Comparison of four RNA extraction methods for the detection of porcine reproductive and respiratory syndrome virus by RT-PCR

We investigated the effect of repeated cold stress (RCS) on the capsaicin-evoked release of glutamate from the primary afferent fibers of the rat, and compared this with the effect of inoculation of complete Freund's adjuvant (adjuvant inoculation). The release of glutamate was measured using a fluorometric on-line continuous monitoring system in which the immobilized glutamate dehydrogenase column was connected to an in vitro superfusion system. In the presence of 0.3 microM tetrodotoxin, the application of 1 microM capsaicin to spinal dorsal horn slices evoked glutamate release (18.6 +/- 1.2 pmol mg(-1) protein, n = 11). In rats subjected to RCS (RCS rats), the release of glutamate evoked by 1 microM capsaicin was markedly increased to 272% (n = 6, P < 0.05) of the value for the control group, although the basal release was not significantly altered (n = 6, P > 0.05). Adjuvant inoculation produced a significant increase in the basal and capsaicin (1 microM) evoked release of glutamate to 141 and 344% (n = 6, P < 0.05) of the value for the control group, respectively. The present results suggest that the facilitated release of glutamate from capsaicin-sensitive primary afferent terminals in the spinal dorsal horn is, at least in part, involved in the hyperalgesia of RCS rats as well as the complete Freund's adjuvant-induced hyperalgesia.     

Simulation and Modeling-implementing GaAs device models with SPICE

A method for evaluating the accuracy of a device model and its tendency toward convergence problems before implementing it as a SPICE primitive is presented. Two different GaAs MESFET models suitable for analyzing nonlinear analog and microwave circuits are implemented using generic SPICE primitives. It is shown that no modification to the SPICE program is required. Controlled sources are extensively used in implementing the models using this method     

Vinyl acetate monomer—a pilot plant study

The kinetics of the reaction of acetic acid and acetylene over zinc acetate-activated carbon catalyst was investigated over a wide range of process variables in a pilot reactor. Although various catalytic reaction mechanisms were postulated, the rate of reaction was most satisfactorily correlated by a mechanism of surface reaction between charged adsorbed acetic acid and acetylene, which assumes that the rate controlling step was the irreversible charged adsorption of acetylene and acetic acid.     

Managing signal integrity [PCB design]

As system clock rates race to 100 MHz and beyond, designers of printed-circuit boards and multichip modules must watch out for such high-speed effects as ground bounce, ringing, reflections, and crosstalk. The author describes how today's high-speed printed-circuit boards and multichip modules require integrated design systems that include signal integrity analysis tools     

Dependence of critical current density on microstructure and processing of high-Tc superconductors

Microstructural origins for reduced weak-link behavior in high-J_c melt-processed YBCO, spray pyrolyzed thick films of Tl-1223, metallic precursor Y-124 polycrystalline powder-in-tube (PIT) wires and PIT Bi-2212/2223 are discussed. Since the materials studied are the highest J_c, polycrystalline, high-T_c superconductors fabricated worldwide, the results provide important guidelines for further improvements in superconducting properties, thereby enabling practical applications of these materials. It is found that strongly linked current flow within domains of melt-processed 123 occurs effectively through a single crystal path. In c-axis oriented, polycrystalline Tl-1223 thick films, local in-plane texture has been found to play a crucial role in the reduced weak-link behavior. Formation of “colonies” of grains with a common c-axis and modest in-plane misorientation was observed. Furthermore, a colony boundary in general has a varying misorientation along the boundary. Large regions comprised primarily of low angle boundaries were observed. Percolative transport through a network of such small angle boundaries appears to provide the nonweak-linked current path. Although powder-in-tube BSCCO 2212 and 2223 also appear to have a “colony” microstructure, there are some important differences. Colonies in BSCCO consist of stacks of grains with similar c-axis orientation in contrast to colonies in Tl-1223 films where few grains are stacked on top of one another. Furthermore, most grains within a colony in BSCCO have the same lateral dimensions as that of the colony, resulting largely largely in “twist” boundaries. Further microstructural characterization of high-J_c PIT 2212 and 2223 is currently underway. In the case of Y-124 wires, weak macroscopic in-plane texture is found. Additional measurements are underway to determine if a sharper, local in-plane texture also exists. It is found that in three of the four types of superconductors studied, reduced weak-link behavior can be ascribed to some degree of biaxial alignment between grains, either on a “local” or a “global” scale.     

Critical current and microstructure in oxide superconductors

Critical current density (J_c) in the presence of a magnetic field is the property that currently limits many applications of the high-critical-temperature oxide superconductors. Poor current transmission at grain boundaries and weak flux pinning are responsible for the low critical current densities observed in bulk materials. Since 1986, substantial progress has been made both in understanding the microstructural factors affecting J_c and in developing practical fabrication methods. Much of the fundamental understanding has been obtained from studies of YBa₂Cu₃O_7?x while the bismuth and thallium-based compounds appear to offer greater potential as the basis for the first practical conductors.     

NMR studies of poly(2-hydroxy ethyl methacrylate-co-2-vinyl pyridine)

Copolymers of 2-hydroxy ethyl methacrylate-2-vinyl pyridine (H/V) of different composition were synthesized by free radical bulk polymerization using azobisisobutyronitrile (AIBN) as an initiator under nitrogen atmosphere. The copolymer compositions were calculated from ¹H NMR spectra. The reactivity ratios for H/V copolymers obtained from a linear Kelen-Tudos method (KT) and nonlinear error-in-variables method (EVM) are r_H = 0.50 ± 0.10, r_V = 1.04 ± 0.08 and r_H = 0.55, r_V = 1.06 respectively. The complete spectral assignment of methine, methylene, methyl, carbonyl, and aromatic carbon regions in term of compositional and configurational sequences of H/V copolymers were done with the help of ¹³C{¹H} NMR, distortionless enhancement by polarization transfer (DEPT), two-dimensional heteronuclear single quantum coherence (HSQC) along with total correlated spectroscopy (TOCSY). © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

Long-term evaluation of the ventricular defibrillation energy requirement

INTRODUCTION: Defibrillation energy requirements in patients with nonthoracotomy defibrillators may increase within several months after implantation. However, the stability of the defibrillation energy requirement beyond 1 year has not been reported. The purpose of this study was to characterize the defibrillation energy requirement during 2 years of clinical follow-up. METHODS AND RESULTS: Thirty-one consecutive patients with a biphasic nonthoracotomy defibrillation system underwent defibrillation energy requirement testing using a step-down technique (20, 15, 12, 10, 8, 6, 5, 4, 3, 2, and 1 J) during defibrillator implantation, and then 24 hours, 2 months, 1 year, and 2 years after implantation. The mean defibrillation energy requirement during these evaluations was 10.9+/-5.5 J, 12.3+/-7.3 J, 11.7+/-5.6 J, 10.2+/-4.0 J, and 11.7+/-7.4 J, respectively (P = 0.4). The defibrillation energy requirement was noted to have increased by 10 J or more after 2 years of follow-up in five patients. In one of these patients, the defibrillation energy requirement was no longer associated with an adequate safety margin, necessitating revision of the defibrillation system. There were no identifiable clinical characteristics that distinguished patients who did and did not develop a 10-J or more increase in the defibrillation energy requirement. CONCLUSION: The mean defibrillation energy requirement does not change significantly after 2 years of biphasic nonthoracotomy defibrillator system implantation. However, approximately 15% of patients develop a 10-J or greater elevation in the defibrillation energy requirement, and 3% may require a defibrillation system revision. Therefore, a yearly evaluation of the defibrillation energy requirement may be appropriate.     

High-utility and diverse itemset mining

High-utility Itemset Mining (HUIM) finds patterns from a transaction database with their utility no less than a user-defined threshold. The utility of an itemset is defined as the sum of the utilities of its items. The utility notion enables a data analyst to associate a profit score with each item and thereof to a pattern. We extend the notion of high-utility with diversity to define a new pattern type called High-utility and Diverse pattern (HUD). The notion of diversity of a pattern captures the extent of the different categories covered by the selected items in the pattern. An application of diverse-pattern lies in the recommendation task where a system can recommend to a customer a set of items from a new class based on her previously bought items. Our notion of diversity is easy to compute and also captures the basic essence of a previously proposed diversity notion. The existing algorithm to compute frequent-diverse patterns is 2-phase, i.e., in the first phase, frequent patterns are computed, out of which diverse patterns are filtered out in the second phase. We, in this paper, give an integrated algorithm that efficiently computes high-utility and diverse patterns in a single phase. Our experimental study shows that our proposed algorithm is very efficient as compared to a 2-phase algorithm that extracts high-utility itemsets in the first phase and filters out the diverse itemsets in the second phase.

     

Processing Dependence of Texture, and Critical Properties of YBa2Cu3O7−δ Films on RABiTS Substrates by a Non-Fluorine MOD Method

YBa₂Cu₃O_{7− δ} (YBCO or Y123) films on rolling-assisted biaxially textured substrates (RABiTS) were prepared via a fluorine-free metallorganic deposition (MOD) through spin coating, burnout, and high temperature anneal. The effects of substrate texture and surface energy of the CeO₂ cap layer were investigated. Except for the commonly accepted key factors, such as the textures of substrate and buffer layers, we found some other factors, for example, the deposition temperature of the cap layer, are also critical to the epitaxial growth of Y123 phase. With the CeO₂ cap layer deposited at relative high temperature of 700°C, a critical current density, J _c, over 1 MA/cm² has been demonstrated for the first time on Ni-RABiTS by a fluorine-free MOD method. Whereas for samples with CeO₂ cap layers deposited at a lower temperature of 600°C, even though XRD data showed a better texture on these buffer layers, texture degradations of YBCO grains under the optimized processing conditions were observed and a lower oxygen partial pressure around 40 ppm was necessary for the epitaxial growth of Y123 phase. As a result, J _c fell to 0.45 MA/cm² at 77 K. The observed phenomena points to the change of surface energy and reactivity of the CeO₂ cap layer with respect to the CeO₂ deposition temperature. In this paper, the YBCO phase diagram was also summarized.