N-3-甲基丁基-N-1-甲基丙酮基亚硝胺体外恶性转化金仓鼠肺成纤维细胞的研究

一种存在于林县霉变玉米面中的新亚硝胺化合物——N-3-甲基丁基-N-1-甲基丙酮基亚硝胺(MAMBNA)已人工合成。利用新生金仓鼠肺成纤维细胞体外恶性转化模型,引入微粒体酶代谢激活系统检测了MAMBNA。实验结果,MAMBNA同已知的强化学致癌物N-甲基-N'-硝基-N-亚硝基胍和二乙基亚硝胺一样,能使体外培养的金仓鼠肺成纤维细胞发生恶性转化。证明MAMBNA是林县这一高癌地区的一种环境致癌物。本系统除可用于检测可疑环境致癌物外,尚可作为研究癌变机理的体外模型。     

MALIGNANT TRANSFORMATION OF BABY HAMSTER LUNG FIBROBLASTS INDUCED IN VITRO BY A NEW NITROSAMINE COMPOUND

A new nitrosamine compound, N-1-methylacetonyl-N-3-methylbutyl-nitrosamine (MAMB-NA), isolated from corn bread inoculated with moulds commonly occurring in Linxian County,a high incidence area for esophageal cancer in North China was tested for its potentialcarcinogenicity in vitro. Baby hamster lung fibroblasts (BHL) were treated with MAMBNAin tissue culture medium containing liver microsome preparation of Aroclor 1254 treatedrat. Cell transformation was assessed by morphological changes, loss of density-dependentinhibition and unlimited growth in vitro, chromosome changes, colony formation on softagar, growth at low serum concentration, Con A agglutinability and tumor formation afterheterotransplantation into immunosuppressed newborne rats. Like the known carcinogensN-methyl-N'-nitro-N-nitroso-guanidine and diethylnitrosamine, MAMBNA was able to causemalignant transformation of cells cultured in vitro.     

体外化学诱发人食管成纤维细胞恶性转化

从一例52岁食管鳞癌患者的食管组织得到的原代成纤维细胞.经用化学致癌物(N-methyl-N’-nitro-N-nitrosoguanidine,MNNG)处理后。诱发了恶性转化.细胞显示恶性细胞的生物学及形态学特性,如失去接触抑制、体外无限制的生长、非整倍体性、ConA凝集性、细胞表面微绒毛形成,在琼脂固体培养基上生长,在免疫抑制的新生小鼠上接种形成肿瘤(低分化纤维肉瘤).