鸭肝癌癌基因的研究——mht(raf)及Ha-ras

本文首次报道了鸭肝癌及癌旁组织DNA对NIH 3T3及RAT-1细胞的转化活性,并获得了转化细胞株。从转化细胞株DNA分析,证明鸭肝癌和癌旁DNA的转化细胞株中均存在mht(mil或raf)和C-Ha-ras的顺序。这对进一步研究乙型肝炎病毒,化学致癌物引发鸭肝癌的分子机理提供了重要的基础。     

TRANSFORMING GENES IN DUCK HEPATIC CARCINOMA——mht(raf) AND Ha-ras

The transfection of NIH 3T3 cells was performed with DNAs from 2 duck primary hepatic carcinomas (DHC 40K, 9K) and I tumor-adjacent liver tissue (TAL, 9N). Transfectants were found from 40K, 9K and 9N DNAs. The secondary transfectants were obtained after transfection of RAT-1 cells with DNAs from primary transfectants. After hybridization with Ha-ras, Ki-ras, N-ras and mht oncogenes, it was found that duck mht (5.2 and 3.2 kb EcoRI fragments) and duck Ha-ras (3.4 kb EcoRI fragments) were present in all these transformants.This is the first report on transforming genes in duck primary hepatic cancer as well as tumoradjacent liver tissue