Connection‐Strength Estimation of Neuronal Networks by Fitting for Izhikevich Model

Recently, there has been abundant research using multineuron recording, but there are many problems with extracting the features from the obtained spike time series, which are huge in volume and complex. Here we introduce a new method of estimating synaptic connection strengths between neurons by fitting to the Izhikevich model by maximum likelihood estimation. We demonstrate that our method can estimate connection strengths from spike time series given by a simulated neural ensemble and can estimate nonconnectivity between two independent cultured neuronal networks. These results suggest that our method is applicable to network and plasticity analysis of neuronal networks.     

Preparation of Lanthanum Nickel Oxide-Coated Ni Sheet Anodes and Their Application to Electrolytic Production of (CF3)3N in (CH3)4NF·4.0HF Melt

A new process for electrolytic production of a perfluorinated compound, (CF₃)₃N, using lanthanum nickel oxide-coated Ni sheet anode in the (CH₃)₄NF·4.0HF melt at room temperature, was developed. Thin films of the lanthanum nickel oxides were prepared on Ni sheets by sol-gel coating method using polyvinlylpyrrolidone(PVP). The main components of the thin films were La₂O₃, LaNiO₃, and La₂NiO₄ at 500, 750 and 1000 °C, respectively. The anode performance in the (CH₃)₄NF·4.0HF melt depends greatly on the main component of the thin film, and the LaNiO₃-coated Ni sheet anode gives the best anode performance. The potential of LaNiO₃-coated Ni sheet anode remains constant at 5.9 V during electrolysis at 20 mA·cm⁻² in the (CH₃)4NF·4.0HF melt for 100 h. This is because LaNiO₃ and NiF₃, and/or Ni₂F₅, the latter of which was formed during electrolysis, in the film give a high electronic conductivity to the surface film during electrolysis. The maximum mole fraction of (CF₃)₃N (21.4%) was obtained at 20 mA·cm⁻² in (CH₃)₄NF·4.0HF melt using the LaNiO₃-coated Ni sheet.     

Hydrophilic surface modification of polypropylene films by CCl4 plasma

The CCl₄ plasma treatment of polypropylene films was investigated from the viewpoint of hydrophilic surface modification using a contact-angle meter, ATR FTIR spectroscopy, and angular XPS. Hydrophilicity and chemical composition of the CCl₄ plasma treatment was effective in hydrophilic modification. The advancing contact angle of water for polypropylene films was decreased from 99° to 81-7° by the CCl₄ plasma treatment, and the surface energy increased from 27.2 to 38.9-67.7 mJ/m² when the discharge current varied from 50 to 150 mA. The CCl₄ plasma initiated chlorination, oxidation, and aluminum sputtering reactions. The chlorination of polypropylene films was favorable in a mild CCl₄ plasma operated at discharge current of 50 mA. The oxidation and aluminum sputtering reactions were predominant over the chlorination in strong CCl₄ plasmas operated at discharge currents of more than 75 mA. The chlorination initiated by the mild CCl₄ plasma was restricted near the film surface within 36 Å deep. The regions modified with the strong CCl₄ plasma reached inner layers of 36-49 Å deep. Hydrophilicity caused by the CCl₄ plasma treatment may be due not only to chlorine functionalities but also to oxygen and aluminum functionalities. © 1993 John Wiley & Sons, Inc.     

Generalized crack initiation and crack damage stress thresholds of brittle rock masses near underground excavations

The rock mass failure process is characterized by several distinct deformation stages which include crack initiation, crack propagation and coalescence. It is important to know the stress levels associated with these deformation stages for engineering design and practice.Extensive theoretical, experimental and numerical studies on the failure process of intact rocks exist. It is generally understood that crack initiation starts at 0.3 to 0.5 times the peak uniaxial compressive stress. In confined conditions, the constant-deviatoric stress criterion was found to describe the crack initiation stress level.Here, generalized crack initiation and crack damage thresholds of rock masses are proposed. The crack initiation threshold is defined by σ₁−σ₃=A σ_cm and the crack damage threshold is defined by σ₁−σ₃=B σ_cm for jointed rock masses, where A and B are material constants and σ_cm is the uniaxial compressive strength of the rock masses. For a massive rock mass without joints, σ_cm is equal to σ_cd, the long-term uniaxial strength of intact rock. After examining data from intact rocks and jointed rock masses, it was found that for massive to moderately jointed rock masses, the material constants A and B are in the range of 0.4 to 0.5, 0.8 to 0.9, respectively, and for moderately to highly jointed rock masses, A and B are in the range of 0.5 to 0.6, 0.9 to 1.0, respectively. The generalized crack initiation and crack damage thresholds, when combined with simple linear elastic stress analysis, assist in assessing the rock mass integrity in low confinement conditions, greatly reducing the effort needed to obtain the required material constants for engineering design of underground excavations.     

Performances of metal fluoride added carbon anodes with pre-electrolysis for electrolytic synthesis of NF3

Metal fluoride added carbon anodes treated by pre-electrolysis were investigated for electrolytic production of nitrogen trifluoride (NF₃) in molten NH₄F·KF·4HF at 100 °C. The conditions for pre-electrolysis were first optimized using a graphite sheet anode as a model anode. The formation of fluorine-graphite intercalation compounds (fluorine-GICs) with semi-covalent C–F bonds, (C_xF)_n, on the MgF₂ and CaF₂ added carbon anode surface was accelerated by pre-electrolysis at potentials less than 4.0 V. Critical current densities (CCD) on the MgF₂ added carbon anodes pre-electrolyzed under various conditions were determined, and the highest CCD was 290 mA cm⁻² obtained for that pre-electrolyzed at 3.5 V for 500 C cm⁻². This anode was successfully used in the electrolysis at 100 mA cm⁻² for 290 h and the maximum NF₃ current efficiency was 55%. From these results, it was concluded that the metal fluoride added carbon anode treated by pre-electrolysis has a high potential for electrolytic production of NF₃ at higher current density.     

Structural studies of enzyme-based microfluidic biofuel cells

An enzyme-based glucose/O₂ biofuel cell was constructed within a microfluidic channel to study the influence of electrode configuration and fluidic channel height on cell performance. The cell was composed of a bilirubin oxidase (BOD)-adsorbed O₂ cathode and a glucose anode prepared by co-immobilization of glucose dehydrogenase (GDH), diaphorase (Dp) and VK₃-pendant poly-l-lysine. The consumption of O₂ at the upstream cathode protected the downstream anode from interfering O₂ molecules, and consequently improved the cell performance (maximum cell current) ca. 10% for the present cell. The cell performance was also affected by the channel height. The output current and power of a 0.1 mm-height cell was significantly less than those of a 1 mm-height cell because of the depletion of O₂, as determined by the shape of the E–I curve at the cathode. On the other hand, the volume density of current and power was several times higher for the narrower cell.     

CO gas sensor devices plasma-polymerized from tetramethyltin

Thin films plasma-polymerized from tetramethyltin was applied for CO gas sensor device. The films formed from tetramethyltin contains alkyl chains with organic tin moieties, and pyrolysis of them at 350–500°C yields carbonized films with SnO₂ moieties. The electrical resistance for the films pyrolized at 350–500°C decreases in exposing to CO gas. The sensitivity, the ratio of the electrical resistance between in air and in CO atmosphere, is enhanced by catalytic actions of palladium chloride, specially in operation at low temperatures below 50°C. The gas sensitivity between CO and other gases such as ethanol, methane, and propane gases is good. The possible determination of CO gas concentration by the sensor device is in ranges from 10 to 1000 ppm.     

Mechanism‐Based Trapping of the Quinonoid Intermediate by Using the K276R Mutant of PLP‐Dependent 3‐Aminobenzoate Synthase PctV in the Biosynthesis of Pactamycin

Mutational analysis of the pyridoxal 5′‐phosphate (PLP)‐dependent enzyme PctV was carried out to elucidate the multi‐step reaction mechanism for the formation of 3‐aminobenzoate (3‐ABA) from 3‐dehydroshikimate (3‐DSA). Introduction of mutation K276R led to the accumulation of a quinonoid intermediate with an absorption maximum at 580 nm after the reaction of pyridoxamine 5′‐phosphate (PMP) with 3‐DSA. The chemical structure of this intermediate was supported by X‐ray crystallographic analysis of the complex formed between the K276R mutant and the quinonoid intermediate. These results clearly show that a quinonoid intermediate is involved in the formation of 3‐ABA. They also indicate that Lys276 (in the active site of PctV) plays multiple roles, including acid/base catalysis during the dehydration reaction of the quinonoid intermediate.