Relative involvement of protein kinase C and of the estrogen receptor in the cytotoxic action of a population of triphenylethylenes on MCF7 cells as revealed by correspondence factorial (CF) analysis

A multivariate statistical method, correspondence factorial (CF) analysis, was used to examine the correlations among the protein binding and cell proliferation effects of a series of 36 di- and triphenylethylenes (DPEs and TPEs). The analysis was applied to a study which measured their competition for estradiol binding to cytosol estrogen receptor (ER), their influence on protein kinase C (PKC) activity under different conditions of enzyme activation, their ability to promote the growth of a breast cancer cell line and to inhibit growth at high concentrations (cytotoxicity). The CF analysis revealed several levels of correlation. First, it distinguished those molecules within the population that stimulated rather than inhibited PKC activity. Second, it made apparent a strong correlation between cytotoxicity and inhibition of Ca++ and phosphatidylserine-dependent PKC activity, which was most marked when the enzyme had been activated by diacylglycerol indicating that PKC inhibition under physiological conditions might contribute to the overall cytotoxicity of these compounds. Third, a lower level of correlation was established between competition for ER binding and cytotoxicity. Taken together, the results suggest that MCF7 cells might be most sensitive to a cytotoxic effect of TPEs (via PKC and other targets) when they at the same time decrease estrogen-stimulated proliferation via an ER-mediated antiestrogenic effect.     

Effects of allelochemicals on plant respiration and oxygen isotope fractionation by the alternative oxidase

The goal of this investigation was to determine the effects of allelochemicals on plant respiration that thereby may be responsible for their role in growth inhibition. We have tested the effects of juglone, quercetin, cinnamic acid, and-pinene on respiration rates, and electron partitioning through the cytochrome and alternative respiratory pathways, by measuring on-line oxygen consumption and oxygen isotope fractionation in soybean cotyledon tissue. Cinnamic acid and-pinene decreased the oxygen consumption rate and increased the relative partitioning of electron transport to the alternative pathway. Possible biochemical mechanisms of these effects are discussed.     

Discovering social structures of local influence by using tweetStimuli

Information diffusion in large-scale networks has been studied to identify the users influence. The influence has been targeted as a key feature either to reach large populations or influencing public opinion. Through the use of micro-blogs, such as Twitter, global influencers have been identified and ranked based on message propagation (retweets). In this paper, a new application is presented, which allows to find first and classify then the local influence on Twitter: who have influenced you and who have been influenced by you. Until now, social structures of tweets’ original authors that have been either retweeted or marked as favourites are unobservable. Throughout this application, these structures can be discovered and they reveal the existence of communities formed by users of similar profile (that are connected among them) interrelated with other similar profile users’ communities.     

Solving weighted CSPs with meta-constraints by reformulation into satisfiability modulo theories

We introduce WSimply, a new framework for modelling and solving Weighted Constraint Satisfaction Problems (WCSP) using Satisfiability Modulo Theories (SMT) technology. In contrast to other well-known approaches designed for extensional representation of goods or no-goods, and with few declarative facilities, our approach aims to follow an intensional and declarative syntax style. In addition, our language has built-in support for some meta-constraints, such as priority and homogeneity, which allows the user to easily specify rich requirements on the desired solutions, such as preferences and fairness. We propose two alternative strategies for solving these WCSP instances using SMT. The first is the reformulation into Weighted SMT (WSMT) and the application of satisfiability test based algorithms from recent contributions in the Weighted Maximum Satisfiability field. The second one is the reformulation into an operation research-like style which involves an optimisation variable or objective function and the application of optimisation SMT solvers. We present experimental results of two well-known problems: the Nurse Rostering Problem (NRP) and a variant of the Balanced Academic Curriculum Problem (BACP), and provide some insights into the impact of the addition of meta-constraints on the quality of the solutions and the solving time.     

The efforts in the forearm during the use of anti-vibration gloves in simulated work tasks

The objective of this article is to analyze the levels of forearm muscular contraction associated with the use of anti-vibration gloves, in which the contraction levels with gloves and without gloves are compared. Two different vibrating tools - a Bosch Compact Duty Multi-Cutter and a Bosch Rotary Hammer carrying an Ø20 mm bit - were used in a simulated work environment. Standard operations were performed by each subject (n = 14) in an 80 × 40 mm pine bar (cross-sectional cuts with the Multi-Cutter) and on a concrete slab 2 × 2 m, 70 mm thick (20 mm holes with the Rotary Hammer). The forearm muscular efforts were measured by surface electromyography (EMG) in four different muscles: the flexor digitorum superficialis (FDS), flexor carpi ulnaris (FCU), extensor carpi radialis longus (ECRL) and extensor carpi ulnaris (ECU). For the flexor muscles (FDS and FCU), a decrease tendency (p > 0.05) in the measured EMG was observed when the operations are performed with gloves relative to the bare hand (a reduction of 5-23% in the percentage of maximum voluntary exertion (%MVE)). For the extensor muscles (ECU), a tendency toward increased (p > 0.05) muscular contraction was observed when the operations were performed with gloves (an increase of 3-20% in the %MVE). No such tendency was found in the ECRL muscle. The ECU was the muscle with the highest %MVE for 10 and 11 operators (n = 14), during the operations, respectively, with the Multi-Cutter and the Rotary Hammer. As a final conclusion from the study, anti-vibration gloves may increase forearm fatigue in the posterior forearm (ECU muscle) and decrease forearm fatigue in the FDS muscle during operations with the above-mentioned tools.

Relevance to industry

Anti-vibration gloves have been applied in industry to reduce the vibration transmitted into the hand and arms through the palms and fingers. This study analyzed forearm muscular efforts during simulated work tasks performed with two different vibrating tools, operated with the bare hand and with three different gloves, based on the analysis of EMG data.     

A Systematic Study of Restorative Crown-Materials Combinations for Dental Implants: Characterization of Mechanical Properties under Dynamic Loads

This study aimed to find the optimum mechanical characteristics of the restorative materials for the manufacture of implant crowns subjected to impact loading when different combinations of materials are used for the inner and outer crown. Several combinations of external–internal crown restorative materials were analyzed. The dynamic stresses at eight different zones of a dental implant subjected to an impact load and the influence of several mechanical properties, such as the Young’s modulus, Poisson’s ratio, density, and initial velocity, were analyzed and compared. A detailed 3D model was created, including the crown, the retention screw, the implant, and a mandible section. The model was then built by importing the 3D geometries from CAD software. The whole 3D model was carefully created in order to guarantee a finite element mesh that produced results adjusted to physical reality. Then, we conducted a numerical simulation using the finite element method (FEM). The results of the FEM analysis allowed for evaluating the effect that different combinations of restorative materials and mechanical properties had on the stress distribution in various regions of the implant. The choice of restorative material is a factor to be considered in order to preserve the integrity of osseointegration. Restorative materials transfer more or less stress to the dental implant and surrounding bone, depending on their stiffness. Therefore, an inadequate Young’s modulus of the rehabilitation material can affect the survival of the implant over time. Eight interactive graphics were provided on a web-based surface platform to help clinical dentists, researchers, and manufacturers to select the best restorative materials combination for the crown.     

Parallel enzymatic and non-enzymatic formation of zinc protoporphyrin IX in pork

Zinc protoporphyrin is formed in pork homogenates in both enzymatic and non-enzymatic reactions. Ferrochelatase is active in formation of the highly fluorescent pigment known from Parma ham as demonstrated by inhibition with N-methylmesoporphyrin and by thermal inactivation. A non-enzymatic transmetallisation reaction, exchange of iron in myoglobin by zinc(II), is demonstrated by Pb(II) inhibition of zinc protoporphyrin formation at low Pb(II) concentrations, but promoted at higher Pb(II) concentrations. The non-enzymatic reaction is characterised as a slow bimolecular reaction between protoporphyrin IX and zinc(II) with a second-order rate constant of 0.63 l mol⁻¹ s⁻¹ at 35 °C and a high energy of activation of 98 kJ mol⁻¹ for acetone:water (3:1, v/v) as solvent. Zinc protoporphyrin formation is concluded to be thermodynamically controlled with a formation constant of 4 × 10⁵ M (35 °C, acetone:water (3:1)). An efficient inhibition of formation of zinc protoporphyrin by nitrite is related to myoglobin as substrate and involves both enzymatic and non-enzymatic reactions.     

Resilience to increasing temperatures: residential building stock adaptation through codes and standards

The resilience of the current Spanish residential building stock to increased temperatures is modelled. Homogenized daily temperature data recorded at 50 Spanish meteorological stations for the periods 1950–1979 and 1981–2010 were used to investigate anticipated climate warming on the Spanish residential building stock by means of the degree-day method. Impacts on residential buildings were investigated for three different future time periods (2011–2040, 2041–2070 and 2071–2100) for three representative Spanish provincial capitals. Future climate change scenarios comprising two statistical downscaling methods, three general circulation models and two carbon emission scenarios were used to project local climate. Results show that 72% of current residential building stock in Spain is thermally unprotected. In addition, the energy demand for heating the building sector in Spain is expected to decrease by between 30% (Barcelona, B2 scenario) and 36% (Valencia, A2 scenario) by 2100, while the respective energy demand for cooling could increase by between 107% (Valencia, B2 scenario) and 296% (Madrid, A2 scenario) by 2100. To increase resilience to higher winter and summer temperatures, strategies for modifying the built environment are needed, particularly for the role of building codes and standards.

La résilience du parc bâti résidentiel espagnol actuel face à l'augmentation des températures est modélisée. Les données homogénéisées des température journalières enregistrées dans 50 stations météorologiques espagnoles pour les périodes 1950–1979 et 1981–2010 ont été utilisées pour étudier le réchauffement climatique prévu sur le parc bâti résidentiel espagnol en utilisant la méthode des degrés-jours. Les répercussions sur les immeubles résidentiels ont été étudiées pour trois périodes futures différentes (2011–2040, 2041–2070 et 2071–2100) et pour trois capitales provinciales espagnoles représentatives. Il a été utilisé des scénarios de changement climatique futur comprenant deux méthodes de réduction d'échelle statistique, trois modèles de circulation générale et deux scénarios d'émission de carbone pour prévoir le climat local. Les résultats montrent que 72 % du parc bâti résidentiel actuel de l'Espagne est dépourvu de protection thermique. Il est en outre prévu que la demande énergétique liée au chauffage dans le secteur du logement en Espagne diminue de 30 % (Barcelone, scénario B2) à 36 % (Valence, scénario A2) d'ici à 2100, tandis que la demande énergétique respective liée à la climatisation pourrait augmenter de 107 % (Valence, scénario B2) à 296 % (Madrid, scénario A2) d'ici à 2100. Afin d'augmenter la résilience face à des températures hivernales et estivales plus élevées, il est nécessaire de disposer de stratégies de modification du cadre bâti, concernant en particulier le rôle des codes et des normes du bâtiment.

Mots clés: stratégies d'adaptation, performances des bâtiments, réglementation du bâtiment, parc bâti, changement climatique, degrés-jours, surchauffe, confort thermique, vulnérabilité, Espagne     

Genetic and management factors affecting beef quality in grazing Hereford steers

Attributes contributing to differences in beef quality of 206 Hereford steers finished on pasture were assessed. Beef quality traits evaluated were: Warner-Bratzler meat tenderness and muscle and fat color at one and seven days after slaughter and trained sensory panel traits (tenderness, juiciness, flavor, and marbling) at seven days. Molecular markers were CAPN1 316 and an SNP in exon 2 on the leptin gene (E2FB). Average daily live weight gain, ultrasound monthly backfat thickness gain and rib-eye area gain were estimated. Molecular markers effects on meat quality traits were analyzed by mixed models. Association of meat quality with post weaning growth traits was analyzed by canonical correlations. Muscle color and marbling were affected by CAPN1 316 and E2FB and Warner-Bratzler meat tenderness by the former. The results confirm that marker assisted selection for tenderness is advisable only when beef aging is a common practice. The most important sources of variation in tenderness and color of meat remained unaccounted for.