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Lopez-Canovas L Bravo L Herrera J Riveron AM Javer E Sanchez A Fando R Noa MD Fernandez A 《Electrophoresis》2006,27(14):2857-2864
DNA molecules of Vibrio cholerae and Aeromonas species were prepared by incubating immobilized cells for 4 and 2 h, respectively, with a nonenzymatic solution that contains chemical reagents only (NDSUPlus). This method gave results as reproducible as the enzymatic one that uses proteinase K, and rendered DNA molecules suitable for fingerprinting by mini-CHEF electrophoresis. As rapid DNA separations at high electric field are achieved in mini-CHEF chamber with low heat evolution, DNA restriction fragments were separated in 5 h at 10 V/cm in a single resolution window. Then, fragment separations in three resolution windows were done in 15 h. This time is shorter than the one needed by the large CHEF chamber for resolving fragments in a single resolution window. Three windows permitted to include larger numbers of restriction fragments in the calculation of isolate similarities. Both sample preparation and mini-CHEF electrophoresis may represent an alternative for performing massive epidemiological studies of V. cholerae and Aeromonas species. 相似文献
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Alipov A. A. Lekontseva N. V. Mikhailina A. O. Fando M. S. Tishchenko S. V. Nikulin A. D. 《Crystallography Reports》2021,66(5):791-796
Crystallography Reports - The bacterial protein Hfq is a member of a large family of Lsm proteins, which are characterized by a conserved tertiary structure and form a stable ring-shaped quaternary... 相似文献
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Vladislav V. Fomenko Nadezhda B. Rudometova Olga I. Yarovaya Artem D. Rogachev Anastasia A. Fando Anna V. Zaykovskaya Nina I. Komarova Dmitry N. Shcherbakov Oleg V. Pyankov Andrey G. Pokrovsky Larisa I. Karpenko Rinat A. Maksyutov Nariman F. Salakhutdinov 《Molecules (Basel, Switzerland)》2022,27(1)
When developing drugs against SARS-CoV-2, it is important to consider the characteristics of patients with different co-morbidities. People infected with HIV-1 are a particularly vulnerable group, as they may be at a higher risk than the general population of contracting COVID-19 with clinical complications. For such patients, drugs with a broad spectrum of antiviral activity are of paramount importance. Glycyrrhizinic acid (Glyc) and its derivatives are promising biologically active compounds for the development of such broad-spectrum antiviral agents. In this work, derivatives of Glyc obtained by acylation with nicotinic acid were investigated. The resulting preparation, Glycyvir, is a multi-component mixture containing mainly mono-, di-, tri- and tetranicotinates. The composition of Glycyvir was characterized by HPLC-MS/MS and its toxicity assessed in cell culture. Antiviral activity against three strains of SARS-CoV-2 was tested in vitro on Vero E6 cells by MTT assay. Glycyvir was shown to inhibit SARS-CoV-2 replication in vitro (IC502–8 μM) with an antiviral activity comparable to the control drug Remdesivir. In addition, Glycyvir exhibited marked inhibitory activity against HIV pseudoviruses of subtypes B, A6 and the recombinant form CRF63_02A (IC50 range 3.9–27.5 µM). The time-dependence of Glycyvir inhibitory activity on HIV pseudovirus infection of TZM-bl cells suggested that the compound interfered with virus entry into the target cell. Glycyvir is a promising candidate as an agent with low toxicity and a broad spectrum of antiviral action. 相似文献
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