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重组人表皮生长因子(rhEGF)已经广泛应用于医疗和化妆品。以杆状病毒AnpeNPV为基因表达载体,昆虫为生物反应器而建立的蛋白表达系统已获得成功。本文以人表皮生长因子(hEGF)基因替代柞蚕AnpeNPV中的核多角体基因而获得的AnpehEGF病毒为载体、蓖麻蚕蛹为生物反应器表达的rhEGF蛋白质,采用PCR、West-ern blot和ELISA等实验方法对其进行检测,硫酸铵沉淀和Ni-NTA Agrose亲和层析法对其进行分离纯化。实验结果显示,无论是在基因或是蛋白水平上都可检测到rhEGF的表达。AnpehEGF感染蚕蛹后第6d开始检测到rhEGF的表达量快速上升,第12d达到高峰,第3、6、9、12d的表达量分别为19.77、24.90、618.59、1 952.46ng/g,而到了病毒感染后期(第15d)出现了蛋白降解现象。表达的rhEGF通过分离纯化获得了较纯的产品。结果表明蓖麻蚕蛹作为生物反应器生产外源蛋白rhEGF是可行的,说明利用AnpeNPV和蓖麻蚕蛹可开发更加低成本而又高效的rhEGF生产新途径。 相似文献
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Wang Yi-lan王穆兰 Lu Pei-kun陆培坤 Shao Min邵民 Chen Shou-chun陈寿椿 Wu Yu-ting伍玉婷 Zheng Zhen-ni郑振妮 Ren Yue-xian任月仙 《中华医学杂志(英文版)》1984,97(9):631-638
We conducted a field survey on 53 workers in
a battery factory, 52 solder workers, and 50
embroidery workers (control group). The average
air lead levels at the workplaces of these three groups
were 0.578 mg/nt, 0.0015 mg/m:3 and 0.006 mg,iml
respectively. Clinical and biochemical studies re
vealed that the workers in Shanghai Navigation
Battery Factory were affected somewhat by the toxic
effects of lead. The solder workers showed no signi-
ficant abnormalities compared with the control
group. A significant dose-response relationship ex
isted between air lead levels and toxic effects.
The early clinical manifestations observed were
dysfunction of the central nervous system, dyspcpsia,
joint pain, and myalgia in the extremities. A positive
association was observed between the prevalences of
fatigue, mild abdominal pain, and joint pain and
the blood lead (PbB), urine lead (PbU), and zinc
protoporphyrin (ZPP) levels. The symptomatic
threshold values for PbB, PbU, and ZPP were 30
u.g/dl, 0.045 mg/'I, and 40 Ug/dl, respectively
Although the PbB, PbU, 6-aminolevulinic acid
dehydratase (ALAD), free erythrocyte protoporphyrin
(FEP), and ZPP levels could be used as criteria of
lead exposure, the measurement of ZPP is the first-
choice screening test for a preventive monitoring
program.
The motor and sensory conduction velocities in
the median nerve were slower in the exposure group
than the control group (p<0.05). Conduction velocity
and intensity of lead exposure were not significantly
correlated. No apparent effects on behavioral func
tion were observed among the solder workers. 相似文献
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目的探讨蓖麻蚕蛹生物反应器中分离纯化获得的重组人表皮生长因子(rhEGF)蛋白质的生物学活性。方法采用Tricine-SDS-PAGE和免疫印迹法(Western blot)鉴定rhEGF,通过MTT和台盼蓝法检测rhEGF的细胞活性。结果 Tricine-SDS-PAGE、Western blot结果显示,蓖麻蚕蛹中分离纯化获得的rhEGF具有正确的分子量和免疫原性;MTT法显示,纯化的rhEGF具有酶促活性,且酶促活性优于rhEGF标准蛋白(P<0.05),其最高活性浓度为1.0μg/L,在0.0625~1.0μg/L浓度范围内具有剂量依赖性;rhEGF能够促进Balb/c3T3细胞的分裂增殖,效果优于rhEGF标准蛋白(P<0.05)。结论 AnpehEGF感染的蓖麻蚕蛹中分离纯化获得的rhEGF,具有类似天然hEGF的生物学活性,且效果优于rhEGF标准蛋白;rhEGF的细胞实验为进一步的动物实验提供了基础。 相似文献
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本文检测了职业铅接触者的周围神经传导速度。与未接触铅的对照组相比,接触组正中神经运动和感觉传导速度明显降低。研究表明,神经传导速度与血铅浓度有关,当血铅值大于70μg/100ml时,二组差别有显著意义。以上提示,铅对周围神经有一定损害,在预防上应予以足够的重视。 相似文献
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职业性铅接触危害的研究 总被引:4,自引:0,他引:4
本文调查了接触铅烟平均浓度为0.578mg/m~3的蓄电池厂工人53名,0.0015mg/m~3的小焊锡工52名以及0.0006mg/m~3的对照组绣花工50名。从临床症状与生化指标来看,蓄电池厂工人已受到了一定程度的铅危害,小焊锡工与对照组工人相比未见异常,可见铅的毒性危害呈明显的剂量-反应关系。铅的毒性早期症状为中枢神经系统功能紊乱、消化不良和四肢肌肉关节酸痛等。本文见乏力、腹部隐痛和关节酸痛的发生数与PbB、PbU及ZPP的浓度成正比,其症状阈浓度分别为30μg/dl、0.045mg/l及40μg/dl。铅接触的指标可以采用PbB、PbU、ALAD、FEP及ZPP,但作为预防性监护的过筛指标以ZPP为宜。铅接触组的正中神经运动及感觉传导速度均较对照组为慢(P<0.05)。在铅接触组工人中,传导速度的减慢与铅接触程度未见明显关系。 相似文献