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Anna Iwaniak Damir Mogut Piotr Minkiewicz Justyna ulewska Magorzata Darewicz 《International journal of molecular sciences》2021,22(6)
In silico and in vitro methods were used to analyze ACE- and DPP-IV-inhibiting potential of Gouda cheese with a modified content of β-casein. Firstly, the BIOPEP-UWM database was used to predict the presence of ACE and DPP-IV inhibitors in casein sequences. Then, the following Gouda cheeses were produced: with decreased, increased, and normative content of β-casein after 1 and 60 days of ripening each (six variants in total). Finally, determination of the ACE/DPP-IV-inhibitory activity and the identification of peptides in respective Gouda-derived water-soluble extracts were carried out. The identification analyses were supported with in silico calculations, i.e., heatmaps and quantitative parameters. All Gouda variants exhibited comparable ACE inhibition, whereas DPP-IV inhibition was more diversified among the samples. The samples derived from Gouda with the increased content of β-casein (both stages of ripening) had the highest DPP-IV-inhibiting potency compared to the same samples measured for ACE inhibition. Regardless of the results concerning ACE and DPP-IV inhibition among the cheese samples, the heatmap showed that the latter bioactivity was predominant in all Gouda variants, presumably because it was based on the qualitative approach (i.e., peptide presence in the sample). Our heatmap did not include the bioactivity of a single peptide as well as its quantity in the sample. In turn, the quantitative parameters showed that the best sources of ACE/DPP-IV inhibitors were all Gouda-derived extracts obtained after 60 days of the ripening. Although our protocol was efficient in showing some regularities among Gouda cheese variants, in vivo studies are recommended for more extensive investigations of this subject. 相似文献
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Justyna Korczynska Aleksandra Czumaj Michal Chmielewski Julian Swierczynski Tomasz Sledzinski 《International journal of molecular sciences》2021,22(9)
Leptin is an adipokine that regulates appetite and body mass and has many other pleiotropic functions, including regulating kidney function. Increased evidence shows that chronic kidney disease (CKD) is associated with hyperleptinemia, but the reasons for this phenomenon are not fully understood. In this review, we focused on potential causes of hyperleptinemia in patients with CKD and the effects of elevated serum leptin levels on patient kidney function and cardiovascular risk. The available data indicate that the increased concentration of leptin in the blood of CKD patients may result from both decreased leptin elimination from the circulation by the kidneys (due to renal dysfunction) and increased leptin production by the adipose tissue. The overproduction of leptin by the adipose tissue could result from: (a) hyperinsulinemia; (b) chronic inflammation; and (c) significant lipid disturbances in CKD patients. Elevated leptin in CKD patients may further deteriorate kidney function and lead to increased cardiovascular risk. 相似文献
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Diana M. Bobrowska Justyna Czyrko Krzysztof Brzezinski Luis Echegoyen 《Fullerenes, Nanotubes and Carbon Nanostructures》2017,25(3):185-192
Carbon nano-onion/surfactant (CNO/surfactant) composites offer the possibility to easily produce the soluble nanostructures. That approach combines the hydrophilicity of surfactants with the robustness of carbon structures to produce composites with superior and unusual physicochemical properties. We used the following surfactants: hexadecyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS), 4-(1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol (Triton X-100), and polyethylene glycol sorbitan monolaurate (Tween 20) to non-covalently modify CNO surfaces. The existence of stable CNO composites are clearly evidenced by direct transmission electron microscopy observations, which are also supported by thermogravimetric analyses. Dynamic light scattering and zeta potential confirmed their dispersion and stability. Additionally, the biological activity of well-dispersed CNO/surfactant composites against a strain of Escherichia coli was assayed. In vitro antimicrobial assays for the composites revealed that only the CNO/CTAB composite decreased cell viability. This activity could be assigned to the simple composite dissociation in water solutions, however antimicrobial properties of the composite are slightly better when compared with pure CTAB. This indicate some synergic effect with respect to the properties of the pure surfactant. 相似文献
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Karolina Dydak Adam Junka Grzegorz Nowacki Justyna Paleczny Patrycja Szymczyk-Zikowska Aleksandra Grzyska Olga Anioek Marzenna Bartoszewicz 《International journal of molecular sciences》2022,23(9)
Hernia repairs are the most common abdominal wall elective procedures performed by general surgeons. Hernia-related postoperative infective complications occur with 10% frequency. To counteract the risk of infection emergence, the development of effective, biocompatible and antimicrobial mesh adjuvants is required. Therefore, the aim of our in vitro investigation was to evaluate the suitability of bacterial cellulose (BC) polymer coupled with gentamicin (GM) antibiotic as an absorbent layer of surgical mesh. Our research included the assessment of GM-BC-modified meshes’ cytotoxicity against fibroblasts ATCC CCL-1 and a 60-day duration cell colonisation measurement. The obtained results showed no cytotoxic effect of modified meshes. The quantified fibroblast cells levels resembled a bimodal distribution depending on the time of culturing and the type of mesh applied. The measured GM minimal inhibitory concentration was 0.47 µg/mL. Results obtained in the modified disc-diffusion method showed that GM-BC-modified meshes inhibited bacterial growth more effectively than non-coated meshes. The results of our study indicate that BC-modified hernia meshes, fortified with appropriate antimicrobial, may be applied as effective implants in hernia surgery, preventing risk of infection occurrence and providing a high level of biocompatibility with regard to fibroblast cells. 相似文献
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Justyna Zygmuntowicz Paweł Falkowski Marcin Wachowski Konrad Cymerman Paulina Piotrkiewicz Waldemar Kaszuwara 《International Journal of Applied Ceramic Technology》2020,17(4):1731-1741
In the present research, the influence of sintering temperature on the microstructure and properties of Al2O3–Cu–Ni hybrid composites prepared by the Pulse Plasma Sintering (PPS) technique were described. In this research, three temperatures have been selected: 1250°C, 1300°C, and 1350°C. SEM observations were carried out to determine the distribution of the metallic phase in the composite depending on the sintering temperature. The conducted experiments and microscopic observations enabled a better understanding of the phenomena occurring between the ceramic matrix and metallic phase in the obtained materials. The mechanical properties like a hardness and fracture toughness were measured. The technology applied allowed us to obtain ceramic-metal composites with a homogeneous microstructure. It was found that the sintering temperature influences the selected physical and mechanical properties of the composites produced. It was found that samples produced at 1300°C are characterized by the highest relative density and the mechanical properties. 相似文献
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Justyna Chodkowska-Miszczuk Daniela Szymańska 《Energy Sources, Part A: Recovery, Utilization, and Environmental Effects》2016,38(1):117-123
The article aims to analyze the current state and determine the perspectives for oilseed rape production for energy purposes in Poland. According to the study, in recent years the oilseed rape area for energy purposes and the production of biocomponents and biofuels from oilseed rape have been on the rise in Poland. The considerable and spatially concentrated energy potential of oilseed rape in Poland is noted. It is indicated that the greatest energy potential of the oilseed rape production is the four neighboring voivodeships: Dolno?l?skie, Opolskie, Wielkopolskie, and Kujawsko-Pomorskie. 相似文献
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Maria Tynek Roman Pawłowicz Justyna Gromadzka Robert Tylingo Waldemar Wardencki Gyorgy Karlovits 《European Journal of Lipid Science and Technology》2012,114(3):357-366
The aim of the present study was to compare different rape varieties. For this purpose oil from six different varieties of rapeseeds was cold pressed under laboratory conditions. In the obtained rapeseed oils the fatty acids composition and minor components, characteristic values (acid value; AV and TOTOX), oxidative stability (DSC test), and volatiles were determined and a sensory evaluation was carried out. The highest oxidative stability was found for oil from sample 5 (IP = 158 min), which also has the lowest amount of C18:3 (7.8%), chlorophylls (0.083 mg/kg), and metals (Cu2+ 0.02 mg/kg and Fe2+ 0.08 mg/kg). This oil has also the lowest AV (0.17 mg KOH/g), which may be related to the lowest moisture content of the seeds prior to extraction. It was characterized by the highest rapeseed flavor intensity. The lowest induction period was observed for samples 3 and 6 (100 min). Although sample 3 had the same low level of metals as sample 5 and the highest concentration of tocopherols (635 mg/kg), PUFA (33.9%), and AV (1.37 mg KOH/g) it also had the lowest intensity of rapeseed flavor among the analyzed oils. Sample 6, despite its low percentage of PUFA (24.7%), conjugated diens and triens, and the lowest content of total volatiles (0.4Vs), had the highest concentration of metals (Cu2+ 0.04 mg/kg and Fe2+ 0.34 mg/kg). 相似文献
8.
Aleksandra Marciniak Weronika Witak Giuseppina Sabatino Anna Maria Papini Justyna Brasu 《International journal of molecular sciences》2020,21(22)
Somatostatin analogues are useful pharmaceuticals in peptide receptor radionuclide therapy. In previous studies, we analyzed a new bicyclic somatostatin analogue (BCS) in connection with Cu(II) ions. Two characteristic sites were present in the peptide chain: the receptor- and the metal-binding site. We have already shown that this ligand can form very stable imidazole complexes with the metal ion. In this work, our aim was to characterize the intramolecular interaction that occurs in the peptide molecule. Therefore, we analyzed the coordination abilities of two cyclic ligands, i.e., P1 only with the metal binding site and P2 with both sites, but without the disulfide bond. Furthermore, we used magnetic circular dichroism (MCD) spectroscopy to better understand the coordination process. We applied this method to analyze spectra of P1, P2, and BCS, which we have described previously. Additionally, we analyzed the MCD spectra of P3 ligand, which has only the receptor binding site in its structure. We have unequivocally shown that the presence of the Phe-Trp-Lys-Thr motif and the disulfide bond significantly increases the metal binding efficiency. 相似文献
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