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Wongkongkatep P Manopwisedjaroen K Tiposoth P Archakunakorn S Pongtharangkul T Suphantharika M Honda K Hamachi I Wongkongkatep J 《Langmuir : the ACS journal of surfaces and colloids》2012,28(13):5729-5736
An oil-in-water Pickering emulsion stabilized by biobased material based on a bacteria-chitosan network (BCN) was developed for the first time in this study. The formation of self-assembled BCN was possible due to the electrostatic interaction between negatively charged bacterial cells and polycationic chitosan. The BCN was proven to stabilize the tetradecane/water interface, promoting formation of highly stable oil-in-water emulsion (o/w emulsion). We characterized and visualized the BCN stabilized o/w emulsions by scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). Due to the sustainability and low environmental impact of chitosan, the BCN-based emulsions open up opportunities for the development of an environmental friendly new interface material as well as the novel type of microreactor utilizing bacterial cells network. 相似文献
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Akio Ojida Yoshifumi Miyahara Jirarut Wongkongkatep Shun‐ichi Tamaru Kazuki Sada Itaru Hamachi 《化学:亚洲杂志》2006,1(4):555-563
Nucleoside pyrophosphate (nucleoside PP) derivatives are widespread in living cells and play pivotal roles in various biological events. We report novel fluorescence chemosensors for nucleoside PPs that make use of coordination chemistry. The chemosensors, which contain two ZnII–dipicolylamine units, bind strongly to nucleoside PPs (Kapp>106 M ?1) in aqueous solution and sense them by a dual‐emission change. Detailed fluorescence and UV/Vis spectral studies revealed that the emission changes of the chemosensors upon binding to nucleoside PPs can be ascribed to the loss of coordination between ZnII and the acridine fluorophore. This is a unique sensing system based on the anion‐induced rearrangement of the coordination. Furthermore, we demonstrated the utility of these chemosensors in real‐time monitoring of two important biological processes involving nucleoside PP conversion: the apyrase‐catalyzed hydrolysis of nucleoside PPs and the glycosyl transfer catalyzed by β‐1,4‐galactosyltransferase. 相似文献
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