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Alfredo M. Gravagnuolo Eden Morales‐Narváez Charlene Regina Santos Matos Sara Longobardi Paola Giardina Arben Merkoçi 《Advanced functional materials》2015,25(38):6084-6092
Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal protein, is known to self‐assemble into chemically stable amphiphilic films, to be able to change wettability of surfaces, and to strongly adsorb other proteins. Herein, a fast, highly homogeneous and efficient glass functionalization by spontaneous self‐assembling of Vmh2 at liquid–solid interfaces is achieved (in 2 min). The Vmh2‐coated glass slides are proven to immobilize not only proteins but also nanomaterials such as graphene oxide (GO) and quantum dots (QDs). As models, bovine serum albumin labeled with Alexa 555 fluorophore, anti‐immunoglobulin G antibodies, and cadmium telluride QDs are patterned in a microarray fashion in order to demonstrate functionality, reproducibility, and versatility of the proposed substrate. Additionally, a GO layer is effectively and homogeneously self‐assembled onto the studied functionalized surface. This approach offers a quick and simple alternative to immobilize nanomaterials and proteins, which is appealing for new bioanalytical and nanobioenabled applications. 相似文献
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Simonetta Balsamo Vittoria De Nitto Person Paola Inverardi 《Performance Evaluation》2003,51(2-4):269-288
A review is carried out on how queueing network models with blocking have been applied so far into the performance evaluation and prediction of Software Architectures (SA). Queueing network models with finite capacity queues and blocking have recently been introduced and applied as more realistic models of systems with finite capacity resources and population constraints. Queueing network models have been often adopted as models for the evaluation of software performance. Starting from our own experience, we observe the need of a more accurate definition of the performance models of SA to capture some features of the communication systems. We consider queueing networks with finite capacity and blocking after service (BAS) to represent some synchronization constraints that cannot be easily modeled with queueing network models with infinite capacity queues. We investigate the use of queueing networks with blocking as performance models of SA with concurrent components and synchronous communication. Queueing theoretic analysis is used to solve the queueing network model and study the synchronous communication and performance of concurrent software components. Our experience is supported by other approaches that also propose the use of queueing networks with blocking. Directions for future research work in the field are included. 相似文献
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In the present work, the ohmic resistance of an integrated planar-SOFC (IP-SOFC) has been evaluated by developing a model
whose equations have been solved numerically through an FEM method. The model allows to estimate the distribution of voltage
and current density in the cell. A comparison between simulated and experimental data of area specific resistance is reported,
which shows satisfactory agreement. The mathematical model has also been used to carry out some parametric studies for optimisation
purposes. Indeed, a reduction in cell pitch length and an increase in electrode thickness are predicted to lead to a reduction
in ohmic losses in IP-SOFCs. 相似文献
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Giampiero Sacchetti Paola Pittia Manuela Biserni Gian G. Pinnavaia & Marco D. Rosa 《International Journal of Food Science & Technology》2003,38(2):135-143
Summary Ready‐to‐eat breakfast cereals immersed in milk undergo undesirable changes in texture because of sudden moisture uptake. The textural changes are ascribable to a plasticizing effect of water, which modifies the mechanical strength of products by softening the starch/protein matrix. In this work, some textural parameters of different cereal flakes were derived from the force–displacement curves monitored during 300 s of immersion in milk. Hardness loss, deformability increment and changes in the force–displacement curve profile were calculated and plotted against soaking time. The application of a sugar coating process to a model cereal flake increased the initial product hardness and improved the preservation of the textural parameters during immersion. The Peleg model closely fitted the experimental data, with regression coefficients from 0.967 to 0.999. 相似文献
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Giovannetti Antonio Susi Gianluca Casti Paola Mencattini Arianna Pusil Sandra López María Eugenia Di Natale Corrado Martinelli Eugenio 《Neural computing & applications》2021,33(21):14651-14667
Neural Computing and Applications - In this paper, we present the novel Deep-MEG approach in which image-based representations of magnetoencephalography (MEG) data are combined with ensemble... 相似文献
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Marta Guerini Pietro Grisoli Cristina Pane Paola Perugini 《International journal of molecular sciences》2021,22(2)
The aim of this work was the development of microstructured lipid carriers (MLC) based on chitosan (CH) and containing N-acetylcysteine (NAC), a mucolytic and antioxidant agent, to inhibit the formation of Pseudomonas aeruginosa biofilm. MLC were prepared using the high shear homogenization technique. The MLC were characterized for morphology, particle size, Z potential, encapsulation efficiency and drug release. The antioxidant properties of NAC-loaded microstructured carriers were evaluated through an in vitro spectrophotometer assay. Finally, the activity of NAC-CH-MLC on biofilm production by Pseudomonas aeruginosa was also evaluated. Results obtained from this study highlighted that the use of chitosan into the inner aqueous phase permitted to obtain microstructured particles with a narrow size range and with good encapsulation efficiency. NAC-loaded MLC showed higher antioxidant activity than the free molecule, demonstrating how encapsulation increases the antioxidant effect of the molecule. Furthermore, the reduction of biofilm growth resulted extremely high with MLC being 64.74% ± 6.2% and 83.74% ± 9.95%, respectively, at 0.5 mg/mL and 2 mg/mL. In conclusion, this work represents a favorable technological strategy against diseases in which bacterial biofilm is relevant, such as cystic fibrosis. 相似文献