer Vision including Machine learning, has managed to the advance of progressive techniques that aim to detect and process affective information contained in multi-modal evidences. This research intends to bring together for theoreticians and practitioners from academic fields, professionals and industries and extends to be visualizing cries such epidemic, votes, social Phenomena in spherical representation interactive model working in the broad range of topics relevant to multi - modal data processing and forensics tools developing. Furthermore, progress has been made in this research besides that in this research conducted progression of mapping claims in present epoch necessitate the capacities of virtual guide of any understandable Geo-Visualization of spatial features that talented to convert the quantities of spatial pattern into cartography. The enlargement of a novel approaches fit for visualization of spatial pattern constituencies Starting exclusive Input Set of object O, set associated with feature F for regenerating Output the set C , interested region I special target C Even so, as indicated by the construction of the prototype as listed earlier in this thread, does it have the incentive for improvements: Representation could be used by Google Earth can Using Project enhancement representation whereby provides a 3D or 4D interaction with life measures with a view to cartography. In addition, the initiative suggests that a tool not accessible for disseminating information to the public can be addressed by the use of online mapping, which fuses with trends visualization for political circles and electors. But as mentioned above the framework is developed and it's also possible in the current example, for improvements: The project's representation 3D or 4D interacting Earth can use measures of life Earth From the map viewpoint. That's what that says. That means that. Which just means. Developers have concerns that. So it. Designers concern about that. This study supports the new, multi - demission and deployed countries in conjunction with another data is processed. Comprehensive, well-interpreted source data for the Data like Malaysia Jabatan Pendaftaran (JPN).