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Amandine Carrër Jean‐Daniel Brion Mouad Alami Samir Messaoudi 《Advanced Synthesis \u0026amp; Catalysis》2014,356(18):3821-3830
A straightforward assisted tandem palladium(II)‐ and palladium(0)‐catalyzed direct C‐3 and N‐4 arylation of quinoxalin‐2(1 H)‐ones with boronic acids and aryl halides in water as safe and cheap solvent is reported. This environmentally friendly catalytic protocol is compatible with a wide range of functional groups and allows construction of various biologically important quinoxalin‐2(1 H)‐one backbones.
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Xavier Vanhoye Alexandre Janin Amandine Caillaud Antoine Rimbert Fabienne Venet Morgane Gossez Wieneke Dijk Oriane Marmontel Sverine Nony Charlotte Chatelain Christine Durand Pierre Lindenbaum Jennifer Rieusset Bertrand Cariou Philippe Moulin Mathilde Di Filippo 《International journal of molecular sciences》2022,23(8)
Hypobetalipoproteinemia is characterized by LDL-cholesterol and apolipoprotein B (apoB) plasma levels below the fifth percentile for age and sex. Familial hypobetalipoproteinemia (FHBL) is mostly caused by premature termination codons in the APOB gene, a condition associated with fatty liver and steatohepatitis. Nevertheless, many families with a FHBL phenotype carry APOB missense variants of uncertain significance (VUS). We here aimed to develop a proof-of-principle experiment to assess the pathogenicity of VUS using the genome editing of human liver cells. We identified a novel heterozygous APOB-VUS (p.Leu351Arg), in a FHBL family. We generated APOB knock-out (KO) and APOB-p.Leu351Arg knock-in Huh7 cells using CRISPR-Cas9 technology and studied the APOB expression, synthesis and secretion by digital droplet PCR and ELISA quantification. The APOB expression was decreased by 70% in the heterozygous APOB-KO cells and almost abolished in the homozygous-KO cells, with a consistent decrease in apoB production and secretion. The APOB-p.Leu351Arg homozygous cells presented with a 40% decreased APOB expression and undetectable apoB levels in cellular extracts and supernatant. Thus, the p.Leu351Arg affected the apoB secretion, which led us to classify this new variant as likely pathogenic and to set up a hepatic follow-up in this family. Therefore, the functional assessment of APOB-missense variants, using gene-editing technologies, will lead to improvements in the molecular diagnosis of FHBL and the personalized follow-up of these patients. 相似文献
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Emmanuel Porcheron Pascal Lemaitre Amandine Nuboer Vincent Rochas Jacques Vendel 《Nuclear Engineering and Design》2007,237(15-17):1862-1871
TOSQAN is an experimental program undertaken by the Institut de Radioprotection et de Sûreté Nucléaire (IRSN) in order to perform thermal hydraulic containment studies. The TOSQAN facility is a large enclosure devoted to simulate typical accidental thermal hydraulic flow conditions in nuclear-pressurized water reactor (PWR) containment. The TOSQAN facility which is highly instrumented with non-intrusive optical diagnostics is particularly adapted to nuclear safety CFD code validation. The present work is devoted to studying the interaction of a water spray injection used as a mitigation means in order to reduce the gas pressure and temperature in the containment, to produce gases mixing and washout of fission products. In order to have a better understanding of heat and mass transfers between spray droplets and the gas mixture, and to analyze mixing effects due to spray activation, we perform detailed characterization of the two-phase flow. 相似文献
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Véronique Jollet Flora Chambon Franck Rataboul Amandine Cabiac Catherine Pinel Emmanuelle Guillon Nadine Essayem 《Topics in Catalysis》2010,53(15-18):1254-1257
The dissolution of cellulose under 5 MPa of H2 in the absence of catalyst is temperature and time dependant. The presence of Pt/γ-Al2O3 increases the initial rate of dissolution. The presence of H2/Pt is essential although its exact role has not been well elucidated. 相似文献
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Poly(butylene succinate‐co‐butylene adipate)/polyethylene oxide blends for controlled release materials: A morphological study 下载免费PDF全文
Amandine Cottaz Fadi Khalil Sophie Galland Fouzia Jbilou Isabelle Adt Pascal Degraeve Catherine Joly 《应用聚合物科学杂志》2016,133(3)
Varying the formulation and processing conditions of polymer blends allows the design of materials with a large range of morphologies. Active materials embedding active compounds in a devoted phase are promising applications of such blends, offering possible various transport properties. In this study, 13 poly(butylene succinate‐co‐butylene adipate) (PBSA)/polyethylene oxide (PEO) blends were extruded in a slit die. Their morphologies were characterized by water extraction (selective PEO dissolution), FTIR spectroscopy, and differential scanning calorimetry. Transport properties were assessed by water vapor permeation and fluorescein release as model migrant. Indeed, the desorption in water of fluorescein (previously entrapped in PEO) was monitored to preliminary investigate the release properties of these materials: two morphologies were obtained (i) pseudo multilayer films made of PEO‐rich layer/PBSA‐rich layer/PEO‐rich layer and (ii) PEO nodules dispersed in the PBSA‐rich matrix for the highest PBSA contents. The first systems were erodible ones with an uncontrolled fast delivery by PEO dissolution whereas the second ones showed a controlled release by permeation through the PBSA matrix from PEO nodules. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 42874. 相似文献
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Phenolic acids composition and antioxidant activity of canola extracts in cooked beef,chicken and pork 总被引:2,自引:0,他引:2
Crude polyphenol extracts (15 or 100 mg gallic acid equivalents (GAE)/kg meat) from canola meal reduced the formation of 2-thiobarbituric acid-reactive substances (TBARS) in pre-cooked beef (66–92%), pork (43–75%) and chicken (36–70%). The canola extract contained sinapic (99.7%), ferulic (0.28%) and p-hydroxybenzoic acids (0.07%). 相似文献
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Dounia El Hamrani Amandine Chepied William Même Marc Mesnil Norah Defamie Sandra Même 《Magma (New York, N.Y.)》2018,31(4):565-576
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Using non-invasive magnetic resonance (MR) techniques and a histological approach, we assessed the outcomes of perinatal exposure at a low dose of 3,3′-DCBPA (2-chloro-4-[1-(3-chloro-4-hydroxyphenyl)-1-methylethyl]phenol) and/or 3,5-DCBPA (2,6-dichloro-4-[1-(4-hydroxyphenyl)-1-methylethyl]phenol) on mice livers.Materials and methods
Fertilized female Swiss mice were injected intraperitoneally during gestation and lactation with either vehicle control, 20 μg/kg/day of BPA, 3,5-DCBPA, 3,3′-DCBPA or a mixture (mix-DCBPA). Complementary methods were used to evaluate, in male and female pups, (1) liver structure by texture analysis of images obtained through MR imaging (MRI) and histology, (2) hepatic lipid composition through in vivo 1H MR spectroscopy (1H MRS).Results
Principal component analysis of texture parameters showed no structural modification of the liver with BPA and DCBPA treatments. Accordingly, no hepatic microvesicular steatosis was observed through hematoxylin–eosin staining. Compared to control, MRS revealed no difference in lipid composition for BPA, 3,5-DCBPA or 3,3′-DCBPA groups. However, MRS detected a significant increase in the mix-DCBPA groups for the saturated component of fatty acids (FA), total unsaturated FA bond index and polyunsaturated FA bond index.Conclusion
Prior to any structural changes, polyunsaturated fatty acids significantly increased in young male and female mice exposed perinatally at a low dose to a mixture of dichlorinated BPA.10.
G Doronzo M Viretto I Russo L Mattiello G Anfossi M Trovati 《International journal of molecular sciences》2012,13(8):9478-9488
Type 1 diabetes is characterized by insulin deficiency, type 2 by both insulin deficiency and insulin resistance: in both conditions, hyperglycaemia is accompanied by an increased cardiovascular risk, due to increased atherosclerotic plaque formation/instabilization and impaired collateral vessel formation. An important factor in these phenomena is the Vascular Endothelial Growth Factor (VEGF), a molecule produced also by Vascular Smooth Muscle Cells (VSMC). We aimed at evaluating the role of high glucose on VEGF-A(164) synthesis and secretion in VSMC from lean insulin-sensitive and obese insulin-resistant Zucker rats (LZR and OZR). In cultured aortic VSMC from LZR and OZR incubated for 24 h with d-glucose (5.5, 15 and 25 mM) or with the osmotic controls l-glucose and mannitol, we measured VEGF-A(164) synthesis (western, blotting) and secretion (western blotting and ELISA). We observed that: (i) d-glucose dose-dependently increases VEGF-A(164) synthesis and secretion in VSMC from LZR and OZR (n = 6, ANOVA p = 0.002-0.0001); (ii) all the effects of 15 and 25 mM d-glucose are attenuated in VSMC from OZR vs. LZR (p = 0.0001); (iii) l-glucose and mannitol reproduce the VEGF-A(164) modulation induced by d-glucose in VSMC from both LZR and OZR. Thus, glucose increases via an osmotic mechanism VEGF synthesis and secretion in VSMC, an effect attenuated in the presence of insulin resistance. 相似文献